Isolation of novel tRNAAla mutants by library selection in a tRNAAla knockout strain

Biochimie

Volumn 84, Issue 8, 2002, Pages 705-711

  Choi, H ^a   Otten, S ^a   McClain, W H ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Aminoacyl tRNA synthetase; Functional tRNA mutants; Knockout selection; tRNA identity

Indexed keywords

ALANINE TRANSFER RNA; AMINOACYL TRANSFER RNA; NUCLEOTIDE; TRANSFER RNA; AMINO ACID TRANSFER RNA LIGASE; OLIGORIBONUCLEOTIDE;

AMINOACYLATION; ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; CONTROLLED STUDY; ENZYME BINDING; ESCHERICHIA COLI; GENE LIBRARY; GENETIC SELECTION; KNOCKOUT GENE; MOLECULAR RECOGNITION; MOLECULAR WEIGHT; MUTANT; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; RANDOMIZATION; RNA ISOLATION; RNA SEQUENCE; RNA STRUCTURE; SITE DIRECTED MUTAGENESIS; AUDIOVISUAL EQUIPMENT; CONFORMATION; CYTOLOGY; ENZYME SPECIFICITY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; SYNTHESIS;

AMINO ACYL-TRNA SYNTHETASES; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE LIBRARY; MODELS, STRUCTURAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; OLIGORIBONUCLEOTIDES; PLASMIDS; RNA, TRANSFER, ALA; SUBSTRATE SPECIFICITY;

EID: 0036703183     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-9084(02)01407-4     Document Type: Article

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