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The authors report the substrate specificity of N-acetylglucosamine-6-O-sulfotransferase-1 (GlcNAc6ST-1), one of the candidate 6-sulfotransferases for L-selectin ligand synthesis. They show that transfection of the gene for the 6-sulfotransferase to cells confers significant L-selectin-mediated rolling, complementing the results of an earlier study [10] indicating that the same enzyme conferred significant L-selectin-mediated cell adhesion in nonstatic monolayer cell adhesion assays.
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Uchimura K., El-Fasakhany F.M., Hori M., Hemmerich S., Blink S.E., Kansas G.S., Kanamori A., Kumamoto K., Kannagi R., Muramatsu T. Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression. J Biol Chem. 277:2002;3979-3984. The authors report the substrate specificity of N-acetylglucosamine-6-O-sulfotransferase-1 (GlcNAc6ST-1), one of the candidate 6-sulfotransferases for L-selectin ligand synthesis. They show that transfection of the gene for the 6-sulfotransferase to cells confers significant L-selectin-mediated rolling, complementing the results of an earlier study [10] indicating that the same enzyme conferred significant L-selectin-mediated cell adhesion in nonstatic monolayer cell adhesion assays.
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This study indicates that disruption of the gene for HEC-GlcNAc6ST (high endothelial cell-specific 6-sulfotransferase, also called GlcNAc6ST-2) results in the profound disturbance of lymphocyte homing, showing the importance of the enzyme in the synthesis of L-selectin ligands. L-selectin-mediated homing decreased to about 50% of the level in wild-type mice.
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An excellent review covering the most recent progress in the study of the roles played by cell adhesion molecules and chemokines in various aspects of lymphocyte homing. The contribution of carbohydrate determinants is referred to using the term CLA (cutaneous lymphocyte antigen), defined by a monoclonal antibody, HECA-452, which is reactive to both 6-sulfated and nonsulfated sialyl Lewis x.
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Kunkel E.J., Butcher E.C. Chemokines and the tissue-specific migration of lymphocytes. Immunity. 16:2002;1-4. An excellent review covering the most recent progress in the study of the roles played by cell adhesion molecules and chemokines in various aspects of lymphocyte homing. The contribution of carbohydrate determinants is referred to using the term CLA (cutaneous lymphocyte antigen), defined by a monoclonal antibody, HECA-452, which is reactive to both 6-sulfated and nonsulfated sialyl Lewis x.
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A comprehensive review of over 40 chemokines, covering in detail the recently described, so-called 'immune system chemokines' or 'homeostatic chemokines' that govern routine homing of lymphocytes.
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Yoshie O., Imai T., Nomiyama H. Chemokines in immunity. Adv Immunol. 78:2001;57-110. A comprehensive review of over 40 chemokines, covering in detail the recently described, so-called 'immune system chemokines' or 'homeostatic chemokines' that govern routine homing of lymphocytes.
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The authors found that sulfated carbohydrate determinants defined by the antibody PEN5 are expressed on NK cells and serve as ligands for L-selectin.
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Andre P., Spertini O., Guia S., Rihet P., Dignat-George F., Brailly H., Sampol J., Anderson P.J., Vivier E. Modification of P-selectin glycoprotein ligand-1 with a natural killer cell-restricted sulfated lactosamine creates an alternate ligand for L-selectin. Proc Natl Acad Sci USA. 97:2000;3400-3405. The authors found that sulfated carbohydrate determinants defined by the antibody PEN5 are expressed on NK cells and serve as ligands for L-selectin.
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