How to make tetracycline-regulated transgene expression go on and off

Analytical Biochemistry

Volumn 309, Issue 1, 2002, Pages 79-84

  Rennel, Emma ^a   Gerwins, Pär ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

Doxycycline; Gene therapy; Regulatable; Transgene; TTA responsive promoter

Indexed keywords

ANIMALS; GENE EXPRESSION; TISSUE CULTURE;

DOXYCYCLINE; EXPRESSION SYSTEM; EXTRACELLULAR MATRICES; REGULATABLE; TRANSGENE; TRANSGENE EXPRESSION; TRANSGENIC ANIMALS; TTA-RESPONSIVE PROMOTER;

GENE THERAPY;

DOXYCYCLINE; TETRACYCLINE;

ARTICLE; CELL LINE; CLONAL VARIATION; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RELIABILITY; TRANSGENE; TRANSGENIC ANIMAL; ADENOVIRUS; ANIMAL; BRAIN; CELL CULTURE; CYTOLOGY; DRUG EFFECT; GENE VECTOR; GENETIC TRANSCRIPTION; GENETICS; GLIOMA; KINETICS; METABOLISM; REPRODUCIBILITY; WESTERN BLOTTING;

ANIMALIA;

ADENOVIRIDAE; ANIMALS; BLOTTING, WESTERN; BRAIN; CELL LINE; DOXYCYCLINE; GENE EXPRESSION REGULATION; GENETIC VECTORS; GLIOMA; KINETICS; REPRODUCIBILITY OF RESULTS; TETRACYCLINE; TRANSCRIPTION, GENETIC; TRANSGENES; TUMOR CELLS, CULTURED;

EID: 0036807785     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00250-6     Document Type: Article

References (15)

1. E. Saez, D. No, A. West, R.M. Evans, Inducible gene expression in mammalian cells and transgenic mice, Curr. Opin. Biotechnol. 8 (1997) 608-616.
2. A. Kistner, M. Gossen, F. Zimmermann, J. Jerecic, C. Ullmer, H. Lubbert, H. Bujard, Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice, Proc. Natl. Acad. Sci. USA 93 (1996) 10933-10938.
3. S. Yla-Herttuala, J.F. Martin, Cardiovascular gene therapy, Lancet. 355 (2000) 213-222.
4. R.J. Lee, M.L. Springer, W.E. Blanco-Bose, R. Shaw, P.C. Ursell, H.M. Blau, VEGF gene delivery to myocardium: Deleterious effects of unregulated expression, Circulation 102 (2000) 898-901.
5. M.L. Springer, A.S. Chen, P.E. Kraft, M. Bednarski, H.M. Blau, VEGF gene delivery to muscle: Potential role for vasculogenesis in adults, Mol. Cell 2 (1998) 549-558.
6. H.M. Blau, A. Banfi, The well-tempered vessel, Nature Med. 7 (2001) 532-534.
7. M. Gossen, H. Bujard, Tight control of gene expression in mammalian cells by tetracycline-responsive promoters, Proc. Natl. Acad. Sci. USA 89 (1992) 5547-5551.
8. M. Gossen, S. Freundlieb, G. Bender, G. Muller, W. Hillen, H. Bujard, Transcriptional activation by tetracyclines in mammalian cells, Science 268 (1995) 1766-1769.
9. A. Hofmann, G.P. Nolan, H.M. Blau, Rapid retroviral delivery of tetracycline-inducible genes in a single autoregulatory cassette, Proc. Natl. Acad. Sci. USA 93 (1996) 5185-5190.
10. S.L. Campbell, R. Khosravi-Far, K.L. Rossman, G.J. Clark, C.J. Der, Increasing complexity of Ras signaling, Oncogene 17 (1998) 1395-1413.
11. S. Kanda, E. Landgren, M. Ljungstrom, L. Claesson-Welsh, Fibroblast growth factor receptor 1-induced differentiation of endothelial cell line established from tsA58 large T transgenic mice, Cell Growth Differ. 7 (1996) 383-395.
12. S. Mohammadi, L. Alvarez-Vallina, L.J. Ashworth, R.E. Hawkins, Delay in resumption of the activity of tetracycline-regulatable promoter following removal of tetracycline analogues, Gene. Ther. 4 (1997) 993-997.
13. L. Uhrbom, M. Nister, B. Westermark, Induction of senescence in human malignant glioma cells by p16INK4A, Oncogene 15 (1997) 505-514.
14. P. Honkakoski, I. Jaaskelainen, M. Kortelahti, A. Urtti, A novel drug-regulated gene expression system based on the nuclear receptor constitutive androstane receptor (CAR), Pharm. Res. 18 (2001) 146-150.
15. K. Iwaki, N. Okumura, M. Yamazaki, Rapid determination of tetracycline antibiotics in serum by reversed-phase high-performance liquid chromatography with fluorescence detection, J. Chromatogr. 619 (1993) 319-323.