In vitro model for the study of necrosis and apoptosis in native cartilage

Journal of Pathology

Volumn 198, Issue 1, 2002, Pages 5-13

  Grogan, Shawn Patrick ^a   Aklin, Balz ^a   Frenz, Martin ^a   Brunner, Thomas ^a   Schaffner, Thomas ^a   Mainil Varlet, Pierre ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

Apoptosis; Cartilage; Caspase 3; Confocal microscopy; Ethidium homodimer; TUNEL

Indexed keywords

BIOLOGICAL MARKER; CALCEIN; CASPASE 3; DNA FRAGMENT;

AGING; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CARTILAGE; CELL DAMAGE; CELL VIABILITY; CHONDROPATHY; CONTROLLED STUDY; COW; DEVELOPMENTAL STAGE; DRUG EFFECT; ENZYME ACTIVITY; EXPERIMENTAL MODEL; HISTOLOGY; HOLMIUM LASER; IN VITRO STUDY; IN VIVO STUDY; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; NECROSIS; NICK END LABELING; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; SURGERY; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALS; APOPTOSIS; CARTILAGE, ARTICULAR; CASPASE 3; CASPASES; CATTLE; CHONDROCYTES; DNA FRAGMENTATION; IN SITU NICK-END LABELING; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; MODELS, BIOLOGICAL; NECROSIS;

EID: 0036724238     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/path.1169     Document Type: Article

References (38)

1. Apoptosis in the pathogenesis and treatment of disease
2. Apoptosis by death factor
3. Apoptotic cell death: Its implications for imaging in the next millennium
4. Fourdimensional imaging of living chondrocytes in cartilage using confocal microscopy: A pragmatic approach
5. Effect of bipolar radiofrequency energy on human articular cartilage: Comparison of confocal laser microscopy and light microscopy
6. Quantification of laser-induced cartilage injury by confocal microscopy in an ex vivo model
7. Active role of chondrocyte apoptosis in endochondral ossification
8. Apoptotic cell death is not a widespread phenomenon in normal aging and osteoarthritis human articular knee cartilage: A study of proliferation, programmed cell death (apoptosis), and viability of chondrocytes in osteoarthritic human knee cartilage
9. FrzB-2: A human secreted frizzled-related protein with a potential role in chondrocyte apoptosis
10. Effect of hyaluronan on chondrocyte apoptosis and nitric oxide production in experimentally induced osteoarthritis
11. Apoptosis of articular chondrocytes in rheumatoid arthritis and osteoarthritis: Correlation of apoptosis with degree of cartilage destruction and expression of apoptosis-related proteins of p53 and c-myc
12. Injurious mechanical compression of bovine articular cartilage induces chondrocyte apoptosis
13. Chondrocyte necrosis and apoptosis in impact damaged articular cartilage
14. Relationship between chondrocyte apoptosis and matrix depletion in human articular cartilage
15. Extracellular phosphate ions cause apoptosis of terminally differentiated epiphyseal chondrocytes
16. Vitamin C induced apoptosis in human articular chondrocytes
17. Apoptosis: Identification of dying cells
18. Apoptosis, oncosis, and necrosis: An overview of cell death
19. Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics
20. The mitochondrial death/life regulator in apoptosis and necrosis
21. Mitochondrial control of apoptosis: An overview
22. Mitochondria as the central control point of apoptosis
23. Biochemical pathways of caspase activation during apoptosis
24. Quantification of laser-induced cartilage injury by confocal microscopy in an ex vivo model
25. Activation and cleavage of caspase-3 in apoptosis induced by experimental cerebral ischemia
26. Impairment of mitochondrial respiration after cerebral hypoxia-ischemia in immature rats: Relationship to activation of caspase-3 and neuronal injury
27. Cytoskeletal disruption accelerates caspase-3 activation and alters the intracellular membrane reorganization in DNA damage-induced apoptosis
28. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis
29. A cautionary note on the use of the TUNEL stain to determine apoptosis
30. In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: A cautionary note
31. Most thymocytes die in the absence of DNA fragmentation
32. Pitfalls in the evaluation of apoptosis using TUNEL
33. Identification of necrotic cell death by the TUNEL assay in the hypoxic-ischemic neonatal rat brain
34. Physiological cell death of chondrocytes in vivo is not confined to apoptosis
35. Chromatin condensation during apoptosis requires ATP
36. Morphology of cartilage
37. Mechanisms of chondrocyte apoptosis
38. A novel rat osteoarthrosis model to assess apoptosis and matrix degradation