A new in vitro model to evaluate differential responses of endothelial cells to simulated arterial shear stress waveforms

Journal of Biomechanical Engineering

Volumn 124, Issue 4, 2002, Pages 397-407

  Blackman, Brett R ^a   García Cardeña, Guillermo ^a   Gimbrone Jr , Michael A ^a  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

Blood Flow; eNOS; Mechanotransduction; Migration

Indexed keywords

BLOOD; CELLS; HEMODYNAMICS; PROTEINS; RNA; SHEAR STRESS; WAVEFORM ANALYSIS;

SHEARING FORCES;

BIOMEDICAL ENGINEERING;

ACTIN; MESSENGER RNA; NITRIC OXIDE SYNTHASE; PROTEIN; VINCULIN;

ARTICLE; BLOOD FLOW; CELL MIGRATION; ENDOTHELIUM CELL; FLOW MEASUREMENT; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMAGE ANALYSIS; IMMUNOBLOTTING; PROTEIN ISOLATION; PULSATILE FLOW; SHEAR STRESS; SIMULATION; WAVEFORM; ADAPTATION; ARTERY; BLOOD FLOW VELOCITY; BLOOD RHEOLOGY; CELL CULTURE; CELL MOTION; CYTOLOGY; EVALUATION; FLOW CYTOMETRY; IN VITRO STUDY; INSTRUMENTATION; MECHANICAL STRESS; MECHANOTRANSDUCTION; METHODOLOGY; PHYSIOLOGY; UMBILICAL VEIN; VALIDATION STUDY; VASCULAR ENDOTHELIUM;

ADAPTATION, PHYSIOLOGICAL; ARTERIES; BLOOD FLOW VELOCITY; CELL MOVEMENT; CELLS, CULTURED; ENDOTHELIUM, VASCULAR; FLOW CYTOMETRY; HEMORHEOLOGY; HUMAN; IN VITRO; MECHANOTRANSDUCTION, CELLULAR; PULSATILE FLOW; STRESS, MECHANICAL; SUPPORT, U.S. GOV'T, P.H.S.; UMBILICAL VEINS; HUMANS;

EID: 0036675545     PISSN: 01480731     EISSN: None     Source Type: Journal    
DOI: 10.1115/1.1486468     Document Type: Article

References (65)

1. Flow-mediated endothelial mechanotransduction
2. Laminar shear stress: Mechanisms by which endothelial cells transduce an atheroprotective force
3. Fluid shear stress increases membrane fluidity in endothelial cells: A study with DCVJ fluorescence
4. Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix ligands
5. The dynamic response of vascular endothelial cells to fluid shear stress
6. Effects of pulsatile flow on cultured vascular endothelial cell morphology
7. Shear stress modulates endothelial cell morphology and f-actin organization through the regulation of focal adhesion-associated proteins
8. Oscillatory shear stress and hydrostatic pressure modulate cell-matrix attachment proteins in cultured endothelial cells
9. Vascular endothelium responds to fluid shear stress gradients
10. Turbulent fluid shear stress induces vascular endothelial cell turnover in vitro
11. Nitric oxide synthesis by cultured endothelial cells is modulated by flow conditions
12. Identification of vascular endothelial genes differentially responsive to fluid mechanical stimuli: Cyclooxygenase-2, manganese superoxide dismutase, and endothelial cell nitric oxide synthase are selectively up-regulated by steady laminar shear stress
13. Biomechanical activation of vascular endothelium as a determinant of its functional phenotype
14. Endothelial dysfunction, hemodynamic forces, and atherogenesis
15. A device for subjecting vascular endothelial cells to both fluid shear stress and circumferential cyclic stretch
16. In vitro system to study realistic pulsatile flow and stretch signaling in cultured vascular cells
17. Interaction between wall shear stress and circumferential strain affects endothelial cell biochemical production
18. Integrity of arterial endothelium following acute exposure to high shear stress
19. Improved in vitro rheological system for studying the effect of fluid shear stress on cultured cells
20. In vitro cell shearing device to investigate the dynamic response of cells in a controlled hydrodynamic environment
21. Human abdominal aorta: Comparative measurements of blood flow with MR imaging and multigated doppler US
22. Determination of wall shear stress in the aorta with the use of MR phase velocity mapping
23. In vivo wall shear stress measured by magnetic resonance velocity mapping in the normal human abdominal aorta
24. Nonlinear analysis of aortic flow in living dogs
25. A nonlinear analysis of pulsatile flow in arteries
26. Characterizing a kinesis response: Time averaged measures of cell speed and directional persistence
27. Pulsatile flow and atherosclerosis in the human carotid bifurcation. Positive correlation between plaque location and low oscillating shear stress
28. Fluid wall shear stress measurements in a model of the human abdominal aorta: Oscillatory behavior and relationship to atherosclerosis
29. The secondary flow of Newtonian fluids in a cone-and-plate viscometers
30. Secondary flow and turbulence in a cone-and-plate device
31. The shape of endothelial cells in en face preparations of rabbit blood vessels
32. Relationship between blood flow direction and endothelial orientation at arterial branch sites in rabbits and mice
33. Adhesion plaques: Sites of transmembrane interaction between the extracellular matrix and the actin cytoskeleton
34. Endothelial cell signaling and cytoskeletal changes in response to shear stress
35. Shear stress induces spatial reorganization of the endothelial cell cytoskeleton
36. Flow-induced release of endothelium-derived relaxing factor
37. Molecular cloning and characterization of the constitutive bovine aortic endothelial cell nitric oxide synthase
38. Role of calcium and calmodulin in flow-induced nitric oxide production in endothelial cells
39. Chronic exercise in dogs increases coronary vascular nitric oxide production and endothelial cell nitric oxide synthase gene expression
40. Shear stress regulates endothelial nitric oxide synthase expression through C-src by divergent signaling pathways
41. Calcium responses of endothelial cell monolayers subjected to pulsatile and steady laminar flow differ
42. Quantitative morphodynamics of endothelial cells within confluent cultures in response to fluid shear stress
43. Quantitative studies of endothelial cell adhesion. Directional remodeling of focal adhesion sites in response to flow forces
44. Overexpression of vinculin suppresses cell moltility in BALB/c 3T3 cells
45. Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA
46. Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton
47. Regulation of the actin cycle in vivo by actin filament severing
48. Shear stress gradients remodel endothelial monolayers in vitro via a cell proliferation-migration-loss cycle
49. Factors influencing the expression of stress fibers in vascular endothelial cells in situ
50. Actin filament stress fibers in vascular endothelial cells in vivo
51. In vivo modulation of endothelial f-actin microfilaments by experimental alterations in shear stress
52. Induction of human vascular endothelial stress fibers by fluid shear stress
53. Fibronectin and f-actin redistribution in cultured endothelial cells exposed to shear stress
54. Expression and intracellular distribution of stress fibers in aortic endothelium
55. Direct evidence for the importance of endothelium-derived nitric oxide in vascular remodeling
56. Molecular control of nitric oxide synthases in the cardiovascular system
57. Modulation by pathophysiological stimuli of the shear stress-induced up-regulation of endothelial nitric oxide synthase expression in endothelial cells
58. Shear stress regulation of endothelial NOS in fetal pulmonary arterial endothelial cells involves PKC
59. Regulation of endothelial cell nitric oxide synthase mRNA expression by shear stress
60. Influence of oscillatory and unidirectional flow environments on the expression of endothelin and nitric oxide synthase in cultured endothelial cells
61. i response to flow by the onset rate of shear stress
62. Temporal gradient in shear but not steady shear stress induces PDGF-A and MCP-1 expression in endothelial cells; Role of NO, NFkκB, and egr-l
63. Temporal gradient in shear-induced signaling pathway: Involvement of MAP kinase, c-fos, and connexin-43
64. Mechanism of temporal gradients in shear-induced ERK1/2 activation and proliferation in endothelial cells