Functional evidence for indirect recognition of G·U in tRNAAla by alanyl-tRNA synthetase

Science

Volumn 271, Issue 5246, 1996, Pages 195-197

  Gabriel, K ^a   Schneider, Jay ^a   McClain, William H ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE TRANSFER RNA; BACTERIAL RNA; GUANINE; TRANSFER RNA; URACIL;

AMINOACYLATION; ARTICLE; BACTERIAL GENE; BACTERIUM MUTANT; ESCHERICHIA COLI; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; PROTEIN RNA BINDING; RNA STRUCTURE;

ALANINE-TRNA LIGASE; ANTICODON; BASE COMPOSITION; BASE SEQUENCE; ESCHERICHIA COLI; GENES, BACTERIAL; GUANINE; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEIC ACID CONFORMATION; PLASMIDS; RNA, TRANSFER, ALA; URACIL;

EID: 0030023881     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5246.195     Document Type: Article

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23. The chromosome sequence was from the EcoSeq database, locus alaWecoM, accession number ES1002. The primer sequences used in Fig. 2 were as follows: P1, 5′-GGGAAATCAAAGAAGCTCCC-3′; P2, 5′-GGCACGACCGCCTTTCACGC-3′; P3, 5′-GTCAGCAACACCTTCTTCAC-3′; P4, 5′-CTTCAGCTTCCATCAACATT-3′; P5, 5′-AAGCGGCAAAAAGCAGAGAC-3′; and P6, 5′-GCAATTTGCCGTTGACACAT-3′.
24. R gene from pBR322, and a synthetic ala2 gene from pGFIB
25. We thank G Bjork, L. Kiessling, G Varani, and M Yams for cogent discussions and S Artz, H. Inokuchi, S Kushner, J Miller, and C. Squires for materials. Supported by U S Public Health Service grant GM42123.