Cellular specificity of proexendin-4 processing in mammalian cells in Vitro and in Vivo

Endocrinology

Volumn 143, Issue 9, 2002, Pages 3464-3471

  Adatia, F A ^a   Baggio, L L ^b   Xiao, Q ^a   Drucker, D J ^a,b   Brubaker, P L ^a,c  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c Linco Research Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EXENDIN 4; GLUCAGON LIKE PEPTIDE 1; METALLOTHIONEIN; PROEXENDIN 4; UNCLASSIFIED DRUG;

ADRENAL GLAND; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL SPECIFICITY; CONSENSUS SEQUENCE; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOHISTOCHEMISTRY; MAMMAL CELL; MOUSE; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PROCESSING; RADIOIMMUNOASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; SEQUENCE ANALYSIS; TESTIS; TRANSGENE;

ADRENOCORTICOTROPIC HORMONE; ANIMALS; ANTIBODY SPECIFICITY; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; FEMALE; FIBROBLASTS; GLUCAGON; GLUCAGON-LIKE PEPTIDE 1; IMMUNOHISTOCHEMISTRY; ISLETS OF LANGERHANS; KIDNEY; MALE; MICE; MICE, TRANSGENIC; ORGAN SPECIFICITY; PEPTIDE FRAGMENTS; PEPTIDES; PITUITARY GLAND; PROTEIN PRECURSORS; RADIOIMMUNOASSAY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFECTION; VENOMS;

EID: 0036721566     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/en.2002-220251     Document Type: Article

References (47)

1. Glucagon-like peptides
2. The glucagon-like peptides
3. Insulinotropic glucagon-like peptide 1 agonists stimulate expression of homeodomain protein IDX-1 and increase islet size in mouse pancreas
4. Exendin-4 stimulates both β-cell replication and neogenesis, resulting in increased β-cell mass and improved glucose tolerance in diabetic rats
5. Gastric emptying, glucose responses, and insulin secretion after a liquid test meal: Effects of exogenous glucagon-like peptide-1 (GLP-1)-(7-36) amide in type 2 (noninsulin-dependent) diabetic patients
6. Effects of subcutaneous glucagon-like peptide 1 (GLP-1 [7-36 amide]) in patients with NIDDM
7. Near-normalisation of diurnal glucose concentrations by continuous administration of glucagon-like peptide-1 (GLP-1) in subjects with NIDDM
8. Investigation of glucose-dependent insulinotropic polypeptide-(1-42) and glucagon-like peptide-1-(7-36) degradation in vitro by dipeptidyl peptidase IV using matrix-assisted laser desorption/ionization time of flight mass spectrometry: A novel kinetic approach
9. 2-terminus in type II diabetic patients and in healthy subjects
10. Long-lasting antidiabetic effect of a dipeptidyl peptidase IV-resistant analog of glucagon-like peptide-1
11. Biological activities of glucagon-like peptide-1 analogs in vitro and in vivo
12. Dipeptidyl peptidase IV resistant analogues of glucagon-like peptide-1 which have extended metabolic stability and improved biological activity
13. Isolation of exendin-4, an analog of exendin-3, from heloderma susceptum venom
14. Exendin-4 is a high potency agonist and truncated exendin-(9-39)-amide an antagonist at the glucagon-like peptide 1-(7-36)-amide receptor of insulin-secreting beta-cells
15. Once daily injection of exendin-4 to diabetic mice achieves long-term beneficial effects on blood glucose concentrations
16. Glucose-lowering and insulin-sensitizing actions of exendin-4: Studies in obese diabetic (ob/ob, db/db) mice, diabetic fatty Zucker rats, and diabetic rhesus monkeys (Macaca mulatta)
17. Glucagon-like peptide-1 and exendin-4 stimulate β-cell neogenesis in streptozotocin-treated newborn rats resulting in persistently improved glucose homeostasis at adult age
18. Exendin-4 reduces fasting and postprandial glucose and decreases energy intake in healthy volunteers
19. Role of prohormone convertases in the tissue-specific processing of proglucagon
20. Proglucagon processing in an islet cell line: Effects of PC1 overexpression and PC2 depletion
21. In vitro processing of mouse proglucagon by recombinant PC1 and PC2
22. Differential processing of proglucagon by the subtilisin-like prohormone convertases PC2 and PC3 to generate either glucagon or glucagon-like peptide
23. The family of subtilisin/kexin like pro-protein and pro-hormone convertases: Divergent or shared functions
24. Proteolytic processing mechanisms in the biosynthesis of neuroendocrine peptides: The subtilisin-like proprotein convertases
25. Tissue specific expression of unique mRNAs that encode proglucagon-derived peptides or exendin 4 in the lizard
26. Encapsulated, genetically engineered cells, secreting glucagon-like peptide-1 for the treatment of non-insulin-dependent diabetes mellitus
27. Sustained expression of exendin-4 does not perturb food intake, glucose homeostasis or beta cell mass in metallothionein-preproexendin transgenic mice
28. Fetal rat intestinal cells in monolayer culture: A new in vitro system to study the glucagon-related peptides
29. Circulating and tissue forms of the intestinal growth factor, glucagon-like peptide-2
30. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
31. Biosynthesis of the prohormone convertase mPC1 in AtT-20 cells
32. Cell-specific post-translational processing of preproglucagon expressed from a metallothionein-glucagon fusion gene
33. Gonadotroph-specific expression of metallothionein fusion genes in pituitaries of transgenic mice
34. Cryptic human growth hormone gene sequences direct gonadotroph-specific expression in transgenic mice
35. Overexpression of mouse follistatin causes reproductive defects in transgenic mice
36. Testicular wild-type p53 expression in transgenic mice induces spermiogenesis alterations ranging from differentiation defects to apoptosis
37. Reproductive deficiencies in transgenic mice expressing the rat inhibin α-subunit gene
38. Testicular expression of PC4 in the rat: Molecular diversity of a novel germ cell-specific Kex2/subtilisin-like proprotein convertase
39. Impaired fertility in mice deficient for the testicular germ-cell protease PC4
40. Pituitary adenylate cyclase-activating polypeptide precursor is processed solely by prohormone convertase 4 in the gonads
41. Gene expression of proprotein convertases in individual rat anterior pituitary cells and their regulation in corticotrophs mediated by glucocorticoids
42. Tissue-specific posttranslational processing of pre-prosomatostatin encoded by a metallothionein-somatostatin fusion gene in transgenic mice
43. Somatostatin is targeted to the regulated secretory pathway of gonadotrophs in transgenic mice expressing a metallothionein-somatostatin gene
44. 2)
45. Defective prohormone processing and altered pancreatic islet morphology in mice lacking active SPC2
46. Synthesis and secretion of glucagon-like peptide-1 by fetal rat intestinal cells in culture