Assisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of spliceosomal UsnRNPs

EMBO Journal

Volumn 21, Issue 21, 2002, Pages 5853-5863

  Meister, Gunter ^a   Fischer, Utz ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

PRMT5; RNP assembly; SMN; Spliceosomes

Indexed keywords

ADENOSINE TRIPHOSPHATE; GENE PRODUCT; METHYLTRANSFERASE; PROTEIN PRMT5; SMALL NUCLEAR RIBONUCLEOPROTEIN; SURVIVAL MOTOR NEURON PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; HUMAN; HUMAN CELL; METHYLATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; SPINAL MUSCULAR ATROPHY; SPLICEOSOME;

ANIMALS; BLOTTING, WESTERN; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; HELA CELLS; HUMANS; MICROINJECTIONS; NERVE TISSUE PROTEINS; PRECIPITIN TESTS; PROTEIN BINDING; PROTEIN METHYLTRANSFERASES; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA-BINDING PROTEINS; SPLICEOSOMES; XENOPUS LAEVIS;

EID: 0036845335     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf585     Document Type: Article

References (36)

1. Brahms, H., Meheus, L., de Brabandere, V., Fischer, U. and Lührmann, R. (2001) Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B′ and the Sm-like protein LSm4 and their interaction with the SMN protein. RNA, 7, 1-12.
2. Bringmann, P. and Luhrmann, R. (1986) Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents. EMBO J., 5, 3509-3516.
3. Buhler, D., Raker, V., Luhrmann, R. and Fischer, U. (1999) Essential role for the tudor domain of SMN in spliceosomal U snRNP assembly: Implications for spinal muscular atrophy. Hum. Mol. Genet., 8, 2351-2357.
4. Campbell, L., Hunter, K.M., Mohaghegh, P., Tinsley, J.M., Brasch, M.A. and Davies, K.E. (2000) Direct interaction of Smn with dp103, a putative RNA helicase: A role for Smn in transcription regulation? Hum. Mol. Genet., 9, 1093-1100.
5. Charroux, B., Pellizzoni, L., Perkinson, R.A., Shevchenko, A., Mann, M. and Dreyfuss, G. (1999) Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. J. Cell Biol., 147, 1181-1194.
6. Charroux, B., Pellizzoni, L., Perkinson, R.A., Yong, J., Shevchenko, A., Mann, M. and Dreyfuss, G. (2000) Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli. J. Cell Biol., 148, 1177-1186.
7. Fischer, U., Liu, Q. and Dreyfuss, G. (1997) The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis. Cell, 90, 1023-1029.
8. Friesen, W.J. and Dreyfuss, G. (2000) Specific sequences of the Sm and Sm-like (Lsm) proteins mediate their interaction with the spinal muscular atrophy disease gene product (SMN). J. Biol. Chem., 275, 26370-26375.
9. Friesen, W.J., Paushkin, S., Wyce, A., Massenet, S., Pesiridis, G.S., Van Duyne, G., Rappsilber, J., Mann, M. and Dreyfuss, G. (2001b) The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins. Mol. Cell. Biol., 21, 8289-8300.
10. Grundhoff, A.T., Kremmer, E., Türeci, Ö., Glieden, A., Gindorf, C., Atz, J., Müller-Lantzsch, N., Schubach, W.H. and Grässer, F.A. (1999) Characterisation of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C. J. Biol. Chem., 274, 19136-19144.
11. Hamm, J. and Mattaj, I.W. (1989) An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis. EMBO J., 8, 4179-4187.
12. Jones, K.W., Gorzynski, K., Hales, C.M., Fischer, U., Terns, R.M. and Terns, M.P. (2001) Direct interaction of the spinal muscular atrophy disease protein SMN with the core snoRNP protein fibrillarin. J. Biol. Chem., 276, 38645-38651.
13. Lefebvre, S. et al. (1995) Identification and characterization of a spinal muscular atrophy-determining gene. Cell, 80, 155-165.
14. Lefebvre, S., Burlet, P., Liu, Q., Bertrandy, S., Clermont, O., Munnich, A., Dreyfuss, G. and Melki, J. (1997) Correlation between severity and SMN protein level in spinal muscular atrophy. Nat. Genet., 16, 265-269.
15. Liu, Q. and Dreyfuss, G. (1996) A novel nuclear structure containing the survival of motor neurons protein. EMBO J., 15, 3555-3565.
16. Liu, Q., Fischer, U., Wang, F. and Dreyfuss, G. (1997) The spinal muscular atrophy disease gene product, SMN and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell, 90, 1013-1021.
17. Meister, G., Buhler, D., Laggerbauer, B., Zobawa, M., Lottspeich, F. and Fischer, U. (2000) Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. Hum. Mol. Genet., 9, 1977-1986.
18. Meister, G., Buhler, D., Pillai, R., Lottspeich, F. and Fischer, U. (2001a) A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs. Nat. Cell Biol., 3, 945-949.
19. Meister, G., Eggert, C., Buehler, D., Brahms, H., Kambach, C. and Fischer, U. (2001b) Methylation of Sm proteins by a complex containing PRMT5 and the putative U snRNP assembly factor pICln. Curr. Biol., 11, 1990-1994.
20. Meister, G., Eggert, C. and Fischer, U. (2002) SMN-mediated assembly of spliceosomal U snRNPs: A complex story. Trends Cell Biol., 12, 472-478.
21. Monani, U.R. et al. (2000) The human centromeric survival motor neuron gene (SMN2) rescues embryonic lethality in Smn(-/-) mice and results in a mouse with spinal muscular atrophy. Hum. Mol. Genet., 9, 333-339.
22. Mourelatos, Z., Abel, L., Yong, J., Kataoka, N. and Dreyfuss, G. (2001) SMN interacts with a novel family of hnRNP and spliceosomal proteins. EMBO J., 20, 5443-5452.
23. Pellizzoni, L., Charroux, B. and Dreyfuss, G. (1999) SMN mutants of spinal muscular atrophy patients are defective in binding to snRNP proteins. Proc. Natl. Acad. Sci. USA, 96, 11167-11172.
24. Pellizzoni, L., Baccon, J., Charroux, B. and Dreyfuss, G. (2001a) The survival of motor neurons (SMN) protein interacts with the snoRNP proteins fibrillarin and GAR1. Curr. Biol., 11, 1079-1088.
25. Pellizzoni, L., Charroux, B., Rappsilber, J., Mann, M. and Dreyfuss, G. (2001b) A functional interaction between the survival motor neuron complex and RNA polymerase II. J. Cell Biol., 152, 75-86.
26. Pellizzoni, L., Baccon, J., Rappsilber, J., Mann, M. and Dreyfuss, G. (2002) Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component. J. Biol. Chem., 277, 7540-7545.
27. Pillai, R.S., Will, C.L., Luhrmann, R., Schumperli, D. and Muller, B. (2001) Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein. EMBO J., 20, 5470-5479.
28. Pu, W.T., Krapivinsky, G.B., Krapivinsky, L. and Clapham, D.E. (1999) pICln inhibits snRNP biogenesis by binding core spliceosomal proteins. Mol. Cell. Biol., 19, 4113-4120.
29. Raker, V.A., Plessel, G. and Luhrmann, R. (1996) The snRNP core assembly pathway: Identification of stable core protein heteromeric complexes and an snRNP subcore particle in vitro. EMBO J., 15, 2256-2269.
30. Raker, V.A., Hartmuth, K., Kastner, B. and Luhrmann, R. (1999) Spliceosomal U snRNP core assembly: Sm proteins assemble onto an Sm site RNA nonanucleotide in a specific and thermodynamically stable manner. Mol. Cell. Biol., 19, 6554-6565.
31. Rossoll, W., Kroning, A.K., Ohndorf, U.M., Steegborn, C., Jablonka, S. and Sendtner, M. (2002) Specific interaction of Smn, the spinal muscular atrophy determining gene product, with hnRNP-R and gry-rbp/hnRNP-Q: A role for Smn in RNA processing in motor axons? Hum. Mol. Genet., 11, 93-105.
32. Schmalbruch, H. and Haase, G. (2001) Spinal muscular atrophy: Present state. Brain Pathol., 11, 231-247.
33. Sendtner, M. (2001) Molecular mechanisms in spinal muscular atrophy: Models and perspectives. Curr. Opin. Neurol., 14, 629-634.
34. Toro, I., Thore, S., Mayer, C., Basquin, J., Seraphin, B. and Suck, D. (2001) RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex. EMBO J., 20, 2293-2303.
35. Urlaub, H., Raker, V.A., Kostka, S. and Luhrmann, R. (2001) Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure. EMBO J., 20, 187-196.
36. Will, C.L. and Luhrmann, R. (2001) Spliceosomal UsnRNP biogenesis, structure and function. Curr. Opin. Cell Biol., 13, 290-301.