The structure and function of the β2-adaptin appendage domain

EMBO Journal

Volumn 19, Issue 16, 2000, Pages 4216-4227

  Owen, D J ^a   Vallis, Y ^a   Pearse, B M F ^a   McMahon, H T ^a   Evans, P R ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

Adaptins; Clathrin; Endocytosis; Eps 15; Vesicle formation

Indexed keywords

ADAPTOR PROTEIN; CLATHRIN; PROTEIN SUBUNIT; TETRAMER;

ANIMAL CELL; ARTICLE; COATED VESICLE; CONTROLLED STUDY; ENDOCYTOSIS; HUMAN; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

ADAPTOR PROTEIN COMPLEX BETA SUBUNITS; ADAPTOR PROTEIN COMPLEX DELTA SUBUNITS; ADAPTOR PROTEINS, VESICULAR TRANSPORT; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; BRAIN CHEMISTRY; CALCIUM-BINDING PROTEINS; CARRIER PROTEINS; CIRCULAR DICHROISM; CLATHRIN; COS CELLS; CRYSTALLOGRAPHY, X-RAY; DNA, COMPLEMENTARY; ENDOCYTOSIS; GLUTATHIONE TRANSFERASE; HUMANS; IMMUNOHISTOCHEMISTRY; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MEMBRANE PROTEINS; MICROSCOPY, ELECTRON; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NEUROPEPTIDES; PHOSPHOPROTEINS; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP; SWINE; VESICULAR TRANSPORT PROTEINS;

ANIMALIA;

EID: 0034663990     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Abrahams, J.P. and Leslie, A.G.W. (1996) Methods used in the structure determination of bovine mitochondrial F1 ATPase. Acta Crystallogr. D, 52, 30-42.
2. Benmerah, A., Gagnon, J., Begue, B., Megarbane, B., Dautry-Varsat, A. and Cerf-Bensussan, N. (1995) The tyrosine kinase substrate eps15 is constitutively associated with the plasma membrane adaptor AP2. J. Cell Biol., 131, 1831-1838.
3. Chen, K. Fre, S., Slepnev, V.I., Capua, M.R., Takei, K., Butler, M.H., Di Fiore, P.P. and De Camilli, P. (1998) Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis. Nature, 394, 793-797.
4. Collaborative Computing Project No. 4 (1994) The CCP4 suite: programs for protein crystallography. Acta Crystallogr. D, 50, 760-763.
5. Cupers, P., Jadhav, A. and Kirchhausen, T. (1998) Assembly of clathrin coats disrupts the association between Eps15 and AP2 adaptors. J. Biol. Chem., 273, 1847-1850.
6. De Beer, T., Carter, R., Lobel-Rice, K.E., Sorkin, A. and Overduin, M. (1998) Structure and asn-pro-phe binding pocket of the eps15 homology domain. Science, 281, 1357-1360.
7. de la Fortelle, E. and Bricogne, G. (1997) Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods. Methods Enzymol., 276, 472-494.
8. Dell'Angelica, E., Klumperman, J., Stoorvogel, W. and Bonifacino, J. (1998) Association of the AP-3 adaptor complex with clathrin. Science, 280, 431-434.
9. Drake, M., Downs, M. and Traub, L. (2000) Epsin binds to clathrin by associating directly with the clathrin-terminal domain. J. Biol. Chem., 275, 6479-6489.
10. Gaidarov, I. and Keen, J. (1999) Phosphoinisitide-AP2 interactions required for targetting to plasma membrane clathrin-coated pits. J. Cell Biol., 146, 755-764.
11. Gaidarov, I., Santini, F., Warren, R. and Keen, J. (1999) Spatial control of coated-pit dynamics in living cells. Nature Cell Biol., 1, 1-7.
12. Goodford, P. (1996) Multivariate characterisation of molecules for QSAR. J. Chemometrics, 10, 107-117.
13. Goud, B., Huet, C. and Louvard, D. (1985) Assembled and unassembled pools of clathrin: a quantitative study using an enzyme immunoassay. J. Cell Biol., 100, 521-527.
14. Greene, B., Liu, S.-H., Wilde, A. and Brodsky, F.M. (2000) Complete reconstitution of clathrin basket formation with recombinant protein fragments: Adaptor control of clathrin self-assembly. Traffic, 1, 69-75.
15. Hao, W., Luo, Z., Zheng, L., Prasad, K. and Lafer, E.M. (1999) AP180 and AP2 interact directly in a complex that cooperatively assembles clathrin. J. Biol. Chem., 274, 22785-22794.
16. Hirst, J. and Robinson, M.S. (1998) Clathrin and adaptors. Biochim. Biophys. Acta, 1404, 173-193.
17. Iannolo, G., Salcini, A.E., Gaidarov, I., Goodman, O.B., Baulida, J. and Carpenter, G. (1997) Mapping of the molecular determinants involved in the interaction between eps15 and AP2. Cancer Res., 57, 240-245.
18. Jones, T.A., Zou, J.Y., Cowan, S.W. and Kjeldgaard, M. (1991) Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A, 47, 110-119.
19. Kirchhausen, T. (1999) Adaptors for clathrin-mediated traffic. Annu. Rev. Cell. Dev. Biol., 15, 705-732.
20. Kirchhausen, T., Nathanson, K.L., Matsui, W., Vaisberg, A., Chow, E.P., Burne, C., Keen, J.H. and Davis, A.E. (1989) Structural and functional division into two domains of the large (100-115kDa) chains of the clathrin-associated protein complex AP2. Proc. Natl Acad. Sci. USA, 86, 2612-2616.
21. Leslie, A.G. W. (1992) Recent changes to the MOSFLM package for processing film and image plate data. In, Joint CCP4 and ESF-EACMB Newsletter on Protein Crystallography No. 26. SERC, Daresbury Laboratory, Warrington, UK.
22. Mellman, I. and Warren, G. (2000) The road taken: past and future foundations of membrane traffic. Cell, 100, 99-112.
23. Morris, S., Schroder, S., Plessmann, U., Weber, K. and Ungewickell, E. (1993) Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site. EMBO J., 12, 667-675.
24. Morris, S.A., Mann, A. and Ungewickell, E. (1990) Analysis of 100-180kDa phosphoproteins in clathrin-coated vesicles from bovine brain. J. Biol. Chem., 265, 3354-3357.
25. Murphy, J. and Keen, J. (1992) recognition sites for clathrin-associated protein AP2 and protein AP3 on clathrin triskelia. J. Biol. Chem., 267, 10850-10855.
26. Murshudov, G.N., Vagin, A.A. and Dodson, E.J. (1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr. D, 53, 240-255.
27. Nicholls, A., Sharp, K.A. and Honig, B. (1991) Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins, 11, 281-296.
28. Ohno, H. et al. (1995) Interaction of tyrosine-based sorting signals with clathrin-associated proteins. Science, 269, 1872-1875.
29. Owen, D.J. and Evans, P.R. (1998) A structural explanation for the recognition of tyrosine based endocytic signals. Science, 282, 1327-1332.
30. Owen, D.J., Wigge, P., Vallis, Y., Moore, J.D.A., Evans, P.R. and McMahon, H.T. (1998) Crystal structure of the amphiphysin2 SH3 domain and its role in the prevention of dynamin ring formation. EMBO J., 17, 5273-5285.
31. Owen, D.J., Vallis, Y., Noble, M.E.M., Hunter, J.B., Dafforn, T.R., Evans, P.R. and McMahon, H.T. (1999) A structural explanation for the binding of multiple ligands by the α-adaptin appendage domain. Cell, 97, 805-815.
32. Pearse, B., Smith, C. and Owen, D. (2000) Clathrin coat construction in endocytosis. Curr. Opin. Struct. Biol., 10, 220-228.
33. Ponnambalam, S., Robinson, M., Jackson, A., Peiperl, L. and Parham, P. (1990) Conservation and diversity in families of cpated vesicle adaptins: structural analysis of β-adaptin. J. Biol. Chem., 265, 4814-4820.
34. Rapoport, I., Miyazaki, M., Boll, W., Duckworth, B., Cantley, L.C., Shoelson, S. and Kirchhausen, T. (1997) Regulatory interactions in the recognition of endocytic sorting signals by AP-2 complexes. EMBO J., 16, 2240-2250.
35. Rapoport, I., Chen, Y.,C., Cupers, P., Shoelson, S.E. and Kirchhausen, T. (1998) Dileucine-based sorting signals bind to the β chain of AP-1 at a site distinct and regulated differently form the tyrosine-based motif-binding site. EMBO J., 17, 2148-2155.
36. Roos, J. and Kelly, R. (1999) The endocytic machinery of nerve terminals surrounds sites of exocytosis. Curr. Biol., 9, 1411-1414.
37. Salcini, A., Confalonieri, S., Doria, M., Santolini, E., Tassi, E., Minenkova, O., Cesareni, G., Pelicci, P. and DiFiore, P. (1997) Binding specificty and in vivo targets of the EH domain, a novel protein-protein interaction module. Genes Dev., 11, 2239-2249.
38. Schmid, S.L. (1997) Clathrin-coated vesicle formation and protein sorting. Annu. Rev. Biochem., 66, 511-548.
39. Shih, W., Gallusser, A. and Kirchhausen, T. (1995) A clathrin-binding site in the hinge region of the β2 chain of mammalian AP2 complexes. J. Biol. Chem., 270, 31083-31090.
40. Shupliakov, O., Low, P., Grabs, D., Gad, H., Chen, H., David, C., Takei, K., De Camilli, P. and Brodin, L. (1997) Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions. Science, 276, 259-263.
41. Smith, C.J., Grigorieff, N. and Pearse, B.M.F. (1998) Clathrin coats at 21 Å resolution: a cellular assembly deigned to recycle multiple membrane receptors. EMBO J., 17, 4943-4953.
42. Ter Haar, E., Harrison, S.C. and Kirchhausen, T. (2000) Peptide-in-groove interactions link target proteins to the β-propeller of clathrin. Proc. Natl Acad. Sci. USA, 97, 1096-1100.
43. Traub, L.M. (1997) Clathrin-associated adaptor proteins putting it all together. Trends Cell Biol., 7, 43-46.
44. Traub, L.M., Downs, M.A., Westrich, J.L. and Fremont, D.H. (1999) Crystal structure of the α appendage of AP2 reveals a recruitment platform for clathrin-coat assembly. Proc. Natl Acad. Sci. USA, 96, 8907-8912.
45. Wigge, P., Vallis, Y. and McMahon, H. (1997) Inhibition of receptor-mediated endocytosis by the amphiphysin SH3 domain. Curr. Biol., 7, 554-560.
46. Wilde, A. and Brodsky, F.M. (1996) In vivo phosphorylation of adaptors regulates their interaction with clathrin. J. Cell Biol., 135, 635-645.
47. Ybe, J.A., Brodsky, F.M., Hofmann, K., Lin, K., Liu, S.-H., Chen, L., Earnest, T.N., Fletterick, R.J. and Hwang, P.K. (1999) Clathrin self-assembly is mediated by a tandemly repeated superhelix. Nature, 399, 371-375.