Rainbow's end: The quest for multiplexed fluorescence quantitative analysis in proteomics

Current Opinion in Chemical Biology

Volumn 6, Issue 1, 2002, Pages 63-69

  Patton, Wayne F ^a   Beechem, Joseph M ^a  

^a MOLECULAR PROBES INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; DRUG METABOLIZING ENZYME; FLUORESCENT DYE; GLYCOSYLATED PROTEIN;

ARTICLE; DIFFERENCE GEL ELECTROPHORESIS; ENZYME ASSAY; FEASIBILITY STUDY; FLUORESCENCE ANALYSIS; GEL ELECTROPHORESIS; HUMAN; IMAGE ANALYSIS; LIQUID CHROMATOGRAPHY; MEASUREMENT; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEOMICS; QUANTITATIVE ANALYSIS; SILVER STAINING; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 0036468910     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(01)00284-8     Document Type: Review

References (28)

1. Two-dimensional electrophoresis of membrane proteins using immobilized pH gradients
2. Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology
3. What place polyacrylamide in proteomics?
4. Direct analysis of protein complexes using mass spectrometry
5. Large-scale analysis of the yeast proteome by multidimensional protein identification technology
6. Quantitative proteomic analysis using a MALDI quadrupole time-of-flight mass spectrometer
7. Printing proteins as microarrays for high-throughput function determination
8. Global analysis of protein activities using proteome chips
9. Making blind robots see; the synergy between fluorescent dyes and imaging devices in automated proteomics
10. A thousand points of light; the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics
11. Background-free, high-sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex
12. Ultrasensitive fluorescence protein detection in isoelectric focusing gels using a ruthenium metal chelate stain
13. A comparison of silver stain and SYPRO ruby protein gel stain with respect to protein detection in two-dimensional gels and identification by peptide mass profiling
14. Comparison of three different fluorescent visualization strategies for detection of Escherichia coli ATP synthase subunits after SDS-polyacrylamide gel electrophoresis
15. Difference gel electrophoresis: A single gel method for detecting changes in protein extracts
16. Difference gel electrophoresis
17. Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology
18. Fluorescent dual colour 2D-protein gel electrophoresis for rapid detection of differences in protein pattern with standard image analysis software
19. Changes in the mitochondrial proteome from mouse hearts deficient in creatine kinase
20. Changes in glycolytic network and mitochondrial design in creatine kinase-deficient muscles
21. Automatic registration for images of two-dimensional protein gels
22. Rapid and simple single nanogram detection of glycoproteins in polyacrylamide gels and on electroblots
23. Simultaneous, two color fluorescence detection of total protein profiles and β-glucuronidase activity in polyacrylamide gels and on poly(vinylidene difluoride) membranes
24. Fluorescent Bocillins: Synthesis and application in the detection of penicillin-binding proteins
25. Expression and function of beta-glucuronidase in pancreatic cancer: Potential role in drug targeting
26. Chemical approaches for functionally probing the proteome