Proglucagon processing in an islet cell line: Effects of PC1 overexpression and PC2 depletion

Endocrinology

Volumn 139, Issue 4, 1998, Pages 1630-1637

  Dhanvantari, Savita ^a   Brubaker, Patricia L ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

FURIN; GLICENTIN; GLUCAGON LIKE PEPTIDE; GLUCAGON LIKE PEPTIDE 1 [7-36] AMIDE; MESSENGER RNA; OXYNTOMODULIN; PROGLUCAGON;

ARTICLE; CELL LINE; ENZYME ACTIVITY; GENE DELETION; GENE OVEREXPRESSION; GENETIC TRANSFECTION; GLUCAGON RELEASE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HORMONE SYNTHESIS; PANCREAS ISLET CELL; PRIORITY JOURNAL; PROTEIN PROCESSING; RADIOIMMUNOASSAY; TISSUE SPECIFICITY;

EID: 0031765121     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/endo.139.4.5936     Document Type: Article

References (36)

1. Gutniak M, Orskov C, Holst JJ, Ahrén B, Efendic S 1992 Antidiabetogenic effect of glucagon-like peptide-1 (7-36)amide in normal subjects and patients with diabetes mellitus. N Engl J Med 326:1316-1322
2. Nathan DM, Schreiber E, Fogel H, Mojsov S, Habener JF 1992 Insulinotropic action of glucagonlike peptide-I-(7-37) in diabetic and nondiabetic subjects. Diabetes Care 15:270-276
3. Dubrasquet M, Bataille D, Gespach C 1982 Oxyntomodulin (glucagon-37 or bioactive enteroglucagon): a potent inhibitor of pentagastrin-stimulated acid secretion in rats. Biosci Rep 2:391-395
4. Jarrousse C, Audousset-Puech M-P, Dubrasquet M, Niel H, Martinez J, Bataille D 1985 Oxyntomodulin (glucagon-37) and its C-terminal octapeptide inhibit gastric acid secretion. FEBS Lett 188:81-84
5. Drucker DJ, Ehrlich P, Asa SL, Brubaker PL 1996 Induction of intestinal epithelial proliferation by glucagon-like peptide 2. Proc Natl Acad Sci USA 93:7911-7916
6. Brubaker PL, Izzo A, Hill M, Drucker DJ 1997 Intestinal function in mice with small bowel growth induced by glucagon-like peptide-2. Am J Physiol 272:E1050-E1058
7. Zhou A, Bloomquist BT, Mains RE 1993 The prohormone convertases PC1 and PC2 mediate distinct endoproteolytic cleavages in a strict temporal order during proopiomelanocortin biosynthetic processing. J Biol Chem 268:1763-1769
8. Smeekens SP, Montag AG, Thomas G, Albiges-Rizo C, Carroll R, Benig M, Phillips LA, Martin S, Ohagi S, Gardner P, Swift HH, Steiner DF 1992 Proinsulin processing by the subtilisin-related proprotein convertases furin, PC2, and PC3. Proc Natl Acad Sci USA 89:8822-8826
9. Brakch N, Galanopoulou AS, Patel YC, Boileau G, Seidah NG 1995 Comparative proteolytic processing of rat prosomatostatin by the convertases PC1, PC2, furin, PACE4 and PC5 in constitutive and regulated secretory pathways. FEBS Lett 362:143-146
10. Dhanvantari S, Seidah NG, Brubaker PL 1996 Role of prohormone convertases in the tissue-specific processing of proglucagon. Mol Endocrinol 10:342-355
11. Rothenberg ME, Eilertson CD, Klein K, Zhou Y, Lindberg I, McDonald JK, Mackin RB, Noe BD 1995 Processing of mouse proglucagon by recombinant prohormone convertase 1 and immunopurified prohormone convertase 2 in vitro. J Biol Chem 270:10136-10146
12. Rothenberg ME, Eilertson CD, Klein K, Mackin RB, Noe BD 1996 Evidence for redundancy in propeptide prohormone convertase activities in processing proglucagon: an antisense study. Mol Endocrinol 10:331-341
13. Rouillé Y, Martin S, Steiner DF 1995 Differential processing of proglucagon by the subtilisin-like prohormone convertases PC2 and PC3 to generate either glucagon or glucagon-like peptide. J Biol Chem 270:26488-26496
14. Scopsi L, Gullo M, Rilke F, Martin S, Steiner DF 1995 Proprotein convertases (PC1/PC3 and PC2) in normal and neoplastic human tissues: their use as markers of neuroendocrine differentiation. J Clin Endocrinol Metab 80:294-301
15. Nagamune H, Muramatsu K, Akamatsu T, Tamai Y, Izumi K, Tsuji A, Matsuda Y 1995 Distribution of the Kexin family proteases in pancreatic islets: PACE4C is specifically expressed in B cells of pancreatic islets. Endocrinology 136:357-360
16. Malide D, Seidah NG, Chrétien M, Bendayan M 1995 Electron microscopic immunocytochemical evidence for the involvement of the convertases PC1 and PC2 in the processing of proinsulin in pancreatic β-cells. J Histochem Cytochem 43:11-19
17. Rouillé Y, Westermark G, Martin SK, Steiner DF 1994 Proglucagon is processed to glucagon by prohormone convertase PC2 in αTC1-6 cells. Proc Natl Acad Sci USA 91:3242-3246
18. Takaki R, Ono J, Nakamura M, Yokogawa Y, Kumae S, Hiraoka T, Yamaguchi K, Hamaguchi K, Uchida S 1986 Isolation of glucagon-secreting cell lines by cloning insulinoma cells. In Vitro Cell Dev Biol 22:120-126
19. Ehrlich P, Tucker D, Asa SL, Brubaker PL, Drucker DJ 1994 Inhibition of pancreatic proglucagon gene expression in mice bearing subcutaneous endocrine tumors. Am J Physiol 267:E662-E671
20. Drucker DJ, Philippe J, Mojsov S 1988 Proglucagon gene expression and posttranslational processing in a hamster islet cell line. Endocrinology 123:1861-1867
21. Tucker JD, Dhanvantari S, Brubaker PL 1996 Proglucagon processing in islet and intestinal cell lines. Regul Pept 62:29-35
22. Sambrook J, Fritsch EF, Maniatis T 1989 Molecular Cloning: A Laboratory Manual, ed 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
23. Brubaker PL, Crivici A, Izzo A, Ehrlich P, Tsai CH, Drucker DJ 1997 Circulating and tissue forms of the intestinal growth factor, glucagon-like peptide-2. Endocrinology 138:4837-4843
24. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ 1951 Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275
25. Mineo I, Matsumura T, Shingu R, Namba M, Kuwajima M, Matsuzawa Y 1995 The role of prohormone convertases PC1 (PC3) and PC2 in the cell-specific processing of proglucagon. Biochem Biophys Res Commun 207:646-651
26. Marcinkiewicz M, Ramla D, Seidah NG, Chrétien M 1994 Developmental expression of the prohormone convertases PC1 and PC2 in mouse pancreatic islets. Endocrinology 135:1651-1660
27. Neerman-Arbez M, Cirulli V, Halban PA 1994 Levels of the conversion endoproteases PC1 (PC3) and PC2 distinguish between insulin-producing pancreatic islet βcells and non-β cells. Biochem J 300:57-61
28. Thomas L, Leduc R, Thorne BA, Smeekens SP, Steiner DF, Thomas G 1991 Kex2-like endoproteases PC2 and PC3 accurately cleave a model prohormone in mammalian cells: evidence for a common core of neuroendocrine processing enzymes. Proc Natl Acad Sci USA 88:5297-5301
29. Seidah NG, Benjannet S, Pareek S, Savaria D, Hamelin J, Goulet B, Laliberté J, Lazure C, Chrétien M, Murphy RA 1996 Cellular processing of the nerve growth factor precursor by the mammalian pro-protein convertases. Biochem J 314:951-960
30. Yoon JY, Beinfeld MC 1995 Expression of antisense PC1 in stably transfected RIN5F cells significantly reduces CCK 8 biosynthesis. Regul Pept 59:221-227
31. Paquet L, Massie B, Mains RE 1996 Proneuropeptide Y processing in large dense-core vesicles: manipulation of prohormone convertase expression in sympathetic neurons using adenoviruses. J Neurosci 16:964-973
32. Johanning K, Mathis JP, Lindberg I 1996 Role of PC2 in proenkephalin processing: antisense and overexpression studies. J Neurochem 66:898-907
33. Yoon JY, Beinfeld MC 1997 Prohormone convertase 1 is necessary for the formation of cholecystokinin 8 in Rin5F and STC-1 cells. J Biol Chem 272:9450-9456
34. Friedman TC, Cool DR, Jayasvasti V, Louie D, Loh YP 1996 Processing of pro-opiomelanocortin in GH3 cells: inhibition by prohormone convertase 2 (PC2) antisense mRNA. Mol Cell Endocrinol 116:89-96
35. Furuta M, Yano H, Zhou A, Rouillé Y, Holst JJ, Carroll R, Ravazzola M, Orci L, Furata H, Steiner DF 1997 Defective prohormone processing and altered pancreatic islet morphology in mice lacking active SPC2. Proc Natl Acad Sci USA 94:6646-6651
36. De Bie I, Marcinkiewicz M, Malide D, Lazure C, Nakayama K, Bendayan M, Seidah NG 1996 The isoforms of proprotein convertase PC5 are sorted to different subcellular compartments. J Cell Biol 135:1261-1275