Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB8 is essential for the assembly of the transport pore

Molecular Microbiology

Volumn 36, Issue 3, 2000, Pages 608-617

  Kumar, Renu B ^a   Xie, Yong Hong ^a,b   Das, Anath ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b MINNEAPOLIS MEDICAL RESEARCH FOUNDATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; DNA; MEMBRANE PROTEIN;

ARTICLE; BACTERIAL MEMBRANE; BACTERIAL OUTER MEMBRANE; CELLULAR DISTRIBUTION; COMPLEX FORMATION; CONTROLLED STUDY; CYTOPLASM; DELETION MUTANT; DNA TRANSFER; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE MICROSCOPY; MEMBRANE CHANNEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RHIZOBIUM RADIOBACTER;

BACTERIAL PROTEINS; DNA, BACTERIAL; DNA, SINGLE-STRANDED; MEMBRANE PROTEINS; RHIZOBIUM RADIOBACTER; SUBCELLULAR FRACTIONS; VIRULENCE FACTORS;

AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0034059337     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.01876.x     Document Type: Article

References (42)

1. Addinall, S., Bi, E., and Lutkenhaus, J. (1996) FtsZ ring formation in fts mutants. J Bacteriol 178: 3877-3884.
2. Anderson, L.B., Hertzel, A.V., and Das, A. (1996) Agrobacterium tumefaciens VirB7 and VirB9 form a disulfide-linked protein complex. Proc Natl Acad Sci USA 93: 8889-8894.
3. Baron, C., Thorstenson, Y.R., and Zambryski, P.C. (1997) The lipoprotein VirB7 interacts with VirB9 in the membranes of Agrobacterium tumefaciens. J Bacteriol 179: 1211-1218.
4. Beaupre, C.E., Bohne, J., Dale, E.M., and Binns, A.N. (1997) Interactions between VirB9 and VirB10 membrane proteins involved in movement of DNA from Agrobacterium tumefaciens into plant cells. J Bacteriol 179: 78-89.
5. Beijersbergen, A., Dulk-Ras, A.D., Schilperoort, R.A., and Hooykaas, P.J. (1992) Conjugative transfer by the virulence system of Agrobacterium tumefaciens. Science 256: 1324-1327.
6. Beijersbergen, A., Smith, S., and Hooykaas, P.J. (1994) Localization and topology of VirB proteins of Agrobacterium tumefaciens. Plasmid 32: 212-218.
7. Berger, B., and Christie, P. (1994) Genetic complementation analysis of the Agrobacterium tumefaciens virB operon: virB2 through virB11 are essential virulence genes. J Bacteriol 176: 3646-3660.
8. Binns, A., Beaupre, C., and Dale, E. (1995) Inhibition of VirB-mediated transfer of diverse substrate from Agrobacterium tumefaciens by the IncQ plasmid RSF1010. J Bacteriol 177: 4890-4899.
9. Christie, P. (1997) Agrobacterium tumefaciens T-complex transport apparatus: a paradigm for a new family of multifunctional transporters in eubacteria. J Bacteriol 179: 3085-3094.
10. Christie, P.J., Ward, J.E.J., Gordon, M.P., and Nester, E.W. (1989) A gene required for transfer of T-DNA to plants encodes an ATPase with autophosphorylating activity. Proc Natl Acad Sci USA 86: 9677-9681.
11. Covacci, A., Telford, J.L., Del Giudice, G., Parsonnet, J., and Rappuoli, R. (1999) Helicobacter pylori virulence and genetic geography. Science 284: 1328-1333.
12. Das, A. (1998) DNA transfer from Agrobacterium to plant cells in crown gall tumor disease. In Subcellular Biochemistry: Plant Microbe Interactions. Biswas, B.B., and Das, H.K. (eds). London: Plenum Publishing Co., pp. 343-363.
13. Das, A., and Xie, Y.-H. (1998) Construction of transposon Tn3phoA: Its application in defining the membrane topology of the Agrobacterium tumefaciens DNA transfer proteins. Mol Microbiol 27: 405-414.
14. Das, A., and Xie, Y.-H. (2000) Agrobacterium tumefaciens T-DNA transport pore proteins VirB8, VirB9 and VirB10 interact with one another. J Bacteriol 182: 758-763.
15. Das, A., Anderson, L., and Xie, Y.-H. (1997) Delineation of the interaction domains of Agrobacterium tumefaciens VirB7 and VirB9 by use of the yeast two-hybrid assay. J Bacteriol 179: 3404-3409.
16. Fernandez, D., Spudich, G., Zhou, X.-R., and Christie, P. (1996) The Agrobacterium tumefaciens VirB7 lipoprotein is required for stabilization of VirB proteins during assembly of the T-complex transport apparatus. J Bacteriol 178: 3168-3176.
17. Fullner, K., Lara, J.C., and Nester, E. (1996) Pilus assembly by Agrobacterium T-DNA transfer genes. Science 273: 1107-1109.
18. Gubba, S., Xie, Y.-H., and Das, A. (1995) Regulation of Agrobacterium tumefaciens virulence gene expression: Isolation of a mutation that restores virGD52E function. Mol Plant-Micro Inter 8: 788-791.
19. Hansen, G., Das, A., and Chilton, M.-D. (1994) Constitutive expression of the virulence genes improves the efficiency of plant transformation by Agrobacterium. Proc Natl Acad Sci USA 91: 7603-7607.
20. Harlaw, E., and Lane, D. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
21. Harry, E., Pogliano, K., and Losick, R. (1995) Use of immuno-fluorescence to visualize cell-specific gene expression during sporulation in Bacillus subtilis. J Bacteriol 177: 3386-3393.
22. Ishida, Y., Saito, H., Ohta, S., Hiei, Y., Komari, T., and Kumashiro, T. (1996) High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nature Biotechnol 14: 745-750.
23. Kado, C.I. (1998) Agrobacterium-mediated horizontal gene transfer. Genetic Eng 20: 1-24.
24. Koerner, T.J., Hill, J.E., Myers, A.M., and Tzagoloff, A. (1991) High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors. Methods Enzymol 194: 477-490.
25. Kuldau, G.A., De Vos, G., Owen, J., McCaffrey, G., and Zambryski, P. (1990) The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open reading frames. Mol Gen Genet 221: 256-266.
26. Lai, E.M., and Kado, C.I. (1998) Processed VirB2 is the major subunit of the promiscuous pilus of Agrobacterium tumefaciens. J Bacteriol 180: 2711-2717.
27. Landschulz, W., Johnson, P., and McKnight, S. (1988) The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science 240: 1759-1764.
28. Leong, A.S., and Sormunen, R.T. (1998) Microwave procedures for electron microscopy and resin-embedded sections. Micron 29: 397-409.
29. Maddock, J.R., and Shapiro, L. (1993) Polar location of the chemoreceptor complex in the Escherichia coli cell. Science 259: 1717-1723.
30. Mersereau, M., Pazour, G., and Das, A. (1990) Efficient transformation of Agrobacterium tumefaciens by electroporation. Gene 90: 149-151.
31. Mohl, D.A., and Gober, J.W. (1997) Cell cycle-dependent polar localization of chromosome partitioning proteins in Caulobacter crescentus. Cell 88: 675-684.
32. Pazour, G.J., Ta, C.N., and Das, A. (1992) Constitutive mutations of Agrobacterium tumefaciens transcriptional activator virG. J Bacteriol 174: 4169-4174.
33. Schmidt-Eisenlohr, H., Domke, N., Angerer, C., Wanner, G., Zambryski, P., and Baron, C. (1999) Vir proteins stabilize VirB5 and mediate its association with the T pilus of Agrobacterium tumefaciens. J Bacteriol 181: 7485-7492.
34. Shirasu, K., Koukolikova-Nicola, Z., Hohn, B., and Kado, C. (1994) An inner-membrane associated virulence protein essential for T-DNA transfer from Agrobacterium tumefaciens to plants exhibits ATPase activity and similarity to conjugative transfer genes. Mol Microbiol 11: 581-588.
35. Spudich, G., Fernandez, D., Zhou, X.-R., and Christie, P. (1996) Intermolecular disulfide bonds stabilize VirB7 homodimers and VirB7/VirB9 heterodimers during biogenesis of the Agrobacterium tumefaciens T-complex transport pore. Proc Natl Acad Sci USA 93: 7512-7517.
36. Sundberg, C., Meek, L., Carroll, K., Das, A., and Ream, W. (1996) VirE1 protein mediates export of VirE2 single-stranded DNA binding protein from Agrobacterium tumefaciens to plant cells. J Bacteriol 178: 1207-11212.
37. Thorstenson, Y.R., and Zambryski, P.C. (1994) The essential virulence protein VirB8 localizes to the inner membrane of Agrobacterium tumefaciens. J Bacteriol 176: 1711-1717.
38. Thorstenson, Y.R., Kuldau, G.A., and Zambryski, P.C. (1993) Subcellular localization of seven VirB proteins of Agrobacterium tumefaciens - implications for the formation of a T-DNA transport structure. J Bacteriol 175: 5233-5241.
39. Vieira, J., and Messing, J. (1987) Production of single-stranded plasmid DNA. Methods Enzymol 153: 3-11.
40. Ward, J.E., Akiyoshi, D.E., Regier, D., Datta, A., Gordon, M.P., and Nester, E. (1988) Characterization of the virB operon from Agrobacterium tumefaciens Ti plasmid. [Erratum (1990) 265: 4768]. J Biol Chem 263: 5804-5814.
41. Ward, J.E., Dale, E.M., Nester, E.W., and Binns, A.N. (1990) Identification of a VirB10 protein aggregate in the inner membrane of Agrobacterium tumefaciens. J Bacteriol 172: 5200-5210.
42. Webb, C.D., Teleman, A., Gordon, S., Straight, A., Belmont, A., Lin, D., et al. (1997) Bipolar localization of the replication origin regions of chromosomes in vegetative and sporulating cells of B. subtilis. Cell 88: 667-674.