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Volumn 287, Issue 5456, 2000, Pages 1276-1279

Mitochondrial FtsZ in a chromophyte alga

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN;

EID: 0034681472     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.287.5456.1276     Document Type: Article
Times cited : (145)

References (33)
  • 2
    • 0028047262 scopus 로고
    • J. Bereiter-Hahn and M. Vöth, Microsc. Res. Tech. 27, 198 (1994); K. P. Gaffal, Endocyt. Cell Res. 4, 41 (1987).
    • (1987) Endocyt. Cell Res. , vol.4 , pp. 41
    • Gaffal, K.P.1
  • 3
    • 0029149042 scopus 로고
    • K. W. Osteryoung and E. Vierling, Nature 376, 473 (1995); K. W. Osteryoung, K. D. Stokes, S. M. Rutherford, A. L. Percival, W. Y. Lee, Plant Cell 10, 1991 (1998); R. Strepp, S. Scholz, S. Kruse, V. Speth, R. Reski, Proc. Natl. Acad. Sci. U.S.A. 95, 4368 (1998).
    • (1995) Nature , vol.376 , pp. 473
    • Osteryoung, K.W.1    Vierling, E.2
  • 5
  • 6
    • 0026059127 scopus 로고
    • E. Bi and J. Lutkenhaus, Nature 354, 161 (1991); D. Bramhill, Annu. Rev. Cell Dev. Biol. 13, 395 (1997); H. P. Erickson, Trends Cell Biol. 7, 362 (1997); J. Lutkenhaus and S. G. Addinall, Annu. Rev. Biochem. 66, 93 (1997); J. Löwe and L. A. Amos, Nature 391, 203 (1998); W. Margolin, Trends Microbiol. 6, 233 (1998).
    • (1991) Nature , vol.354 , pp. 161
    • Bi, E.1    Lutkenhaus, J.2
  • 7
    • 0031466635 scopus 로고    scopus 로고
    • E. Bi and J. Lutkenhaus, Nature 354, 161 (1991); D. Bramhill, Annu. Rev. Cell Dev. Biol. 13, 395 (1997); H. P. Erickson, Trends Cell Biol. 7, 362 (1997); J. Lutkenhaus and S. G. Addinall, Annu. Rev. Biochem. 66, 93 (1997); J. Löwe and L. A. Amos, Nature 391, 203 (1998); W. Margolin, Trends Microbiol. 6, 233 (1998).
    • (1997) Annu. Rev. Cell Dev. Biol. , vol.13 , pp. 395
    • Bramhill, D.1
  • 8
    • 0030829601 scopus 로고    scopus 로고
    • E. Bi and J. Lutkenhaus, Nature 354, 161 (1991); D. Bramhill, Annu. Rev. Cell Dev. Biol. 13, 395 (1997); H. P. Erickson, Trends Cell Biol. 7, 362 (1997); J. Lutkenhaus and S. G. Addinall, Annu. Rev. Biochem. 66, 93 (1997); J. Löwe and L. A. Amos, Nature 391, 203 (1998); W. Margolin, Trends Microbiol. 6, 233 (1998).
    • (1997) Trends Cell Biol. , vol.7 , pp. 362
    • Erickson, H.P.1
  • 9
    • 0030956145 scopus 로고    scopus 로고
    • E. Bi and J. Lutkenhaus, Nature 354, 161 (1991); D. Bramhill, Annu. Rev. Cell Dev. Biol. 13, 395 (1997); H. P. Erickson, Trends Cell Biol. 7, 362 (1997); J. Lutkenhaus and S. G. Addinall, Annu. Rev. Biochem. 66, 93 (1997); J. Löwe and L. A. Amos, Nature 391, 203 (1998); W. Margolin, Trends Microbiol. 6, 233 (1998).
    • (1997) Annu. Rev. Biochem. , vol.66 , pp. 93
    • Lutkenhaus, J.1    Addinall, S.G.2
  • 10
    • 2642593025 scopus 로고    scopus 로고
    • E. Bi and J. Lutkenhaus, Nature 354, 161 (1991); D. Bramhill, Annu. Rev. Cell Dev. Biol. 13, 395 (1997); H. P. Erickson, Trends Cell Biol. 7, 362 (1997); J. Lutkenhaus and S. G. Addinall, Annu. Rev. Biochem. 66, 93 (1997); J. Löwe and L. A. Amos, Nature 391, 203 (1998); W. Margolin, Trends Microbiol. 6, 233 (1998).
    • (1998) Nature , vol.391 , pp. 203
    • Löwe, J.1    Amos, L.A.2
  • 11
    • 0032103029 scopus 로고    scopus 로고
    • E. Bi and J. Lutkenhaus, Nature 354, 161 (1991); D. Bramhill, Annu. Rev. Cell Dev. Biol. 13, 395 (1997); H. P. Erickson, Trends Cell Biol. 7, 362 (1997); J. Lutkenhaus and S. G. Addinall, Annu. Rev. Biochem. 66, 93 (1997); J. Löwe and L. A. Amos, Nature 391, 203 (1998); W. Margolin, Trends Microbiol. 6, 233 (1998).
    • (1998) Trends Microbiol. , vol.6 , pp. 233
    • Margolin, W.1
  • 12
    • 0000590950 scopus 로고
    • Polyadenylated RNA was extracted with phenol and chloroform from an asynchronously dividing culture of M. splendens [P. L. Beech and R. Wetherbee, J. Phycol. 26, 90 (1990)] and used to construct a cDNA library with the help of a ZAP-cDNA Gigapack III Gold doning kit (Stratagene).
    • (1990) J. Phycol. , vol.26 , pp. 90
    • Beech, P.L.1    Wetherbee, R.2
  • 13
    • 0343666237 scopus 로고    scopus 로고
    • Nucleotides 60 through 1265 of AtFtsZ1-1 from A. thaliana (GenBank accession number U39877) were amplified by reverse transcription polymerase chain reaction from RNA isolated from leaves of A. thaliana (Columbia)
    • Nucleotides 60 through 1265 of AtFtsZ1-1 from A. thaliana (GenBank accession number U39877) were amplified by reverse transcription polymerase chain reaction from RNA isolated from leaves of A. thaliana (Columbia).
  • 14
    • 0025950259 scopus 로고
    • This second probe was a 535-base pair (bp) Nsi I-Mlu I fragment of the ftsZ1 gene from S. meliloti (Genbank accession number M94386) [W. Margolin, J. Corbo, S. R. Long, J. Bacteriol. 173, 5822 (1991)].
    • (1991) J. Bacteriol. , vol.173 , pp. 5822
    • Margolin, W.1    Corbo, J.2    Long, S.R.3
  • 15
    • 0032512051 scopus 로고    scopus 로고
    • S. G. E. Andersson et al., Nature 396, 133 (1998); M. W. Gray, G. Burger, B. F. Lang, Science 283, 1476 (1999).
    • (1998) Nature , vol.396 , pp. 133
    • Andersson, S.G.E.1
  • 16
    • 0033525788 scopus 로고    scopus 로고
    • S. G. E. Andersson et al., Nature 396, 133 (1998); M. W. Gray, G. Burger, B. F. Lang, Science 283, 1476 (1999).
    • (1999) Science , vol.283 , pp. 1476
    • Gray, M.W.1    Burger, G.2    Lang, B.F.3
  • 17
    • 0342796005 scopus 로고    scopus 로고
    • The sequence of MsFtsZ-mt was e-mailed for analysis with the PSORT algorithm
    • The sequence of MsFtsZ-mt was e-mailed for analysis with the PSORT algorithm (http://psort.ims.u-tokyo. ac.jp). A mitochondrial targeting probability of 0.744 was predicted.
  • 19
    • 0343666239 scopus 로고    scopus 로고
    • note
    • This behavior was seen in unpublished observations by P. L. Beech on live cells of M. splendens labeled with Mitotrackers Green FM or CMXRos (Molecular Probes) according to the manufacturer's instructions.
  • 20
    • 0342796004 scopus 로고    scopus 로고
    • note
    • An Eco RI-Spe I fragment (representing amino acids 8 through 342) of the MsFtsZ-mt cDNA was cloned into the ProEx HTb expression vector (Gibco). Six histidine fusion proteins were expressed in Escherichia coli, purified against Talon resin (Clontech), and electrophoretically separated before injection of gel slices into rabbits for antibody production. Antibodies likely to be specific to MsFtsZ-mt (anti-MsFtsZ-mt) were selected by affinity-purifying rabbit sera against the first 76 amino acids of MsFtsZ-mt [the corresponding cDNA fragment from MsFtsZ-mt was cloned into pGEX-4T-1 (Pharmacia) and expressed in E. coli as a glutathione S-transferase fusion protein] immobilized on nitrocellulose.
  • 22
    • 0033194038 scopus 로고    scopus 로고
    • W. Bleazard et al., Nature Cell Biol. 1, 298 (1999); H. Sesaki and R. E. Jensen, J. Cell Biol. 147, 699 (1999); A. M. Labrousse, M. D. Zappaterra, D. A. Rube, A. M. van der Bliek, Mol. Cell 4, 815 (1999).
    • (1999) Nature Cell Biol. , vol.1 , pp. 298
    • Bleazard, W.1
  • 23
    • 0033571410 scopus 로고    scopus 로고
    • W. Bleazard et al., Nature Cell Biol. 1, 298 (1999); H. Sesaki and R. E. Jensen, J. Cell Biol. 147, 699 (1999); A. M. Labrousse, M. D. Zappaterra, D. A. Rube, A. M. van der Bliek, Mol. Cell 4, 815 (1999).
    • (1999) J. Cell Biol. , vol.147 , pp. 699
    • Sesaki, H.1    Jensen, R.E.2
  • 26
    • 0033593816 scopus 로고    scopus 로고
    • E. Smirnova, D.-L. Shurland, S. N. Ryazantsev, A. M. van der Bliek, J. Cell Biol. 143, 351 (1998); K. A. Shepard and M. P. Yaffe, J. Cell Biol. 144, 711 (1999); M. P. Yaffe, Science 283, 1493 (1999).
    • (1999) J. Cell Biol. , vol.144 , pp. 711
    • Shepard, K.A.1    Yaffe, M.P.2
  • 27
    • 0033525615 scopus 로고    scopus 로고
    • E. Smirnova, D.-L. Shurland, S. N. Ryazantsev, A. M. van der Bliek, J. Cell Biol. 143, 351 (1998); K. A. Shepard and M. P. Yaffe, J. Cell Biol. 144, 711 (1999); M. P. Yaffe, Science 283, 1493 (1999).
    • (1999) Science , vol.283 , pp. 1493
    • Yaffe, M.P.1
  • 28
    • 0032478067 scopus 로고    scopus 로고
    • 65) isoform. S. cerevisiae cells were transformed with the MsFtsZ-mt/GFP fusion as described [R. George et al., Proc. Natl. Acad. Sci. U.S.A. 95, 2296 (1998)]. Mitochondria were isolated and subfractionated as previously described [B. S. Glick et al., Cell 69, 809 (1992)].
    • (1998) Proc. Natl. Acad. Sci. U.S.A. , vol.95 , pp. 2296
    • George, R.1
  • 29
    • 0026611680 scopus 로고
    • 65) isoform. S. cerevisiae cells were transformed with the MsFtsZ-mt/GFP fusion as described [R. George et al., Proc. Natl. Acad. Sci. U.S.A. 95, 2296 (1998)]. Mitochondria were isolated and subfractionated as previously described [B. S. Glick et al., Cell 69, 809 (1992)].
    • (1992) Cell , vol.69 , pp. 809
    • Glick, B.S.1
  • 31
    • 0342361095 scopus 로고    scopus 로고
    • note
    • Cells were examined on a Leica TCS 4D laser scanning confocal microscope under a ×100 1.3 numerical aperture objective. Signals from green (GFP or fluorescein isothiocyanate) and red (Mitotracker) channels were collected simultaneously and merged with Adobe Photoshop.
  • 32
    • 0343230599 scopus 로고    scopus 로고
    • note
    • For immunofluorescence, a nonsynchronously dividing culture of M. splendens was labeled with 100 nM MitoTracker CMX-Ros (Molecular Probes) for 2 hours; cells were washed in growth medium, then fixed in 2% paraformaldehyde in methanol at -20°C for 10 min, washed in phosphate-buffered saline (PBS), and labeled with primary antibody diluted in blocking buffer (PBS, 0.05% Tween-20, and 1% bovine serum albumin) overnight at 4°C. Primary antibodies were detected with FITC-conjugated goat anti-rabbit IgG (Selenius, Hawthorn, Victoria, Australia), and cells were observed as described (17). Seemingly identical labeling with anti-MsFtsZ-mt was achieved both with and without MitoTracker labeling. Controls for antibody labeling were performed with preimmune sera.
  • 33
    • 0343666234 scopus 로고    scopus 로고
    • note
    • We thank W. Margolin for the S. meliloti FtsZ probe, C. Cobbett for Arabidopsis RNA, J. Pickett-Heaps and R. Wetherbee for materials and encouragement, and T. Spurck for help with confocal microscopy. P.L.B. is funded by the Australian Research Council and is a Queen Elizabeth II fellow.


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