-
1
-
-
0032694167
-
A sampling of the yeast proteome
-
Futcher B., Latter G., Monardo P., McLaughlin C., Garrels J. A sampling of the yeast proteome. Mol Cell Biol. 19:1999;7357-7368.
-
(1999)
Mol Cell Biol
, vol.19
, pp. 7357-7368
-
-
Futcher, B.1
Latter, G.2
Monardo, P.3
McLaughlin, C.4
Garrels, J.5
-
2
-
-
0034517388
-
Identification of novel MAP kinase pathway signaling targets by functional proteomics and mass spectrometry
-
Lewis T., Hunt J., Aveline L., Jonscher K., Louie D., Yeh J., Nahreini T., Resing K., Ahn N. Identification of novel MAP kinase pathway signaling targets by functional proteomics and mass spectrometry. Mol Cell. 6:2000;1343-1354. Demonstration of the global capabilities of 2DGE to monitor the activation of signal transduction pathways. In this study, 25 new substrates of the MAP kinase pathway were identified.
-
(2000)
Mol Cell
, vol.6
, pp. 1343-1354
-
-
Lewis, T.1
Hunt, J.2
Aveline, L.3
Jonscher, K.4
Louie, D.5
Yeh, J.6
Nahreini, T.7
Resing, K.8
Ahn, N.9
-
3
-
-
0035408680
-
Rapid and simple single nanogram detection of glycoproteins in polyacrylamide gels and on electroblots
-
Steinberg T., Pretty On Top K., Berggren K., Kemper C., Jones L., Diwu Z., Haugland R., Patton W. Rapid and simple single nanogram detection of glycoproteins in polyacrylamide gels and on electroblots. Proteomics. 1:2001;841-855. Demonstration of global fluorescence detection at the nanogram level of glycosylation and protein expression levels in complex samples from a single polyacrylamide gel.
-
(2001)
Proteomics
, vol.1
, pp. 841-855
-
-
Steinberg, T.1
Pretty On Top, K.2
Berggren, K.3
Kemper, C.4
Jones, L.5
Diwu, Z.6
Haugland, R.7
Patton, W.8
-
4
-
-
0034662907
-
Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology
-
Gygi S., Corthals G., Zhang Y., Rochon Y., Aebersold R. Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology. Proc Natl Acad Sci USA. 97:2000;9390-9395.
-
(2000)
Proc Natl Acad Sci USA
, vol.97
, pp. 9390-9395
-
-
Gygi, S.1
Corthals, G.2
Zhang, Y.3
Rochon, Y.4
Aebersold, R.5
-
5
-
-
0035252854
-
2D or not 2D. Two-dimensional gel electrophoresis
-
Fey S., Larson P. 2D or not 2D. Two-dimensional gel electrophoresis. Curr Opin Chem Biol. 5:2001;26-33.
-
(2001)
Curr Opin Chem Biol
, vol.5
, pp. 26-33
-
-
Fey, S.1
Larson, P.2
-
6
-
-
0005143684
-
Why proteomics can't let go of 2D gels
-
Sender A. Why proteomics can't let go of 2D gels. Genome Technol. 12:2001;34-38.
-
(2001)
Genome Technol
, vol.12
, pp. 34-38
-
-
Sender, A.1
-
7
-
-
0003225257
-
What place polyacrylamide in proteomics?
-
Herbert B., Harry J., Packer N., Gooley A., Pedersen S., Williams K. What place polyacrylamide in proteomics? Trends Biotechnol. 19:2001;S3-S9.
-
(2001)
Trends Biotechnol
, vol.19
-
-
Herbert, B.1
Harry, J.2
Packer, N.3
Gooley, A.4
Pedersen, S.5
Williams, K.6
-
8
-
-
0035207977
-
Possibilities to improve automation, speed and precision of proteome analysis: A comparison of two-dimensional electrophoresis and alternatives
-
Hille J., Freed A., Watzig H. Possibilities to improve automation, speed and precision of proteome analysis: a comparison of two-dimensional electrophoresis and alternatives. Electrophoresis. 22:2001;4035-4052.
-
(2001)
Electrophoresis
, vol.22
, pp. 4035-4052
-
-
Hille, J.1
Freed, A.2
Watzig, H.3
-
9
-
-
0036208433
-
Two-dimensional gel electrophoresis in proteomics: Old, old fashioned, but it still climbs up the mountains
-
Rabilloud T. Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains. Proteomics. 2:2002;3-10.
-
(2002)
Proteomics
, vol.2
, pp. 3-10
-
-
Rabilloud, T.1
-
10
-
-
0033027936
-
Proteome analysis II. Protein subcellular redistribution; Linking physiology to genomics via the proteome and separation technologies involved
-
Patton W. Proteome analysis II. Protein subcellular redistribution; linking physiology to genomics via the proteome and separation technologies involved. J Chromatogr B. 722:1999;203-223.
-
(1999)
J Chromatogr B
, vol.722
, pp. 203-223
-
-
Patton, W.1
-
11
-
-
0033915670
-
Background-free, high-sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex
-
Berggren K., Chernokalskaya E., Steinberg T., Kemper C., Lopez M., Diwu Z., Haugland R., Patton W. Background-free, high-sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex. Electrophoresis. 21:2000;2509-2521.
-
(2000)
Electrophoresis
, vol.21
, pp. 2509-2521
-
-
Berggren, K.1
Chernokalskaya, E.2
Steinberg, T.3
Kemper, C.4
Lopez, M.5
Diwu, Z.6
Haugland, R.7
Patton, W.8
-
12
-
-
0033667456
-
A comparison of silver stain and SYPRO ruby protein gel stain with respect to protein detection in two-dimensional gels and identification by peptide mass profiling
-
Lopez M., Berggren K., Chernokalskaya E., Lazarev A., Robinson M., Patton W. A comparison of silver stain and SYPRO ruby protein gel stain with respect to protein detection in two-dimensional gels and identification by peptide mass profiling. Electrophoresis. 21:2000;3673-3683.
-
(2000)
Electrophoresis
, vol.21
, pp. 3673-3683
-
-
Lopez, M.1
Berggren, K.2
Chernokalskaya, E.3
Lazarev, A.4
Robinson, M.5
Patton, W.6
-
13
-
-
0034075850
-
Making blind robots see; The synergy between fluorescent dyes and imaging devices in automated proteomics
-
Patton W. Making blind robots see; the synergy between fluorescent dyes and imaging devices in automated proteomics. Biotechniques. 28:2000;944-957. This paper describes integration of fluorescence detection technologies into the 2DGE-MS proteomics work environment.
-
(2000)
Biotechniques
, vol.28
, pp. 944-957
-
-
Patton, W.1
-
14
-
-
0034111635
-
A thousand points of light; The application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics
-
Patton W. A thousand points of light; the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics. Electrophoresis. 21:2000;1123-1144. This review article is a comprehensive summary of 50 years worth of fluorescence and colorimetric detection technologies for polyacrylamide gels and electroblot membranes. The paper demonstrates that fluorescence detection technologies offer a 1000-fold linear dynamic range of quantification.
-
(2000)
Electrophoresis
, vol.21
, pp. 1123-1144
-
-
Patton, W.1
-
15
-
-
0036468910
-
Rainbow's end: The quest for multiplexed fluorescence quantitative analysis in proteomics
-
Patton W., Beechem J. Rainbow's end: the quest for multiplexed fluorescence quantitative analysis in proteomics. Curr Opin Chem Biol. 6:2002;63-69. A comparison of two fluorescence-based multiplexed detection strategies suitable for 2DGE. Difference gel electrophoresis utilizes a gel-based strategy that is analogous to ICAT, whereas MP employs different fluorophores to highlight different functional attributes of proteins.
-
(2002)
Curr Opin Chem Biol
, vol.6
, pp. 63-69
-
-
Patton, W.1
Beechem, J.2
-
16
-
-
0032875697
-
Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
-
Gygi S., Rist B., Gerber S., Turecek F., Gelb M., Aebersold R. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat Biotechnol. 17:1999;994-999.
-
(1999)
Nat Biotechnol
, vol.17
, pp. 994-999
-
-
Gygi, S.1
Rist, B.2
Gerber, S.3
Turecek, F.4
Gelb, M.5
Aebersold, R.6
-
17
-
-
0033679078
-
Quantitative proteome analysis. Methods and applications
-
Aebersold R., Rist B., Gygi S. Quantitative proteome analysis. Methods and applications. Ann New York Acad Sci. 919:2000;33-47.
-
(2000)
Ann New York Acad Sci
, vol.919
, pp. 33-47
-
-
Aebersold, R.1
Rist, B.2
Gygi, S.3
-
18
-
-
0035282172
-
Quantitative proteomic analysis using a MALDI quadrupole time-of-flight mass spectrometer
-
Griffin T., Gygi S., Rist B., Aebersold R., Loboda A., Jilkine A., Ens W., Standing K. Quantitative proteomic analysis using a MALDI quadrupole time-of-flight mass spectrometer. Anal Chem. 73:2001;978-986.
-
(2001)
Anal Chem
, vol.73
, pp. 978-986
-
-
Griffin, T.1
Gygi, S.2
Rist, B.3
Aebersold, R.4
Loboda, A.5
Jilkine, A.6
Ens, W.7
Standing, K.8
-
19
-
-
0036049889
-
Quantitative protein profiling using two-dimensional gel electrophoresis, isotope coded affinity tag labeling and mass spectrometry
-
in press
-
Smolka M, Zhou H, Aebersold R: Quantitative protein profiling using two-dimensional gel electrophoresis, isotope coded affinity tag labeling and mass spectrometry. Mol Cell Proteomics 2002, in press.
-
(2002)
Mol Cell Proteomics
-
-
Smolka, M.1
Zhou, H.2
Aebersold, R.3
-
20
-
-
0035477025
-
Optimization of the isotope-coded affinity tag-labeling procedure for quantitative proteome analysis
-
Smolka M., Zhou H., Purkayastha S., Aebersold R. Optimization of the isotope-coded affinity tag-labeling procedure for quantitative proteome analysis. Anal Biochem. 297:2001;25-31.
-
(2001)
Anal Biochem
, vol.297
, pp. 25-31
-
-
Smolka, M.1
Zhou, H.2
Purkayastha, S.3
Aebersold, R.4
-
21
-
-
0002532058
-
Current trends in differential expression proteomics: Isotopically coded tags
-
Moseley M. Current trends in differential expression proteomics: isotopically coded tags. Trends Biotechnol. 19:2001;S10-S16. Detailed review of the ICAT method for protein quantification. Thiol-reactive biotin tags are utilized to label two samples, which are subsequently combined, proteolyzed, affinity fractionated and evaluated by MS.
-
(2001)
Trends Biotechnol
, vol.19
-
-
Moseley, M.1
-
22
-
-
0036161596
-
Mass spectrometry in coupling with affinity capture-release and isotope-coded affinity tags for quantitative protein analysis
-
Turecek F. Mass spectrometry in coupling with affinity capture-release and isotope-coded affinity tags for quantitative protein analysis. J Mass Spectrom. 37:2002;1-14.
-
(2002)
J Mass Spectrom
, vol.37
, pp. 1-14
-
-
Turecek, F.1
-
23
-
-
0035499083
-
Fractionation of isotopically labeled peptides in quantitative proteomics
-
Zhang R., Sioma C., Wang S., Regnier F. Fractionation of isotopically labeled peptides in quantitative proteomics. Anal Chem. 73:2001;5142-5149.
-
(2001)
Anal Chem
, vol.73
, pp. 5142-5149
-
-
Zhang, R.1
Sioma, C.2
Wang, S.3
Regnier, F.4
-
24
-
-
2142856295
-
Proteome profiling - Pitfalls and progress
-
Haynes P., Yates J. Proteome profiling - Pitfalls and progress. Yeast. 17:2000;81-87.
-
(2000)
Yeast
, vol.17
, pp. 81-87
-
-
Haynes, P.1
Yates, J.2
-
25
-
-
0036463388
-
Selective detection of membrane proteins without antibodies: A mass spectrometric version of the western blot
-
in press
-
Arnott D, Kishiyama A, Luis E, Ludlum S, Marsters J, Stults J: Selective detection of membrane proteins without antibodies: a mass spectrometric version of the western blot. Mol Cell Proteomics 2002, in press. Description of a technique for the analysis of specific proteins in complex mixtures using ICAT reagents and MS. The paper demonstrates that the dynamic range of ICAT is roughly 10-fold.
-
(2002)
Mol Cell Proteomics
-
-
Arnott, D.1
Kishiyama, A.2
Luis, E.3
Ludlum, S.4
Marsters, J.5
Stults, J.6
-
27
-
-
70449634926
-
Two-dimensional database of mouse liver proteins, an update
-
Fountoulakis M., Juranville J., Berndt P., Langen H., Suter L. Two-dimensional database of mouse liver proteins, an update. Electrophoresis. 22:2001;1747-1763.
-
(2001)
Electrophoresis
, vol.22
, pp. 1747-1763
-
-
Fountoulakis, M.1
Juranville, J.2
Berndt, P.3
Langen, H.4
Suter, L.5
-
28
-
-
0035137205
-
Role of alternative splicing in generating isoforms diversity among plasma membrane calcium pumps
-
Strehler E., Zacharias D. Role of alternative splicing in generating isoforms diversity among plasma membrane calcium pumps. Physiol Rev. 81:2001;21-50.
-
(2001)
Physiol Rev
, vol.81
, pp. 21-50
-
-
Strehler, E.1
Zacharias, D.2
-
29
-
-
0034789979
-
Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry
-
Han D., Eng J., Zhou H., Aebersold R. Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry. Nat Biotechnol. 19:2001;946-951. Comprehensive study of microsomal/mitochondrial proteins detected using the ICAT technique. It is particularly useful to compare the proteins identified in this study with proteins identified by 2DGE/MS from microsomal/mitochondrial databases.
-
(2001)
Nat Biotechnol
, vol.19
, pp. 946-951
-
-
Han, D.1
Eng, J.2
Zhou, H.3
Aebersold, R.4
-
30
-
-
0035980117
-
Proteomic analysis of the mammalian mitochondrial ribosome. Identification of protein components in the 28S small subunit
-
Suzuki T., Terasaki M., Takemoto-Hori C., Hanada T., Ueda T., Wada A., Watanabe K. Proteomic analysis of the mammalian mitochondrial ribosome. Identification of protein components in the 28S small subunit. J Biol Chem. 276:2001;33181-33195.
-
(2001)
J Biol Chem
, vol.276
, pp. 33181-33195
-
-
Suzuki, T.1
Terasaki, M.2
Takemoto-Hori, C.3
Hanada, T.4
Ueda, T.5
Wada, A.6
Watanabe, K.7
-
31
-
-
0035877697
-
Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria
-
Suzuki T., Terasaki M., Takemoto-Hori C., Hanada T., Ueda T., Wada A., Watanabe K. Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria. J Biol Chem. 276:2001;21724-21736.
-
(2001)
J Biol Chem
, vol.276
, pp. 21724-21736
-
-
Suzuki, T.1
Terasaki, M.2
Takemoto-Hori, C.3
Hanada, T.4
Ueda, T.5
Wada, A.6
Watanabe, K.7
-
32
-
-
0031813004
-
Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: Toward a human mitochondrial proteome
-
Rabilloud T., Kieffer S., Procaccio V., Louwaggie M., Courchesne P., Patterson S., Martinez P., Garin J., Lunardi J. Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: toward a human mitochondrial proteome. Electrophoresis. 19:1998;1006-1014.
-
(1998)
Electrophoresis
, vol.19
, pp. 1006-1014
-
-
Rabilloud, T.1
Kieffer, S.2
Procaccio, V.3
Louwaggie, M.4
Courchesne, P.5
Patterson, S.6
Martinez, P.7
Garin, J.8
Lunardi, J.9
-
33
-
-
0033769442
-
High-throughput profiling of the mitochondrial proteome using affinity fractionation and automation
-
Lopez M., Kristal B., Chernokalskaya E., Lazarev A., Shestopalov A., Bogdanova A., Robinson M. High-throughput profiling of the mitochondrial proteome using affinity fractionation and automation. Electrophoresis. 21:2000;3427-3440.
-
(2000)
Electrophoresis
, vol.21
, pp. 3427-3440
-
-
Lopez, M.1
Kristal, B.2
Chernokalskaya, E.3
Lazarev, A.4
Shestopalov, A.5
Bogdanova, A.6
Robinson, M.7
-
34
-
-
0000233053
-
Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from a SH-SY5Y neuroblastoma cell line
-
Scheffler N., Miller S., Carroll A., Anderson C., Davis R., Ghosh S., Gibson B. Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from a SH-SY5Y neuroblastoma cell line. Mitochondrion. 1:2001;161-179.
-
(2001)
Mitochondrion
, vol.1
, pp. 161-179
-
-
Scheffler, N.1
Miller, S.2
Carroll, A.3
Anderson, C.4
Davis, R.5
Ghosh, S.6
Gibson, B.7
-
36
-
-
85047683107
-
Proteomic approach to identify novel mitochondrial proteins in Arabidopsis
-
Kruft V., Eubel H., Jansch L., Werhahn W., Braun H. Proteomic approach to identify novel mitochondrial proteins in Arabidopsis. Plant Physiol. 127:2001;1694-1710.
-
(2001)
Plant Physiol
, vol.127
, pp. 1694-1710
-
-
Kruft, V.1
Eubel, H.2
Jansch, L.3
Werhahn, W.4
Braun, H.5
-
37
-
-
0036204065
-
Preparative two-dimensional gel electrophoresis at alkaline pH using narrow range immobilized pH gradients
-
Hoving S., Gerrits B., Voshol H., Muller D., Roberts R., van Oostrum J. Preparative two-dimensional gel electrophoresis at alkaline pH using narrow range immobilized pH gradients. Proteomics. 2:2002;127-134.
-
(2002)
Proteomics
, vol.2
, pp. 127-134
-
-
Hoving, S.1
Gerrits, B.2
Voshol, H.3
Muller, D.4
Roberts, R.5
Van Oostrum, J.6
-
38
-
-
0037563355
-
Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins
-
Görg A., Obermaier C., Boguth G., Csordas A., Diaz J., Madjar J. Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins. Electrophoresis. 18:1997;328-337.
-
(1997)
Electrophoresis
, vol.18
, pp. 328-337
-
-
Görg, A.1
Obermaier, C.2
Boguth, G.3
Csordas, A.4
Diaz, J.5
Madjar, J.6
-
39
-
-
0033847227
-
Improved sensitivity proteomics by post-harvest alkylation and radioactive labeling of proteins
-
Vuong G., Weiss S., Kammer W., Priemer M., Vingron M., Nordheim A., Cahill M. Improved sensitivity proteomics by post-harvest alkylation and radioactive labeling of proteins. Electrophoresis. 21:2000;2594-2605.
-
(2000)
Electrophoresis
, vol.21
, pp. 2594-2605
-
-
Vuong, G.1
Weiss, S.2
Kammer, W.3
Priemer, M.4
Vingron, M.5
Nordheim, A.6
Cahill, M.7
-
40
-
-
0035106351
-
Large scale analysis of the yeast proteome by multidimensional protein identification technology
-
Washburn M., Wolters D., Yates J. Large scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol. 19:2001;242-247.
-
(2001)
Nat Biotechnol
, vol.19
, pp. 242-247
-
-
Washburn, M.1
Wolters, D.2
Yates, J.3
-
41
-
-
0037040827
-
Applied proteomics: Mitochondrial proteins and effect on function
-
Lopez M., Melov S. Applied proteomics: mitochondrial proteins and effect on function. Circ Res. 90:2002;380-389. Useful summary of strategies and accomplishments from a variety of 2DGE/MS studies of the mitochondrial proteome.
-
(2002)
Circ Res
, vol.90
, pp. 380-389
-
-
Lopez, M.1
Melov, S.2
-
42
-
-
0036246088
-
Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry
-
Zhou H., Ranish J., Watts J., Aebersold R. Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry. Nat Biotechnol. 20:2002;512-515.
-
(2002)
Nat Biotechnol
, vol.20
, pp. 512-515
-
-
Zhou, H.1
Ranish, J.2
Watts, J.3
Aebersold, R.4
|