Flow cytometry: An 'old' tool for novel applications in medical genetics

Clinical Genetics

Volumn 60, Issue 1, 2001, Pages 1-8

  Wedemeyer, N ^a   Potter T ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Flow cytometry; Microspheres; Quantitative reverse transcription polymerase chain reaction (RT PCR); Single nucleotide polymorphism (SNP); Sorting; Viral load

Indexed keywords

DNA; MICROSPHERE; NUCLEOTIDE; VIRUS DNA;

BIOMEDICAL TECHNOLOGY ASSESSMENT; CELL CYCLE; CELL SUSPENSION; CLINICAL LABORATORY; CONTROLLED STUDY; DNA CONTENT; DNA DETERMINATION; DNA PROBE; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; GENE EXPRESSION; GENE SEQUENCE; GENETIC POLYMORPHISM; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; LABORATORY AUTOMATION; MEDICAL GENETICS; MOLECULAR DYNAMICS; NONHUMAN; OPTICAL INSTRUMENTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; REVIEW; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; VIRAL GENETICS;

DNA; FLOW CYTOMETRY; GENETICS, MEDICAL; HUMANS; POLYMORPHISM, SINGLE NUCLEOTIDE;

EID: 0034918708     PISSN: 00099163     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-0004.2001.600101.x     Document Type: Review

References (60)

1. Flow cytometry: Principles and clinical applications in hematology
2. Pulse cytophotometry of DNA in skin tumours
3. Phagocytosis: Flow cytometric quantitation with fluorescent microspheres
4. Quantitation of cell concentration using the flow cytometer
5. Quantitative flow cytometry: Inter-laboratory variation
6. New immunolatex spheres: Visual markers of antigens on lymphocytes for scanning electron microscopy
7. A fluorescence immunoassay for soluble antigens employing flow cytometric detection
8. The effect of polyclonal antibody against fibronectin on von Willebrand factor multimeric size
9. Surface antigen detection with non-fluorescent, antibody-coated microbeads: An alternative method compatible with conventional fluorochrome-based labeling
10. Simultaneous detection of cucumber mosaic virus, tomato mosaic virus and potatovirus Y by flow cytometry
11. The sensitive detection and quantitation of antibody to HCV by using a microsphere-based immunoassay and flow cytometry
12. Flow cytometric analysis of immunoprecipitates: High-throughput analysis of protein phosphorylation and protein-protein interactions
13. The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions
14. Application of molecular biology-based methods to the diagnosis of infectious diseases
15. AIDS update: Viral reservoirs and immune-based therapies
16. Molecular methods of measurement of hepatitis B virus, hepatitis C virus, and human immunodeficiency virus infection: Implications for occupational health practice
17. HIV-1 viral load: Comparative evaluation of three commercially available assays in Argentina
18. Detection of Hepatitis B virus in plasma using flow cytometric analyses of polymerase chain reaction-amplified DNA incorporating digoxigenin-11-dUTP
19. Flow cytometric detection of human immunodeficiency virus type 1 proviral DNA by the polymerase chain reaction incorporating digoxigenin- or fluorescein-labeled dUTP
20. Quantification of polymerase chain reaction products by hybridization-based assays with fluorescent, colorimetric, or chemiluminescent detection
21. Transmission of HIV-1 in infants born to seropositive mothers: PCR-amplified proviral DNA detected by flow cytometric analysis of immunoreactive beads
22. Direct quantification of HIV by flow cytometry using branched DNA signal amplification
23. A rapid high-throughput method for DNA-based pathogen detection
24. Simultaneous detection of multiplex-amplified human immunodeficiency virus type 1 RNA, hepatitis C virus RNA, and hepatitis B virus DNA using a flow cytometer microsphere-based hybridization assay
25. A rapid, sensitive, multiplexed assay for detection of viral nucleic acids using the FlowMetrix system
26. Expression genetics: A different approach to cancer diagnosis and prognosis
27. Use of a cDNA microarray to analyse gene expression patterns in human cancer
28. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction
29. Serial analysis of gene expression
30. In vitro cloning of complex mixtures of DNA on microbeads: Physical separation of differentially expressed cDNAs
31. Oligonucleotide-conjugated beads for transdominant genetic experiments
32. Molecular pathology and future developments
33. Quantitative RT-PCR: Pitfalls and potential
34. Analysis of cytokine mRNA and DNA: Detection and quantitation by competitive polymerase chain reaction
35. Rapid quantitation of gene therapy specific CFTR expression using the amplification refractory mutation system
36. A novel method for real time quantitative RT-PCR
37. Flow cytometric analysis of reverse transcriptase-PCR products: Quantitation of p21WAF1/CIP1 and proliferating cell nuclear antigen mRNA
38. The future of genetic studies of complex human diseases
39. An estimate of unique DNA sequence heterozygosity in the human genome
40. Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes
41. From gels to chips: 'Minisequencing' primer extension for analysis of point mutations and single nucleotide polymorphisms
42. A ligase-mediated gene detection technique
43. Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes
44. Multiplex, fluorescent, solid-phase minisequencing for efficient screening of DNA sequence variation
45. Single-well genotyping of diallelic sequence variations by a two-color ELISA-based oligonucleotide ligation assay
46. Single-nucleotide polymorphism analysis by MALDI-TOF mass spectrometry
47. Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome
48. Suspension arrays for high throughput, multiplexed single nucleotide polymorphism genotyping
49. Flow cytometry-based minisequencing: A new platform for high-throughput single-nucleotide polymorphism scoring
50. Multiplexed single nucleotide polymorphism genotyping by oligonucleotide ligation and flow cytometry
51. A microsphere-based assay for multiplexed single nucleotide polymorphism analysis using single base chain extension
52. Association of apolipoprotein E allele epsilon 4 with late-onset familial and sporadic Alzheimer's disease
53. Commercial high speed machines open new opportunities in high throughput flow cytometry (HTFC)
54. High-speed flow cytometric analysis and sorting of human fetal cells from maternal blood for molecular characterization
55. In vitro production of sexed embryos for gender preselection: High-speed sorting of X-chromosome-bearing sperm to produce pigs after embryo transfer
56. High-speed chromosome sorting
57. Magnetic, microplate-format plasmid isolation protocol for high-yield, sequencing-grade DNA
58. Rapid isolation of PCR-ready DNA from blood, bone marrow and cultured cells, based on paramagnetic beads
59. High-throughput extraction, amplification, and detection (HEAD) of HCV-RNA in individual blood donations
60. Advanced multiplexed analysis with the FlowMetrix system