Flow cytometric quantification of competitive reverse transcription-PCR products

Clinical Chemistry

Volumn 48, Issue 9, 2002, Pages 1398-1405

  Wedemeyer, Niels ^a   Pötter, Thomas ^a   Wetzlich, Steffi ^a   Göhde, Wolfgang ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

2,4 DINITROPHENOL; ANTIBODY; DIGOXIGENIN; INTERLEUKIN 8; MESSENGER RNA;

ANTIBODY LABELING; ARTICLE; AUTOMATION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; GENE AMPLIFICATION; GENE TARGETING; GENETIC TRANSCRIPTION; HUMAN; HYBRIDIZATION; INTERMETHOD COMPARISON; KINETICS; LYMPHOCYTE; PROTEIN SECRETION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SEQUENCE; SEQUENCE ANALYSIS; X IRRADIATION;

EID: 0036720986     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/48.9.1398     Document Type: Article

References (33)

1. Gene expression in normal and disease states - Identification of therapeutic targets
2. Quantitative RT-PCR: Pitfalls and potential
3. Determining the specificity of quantitative variation in gene expression
4. Quantitation of mRNA by the polymerase chain reaction
5. Analysis of cytokine mRNA and DNA: Detection and quantitation by competitive polymerase chain reaction
6. Quantification of HIV-1 using multiple quantitative polymerase chain reaction standards and bioluminometric detection
7. High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR
8. Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay
9. Comparative study of a modified competitive RT-PCR and Amplicor HCV monitor assays for quantitation of hepatitis C virus RNA in Serum
10. Competitive reverse transcription/polymerase chain reaction for the quantification of p53 and mdm2 mRNA expression
11. Steady-state regulation of the human DNA mismatch repair system
12. Genotype determination at the survival motor neuron locus in a normal population and SMA carriers using competitive PCR and primer extension
13. Quantification of minimal residual disease in patients with BCR-ABL-positive acute lymphoblastic leukaemia using quantitative Competitive polymerase chain reaction
14. General colorimetric method for DNA diagnostics allowing direct solid-phase genomic sequencing of the positive samples
15. A novel method for real time quantitative RT-PCR
16. Flow cytometric analysis of reverse transcription-PCR products: Quantification of p21(WAF1/CIP1) and proliferating cell nuclear antigen mRNA
17. Triple primer polymerase chain reaction. A new way to quantify truncated mRNA expression
18. Interleukin-8 induces rapid mobilization of hematopoietic stem cells with radioprotective capacity and long-term myelolymphoid repopulating ability
19. Technical aspects of quantitative competitive PCR
20. Reproducibility in the quantification of mRNA levels by RT-PCR-ELISA and RT competitive-PCR-ELISA
21. Quantitative analysis of gene expression by ion-pair high-performance liquid chromatography
22. High-resolution capillary electrophoretic analysis of DNA in free solution
23. Quantification of HER2/neu gene amplification by competitive PCR using fluorescent melting curve analysis
24. Regulation of cytokine gene expression by reactive oxygen and reactive nitrogen intermediates
25. Modulation of radiation-induced cytokine elevation associated with esophagitis and esophageal stricture by manganese superoxide, dismutase-plasmid/liposome (SOD2-PL) gene therapy
26. Rapid isolation of PCR-ready DNA from blood, bone marrow and cultured cells, based on paramagnetic beads
27. Magnetic, microplate-format Plasmid isolation protocol for high-yield, sequencing-grade DNA
28. Flow cytometry: An 'old' tool for novel applications in medical genetics
29. Direct quantification of HIV by flow cytometry using branched DNA signal amplification
30. Flow cytometry-based minisequencing: A new platform for high-throughput single-nucleotide polymorphism scoring
31. A microsphere-based assay for multiplexed single nucleotide polymorphism analysis using single base chain extension
32. HTPS flow cytometry: A novel platform for automated high throughput drug discovery and characterization
33. Advanced multiplexed analysis with the FlowMetrix system