Two-hybrid system for characterization of protein-protein interactions in E. coli

BioTechniques

Volumn 29, Issue 2, 2000, Pages 288-296

  Hays, L B ^a   Chen, Y S A ^a   Hu, J C ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC PROTEIN; DNA BINDING PROTEIN; HYBRID PROTEIN; LEUCINE ZIPPER PROTEIN; PROTEIN C FOS; PROTEIN C JUN;

ARTICLE; ESCHERICHIA COLI; HYBRID; NONHUMAN; ONCOGENE C FOS; ONCOGENE C JUN; OPERATOR GENE; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; REPORTER GENE; REPRESSOR GENE;

ESCHERICHIA COLI; FELIS CATUS;

EID: 0033847495     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00292st04     Document Type: Article

References (33)

1. Finding prospective partners in the library: The two-hybrid system and phage display find a match
2. Gene identification using the yeast two-hybrid system
3. Gene identification using the yeast two-hybrid system
4. Isolation of lambda repressor mutants with defects in cooperative operator binding
5. Understanding gene and allele function with two-hybrid methods
6. Identification of a cDNA for SSRP1, an HMG-box protein, by interaction with the c-Myc oncoprotein in a novel bacterial expression screen
7. Functional domains of the AraC protein
8. Linking an easily detectable phenotype to the folding of a common structural motif. Selection of rare turn mutations that prevent the folding of Rop
9. A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli
10. Activation of prokaryotic transcription through arbitrary protein-protein contacts
11. Conversion of the ω subunit of Escherichia coli RNA polymerase into a transcriptional activator or an activation target
12. Localization of protein-protein interactions between subunits of phytochrome
13. E. coli one- and two-hybrid systems for the analysis and identification of protein-protein interactions
14. Probing the roles of residues at the e and g positions of the GCN4 leucine zipper by combinatorial mutagenesis
15. Repressor fusions as a tool to study protein-protein interactions
16. Sequence requirements for coiled-coils: Analysis with lambda repressor-GCN4 leucine zipper fusions
17. Interaction between cAMP-dependent protein kinase catalytic subunit and peptide inhibitors analyzed with λ repressor fusions
18. Genetic strategy for analyzing specificity of dimer formation: Escherichia coli cyclic AMP receptor protein mutant altered in its dimerization specificity
19. A bacterial two-hybrid system based on a reconstituted signal transduction pathway
20. Operator binding by λ repressor heterodimers with one or two N-terminal arms
21. Gene activation by the AraC protein can be inhibited by DNA looping betwen AraC and a LexA repressor that interacts with AraC: Possible applications as a two-hybrid system
22. Analysis of the Myc and Max interaction specificity with λ repressor-HLH domain fusions
23. Phage display: Protein engineering by directed evolution
24. Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments
25. Rapid confirmation of single copy lambda prophage integration by PCR
26. Construction, purification, and characterization of a hybrid protein comprising the DNA binding domain of the LexA repressor and the Jun leucine zipper: A circular dichroism and mutagenesis study
27. The leucine repeat motif in Fos protein mediates complex formation with Jun/AP-1 and is required for transformation
28. Isolation and analysis of Arc repressor mutants: Evidence for an unusual mechanism of DNA binding
29. Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing
30. Buried asparagines determine the dimerization specificities of leucine zipper mutants
31. Detection of tetramerization domains in vivo by cooperative DNA binding to tandem lambda operator sites