Identification of necrotic cell death by the TUNEL assay in the hypoxic-ischemic neonatal rat brain

Neuroscience Letters

Volumn 230, Issue 1, 1997, Pages 1-4

  De Torres, Carmen ^a   Munell, Francina ^a   Ferrer, Isidre ^c   Reventós, Jaume ^a   Macaya, Alfons ^a,b  

^a Ctr d'Investigacions en B   (Spain)

^b HOSPITAL MATERNO INFANTIL VALL D HEBRON   (Spain)

^c HOSPITAL UNIVERSITARI DE BELLVITGE   (Spain)

Author keywords

Apoptosis; Brain; DNA fragmentation; Hypoxia ischemia; Infant rat; Necrosis; TUNEL assay

Indexed keywords

DNA FRAGMENT;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BRAIN REGION; CONTROLLED STUDY; HISTOLOGY; HYPOXIA; ISCHEMIA; NEUROSIS; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; ANIMALS, NEWBORN; BRAIN; BRAIN ISCHEMIA; CELL NUCLEUS; CHROMATIN; DNA FRAGMENTATION; FUNCTIONAL LATERALITY; HYPOXIA, BRAIN; NECROSIS; RATS; RATS, SPRAGUE-DAWLEY; TIME FACTORS;

EID: 0031564831     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(97)00445-X     Document Type: Article

References (20)

1. Beilharz E.J., Williams C.E., Dragunow M., Sirimanne E.S., Gluckman P.D. Mechanisms of delayed cell death following hypoxic-ischemic injury in the immature rat: evidence for apoptosis during selective neuronal loss. Mol. Brain Res. 29:1995;1-14.
2. Cohen G.M., Sun X.M., Snowden R.T., Dinsdale D., Skilleter D.N. Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochem. J. 286:1992;311-334.
3. Ferrer I., Tortosa A., Macaya A., Sierra A., Moreno D., Munell F., Blanco R., Squier W. Evidence of nuclear DNA fragmentation following hypoxia-ischemia in the infant rat brain, and transient forebrain ischemia in the adult gerbil. Brain Pathol. 4:1994;115-122.
4. Gavrieli Y., Sherman Y., Ben-Sasson S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:1992;493-501.
5. Grasl-Kraupp B., Ruttkay-Nedecky B., Koudelka H., Burowska K., Bursch W., Schulte-Hermann R. In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: a cautionary note. Hepatology. 21:1995;1465-1468.
6. Hill I.E., MacManus J.P., Rasquinha I., Tuor U.I. DNA fragmentation indicative of apoptosis following unilateral cerebral hypoxia-ischemia. Brain Res. 676:1995;398-403.
7. Li Y., Sharov V.G., Jiang N., Zaloga C., Sabbah H.N., Chopp M. Ultrastructural and light microscopic evidence of apoptosis after middle cerebral artery occlusion in the rat. Am. J. Pathol. 146:1995;1045-1051.
8. MacManus J.P., Hill I.E., Huang Z.G., Rasquinha I., Xue D., Buchan A.M. DNA damage consistent with apoptosis in transient focal ischaemic neocortex. NeuroReport. 5:1994;493-496.
9. Majno G., Joris I. Apoptosis, oncosis, and necrosis, An overview of cell death. Am. J. Pathol. 146:1995;3-15.
10. Oberhammer, F., Fritsch, G., Pavelka, M., Froschl, G., Tiefenbacher, R., Purchio, T. and Schulte-Hermann, R., Induction of apoptosis in cultured hepatocytes and in the regressing liver by transforming growth factor beta-l occurs without activation of an endonuclease, Toxicol. Lett. 64-65 (1992) 701-714.
11. Oo T.F., Henchcliffe C., Burke R.E. Apoptosis in substantia nigra following developmental hypoxic-ischemic injury. Neuroscience. 69:1996;893-901.
12. Paxinos, G., Törk, I., Tecott, L. and Valentino, K.L., Atlas of the Developing Rat Brain, Academic Press, San Diego, CA, 1991.
13. Portera-Cailliau C., Price D.L., Martin L.J. Excitotoxic neuronal death in the immature brain is an apoptosis-necrosis morphological continuum. J. Comp. Neurol. 378:1997;70-87.
14. Rice J.E., Vannucci R.C., Brierly J.B. The influence of immaturity on hypoxic-ischemic brain damage in the rat. Ann. Neurol. 9:1981;131-141.
15. Schwartz L.M., Smith S.W., Jones M.E.E., Osborne B.A. Do all programmed cell deaths occur via apoptosis? Proc. Natl. Acad. Sci. USA. 90:1993;980-984.
16. Tominaga T., Kure S., Narisawa K., Yoshimoto T. Endonuclease activation following focal ischemic injury in the rat brain. Brain Res. 608:1993;21-26.
17. Towfighi J., Zec N., Yager J., Housman C., Vannucci R.C. Temporal evolution of neuropathologic changes in an immature rat model of cerebral hypoxia: a light microscopic study. Acta Neuropathol. 90:1995;375-386.
18. Van Lookeren Campagne M., Gill R. Ultrastructural morphological changes are not characteristic of apoptotic cell death following cerebral ischemia in the rat. Neurosci. Lett. 213:1996;111-114.
19. Yasuda M., Umemura S., Osamura R.Y., Kenjo T., Tsutsumi Y. Apoptotic cells in the human endometrium and placental villi: pitfalls in applying the TUNEL method. Arch. Histol. Cytol. 58:1995;185-190.
20. Wisjman J.H., Jonker R.R., Keijzer R., Ven de Velde C.J.H., Cornelisse C.J., Van Dierendonck J.H. A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA. J. Histochem. Cytochem. 41:1993;7-12.