Direct quantitation of HIV by flow cytometry using branched DNA signal amplification

Molecular and Cellular Probes

Volumn 12, Issue 4, 1998, Pages 243-247

  Van Cleve, M ^a   Ostrerova, N ^a,c   Tietgen, K ^a,d   Cao, W ^b   Chang, C ^b   Collins, M L ^b,e   Kolberg, J ^b   Urdea, M ^b   Lohman, K ^a,f  

^a BD Technologies   (United States)

^b NOVARTIS PHARMA AG   (Switzerland)

^c LOYOLA UNIVERSITY CHICAGO   (United States)

^d Evotec Technologies GmbH   (Germany)

^e Nanogen Inc   (United States)

^f APPLIED BIOSYSTEMS   (United States)

Author keywords

bDNA; Flow cytometry; Plasma viremia

Indexed keywords

DNA; RNA;

ARTICLE; ASSAY; DNA DETERMINATION; FLOW CYTOMETRY; GENE AMPLIFICATION; HUMAN IMMUNODEFICIENCY VIRUS; INTERMETHOD COMPARISON; LYSIS; NONHUMAN; NUCLEIC ACID PROBE; PRIORITY JOURNAL; VIRUS DETECTION;

EID: 0032142761     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1998.0179     Document Type: Article

References (6)

1. Pachl C, Todd J. A, Kern D. G. Rapid and precise quantification of HIV-1 RNA in plasma using a branched DNA signal amplification assay. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 8:1995;446-454.
2. Collins M, Irvine B, Tyner D. A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml. Nucleic Acids Research. 25:(1997);2979-2985.
3. Sanchez-Pescador R, Power M. D, Barr P. J. Nucleotide sequence and expression of an AIDS-associated retrovirus (ARV-2). Science. 227:1985;484-492.
4. Urdea M. S, Horn T, Fultz T. J. Branched DNA amplification multimers for the sensitive, direct detection of human hepatitis viruses. Nucleic Acids Research Symposium. Ser 24:1991;197-200.
5. Shapiro Howard M. Practical Flow Cytometry. 1988.
6. Verhofstede C, Fransen K, Marissens D. Isolation of HIV-1 RNA from plasma: evaluation of eight different extraction methods. Journal of Virological Methods. 60:1996;155-159.