Formation of a nucleoprotein complex containing Tn7 and its target DNA regulates transposition initiation

EMBO Journal

Volumn 21, Issue 13, 2002, Pages 3494-3504

  Skelding, Zachary ^a   Sarnovsky, Robert ^b   Craig, Nancy L ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

DNA transposition; DNA protein complex; Enzyme activation; Tn7; Transposase

Indexed keywords

CELL PROTEIN; DNA; NUCLEOPROTEIN; PROTEIN TNSA; PROTEIN TNSB; PROTEIN TNSC; PROTEIN TNSD; TRANSPOSASE; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; DNA PROTEIN COMPLEX; DNA STRAND BREAKAGE; DNA TRANSPOSITION; ENZYME ACTIVITY; GENE TARGETING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; TRANSPOSON;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BACTERIOPHAGE MU; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MACROMOLECULAR SUBSTANCES; MAGNESIUM; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; NUCLEOPROTEINS; PROTEIN INTERACTION MAPPING; PROTEIN STRUCTURE, TERTIARY; RECOMBINATION, GENETIC; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

INSERTION SEQUENCES; TRANSPOSONS;

EID: 0036646490     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdf347     Document Type: Article

References (33)

1. Interaction of the Tn7-encoded transposition protein TnsB with the ends of the transposon
2. Tn7 transposition: Target DNA recognition is mediated by multiple Tn7-encoded proteins in a purified in vitro system
3. MuB protein allosterically activates strand transfer by the transposase of phage Mu
4. A minimal system for Tn7 transposition: The transposon-encoded proteins TnsA and TnsB can execute DNA breakage and joining reactions that generate circularized Tn7 species
5. A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis
6. Tn7
7. Transposition of Mu DNA: Joining of Mu to target DNA can be uncoupled from cleavage at the ends of Mu
8. Nucleoprotein structures initiating DNA replication, transcription and site-specific recombination
9. Purification and characterization of TnsC, a Tn7 transposition protein that binds ATP and DNA
10. Multiple DNA processing reactions underlie Tn7 transposition
11. Higher-order nucleoprotein complexes in transcription: Analogies with site-specific recombination
12. Integrating DNA: Transposases and retroviral integrases
13. Unexpected structural diversity in DNA recombination: The restriction endonuclease connection
14. Target DNA structure plays a critical role in Tn7 transposition
15. Isolation and characterization of Tn7 transposase gain-of-function mutants: A model for transposase activation
16. Switching from cut-and-paste to replicative Tn7 transposition
17. Sequence requirements of Escherichia coli attTn7, a specific site of transposon Tn7 insertion
18. Assembly of the active form of the transposase-Mu DNA complex: A critical control point in Mu transposition
19. A new set of Mu DNA transposition intermediates: Alternate pathways of target capture preceding strand transfer
20. A Tn7-like transposon is present in the glmUS region of the obligately hemoautolithotrophic bacterium Thiobacillus ferrooxidans
21. Tn7 recognizes target structures associated with DNA replication using the DNA binding protein TnsE
22. Tn7: Smarter than we thought
23. The Tn10 Synaptic complex can capture a target DNA only after transposon excision
24. Identification and characterization of a pre-cleavage synaptic complex that is an early intermediate in Tn10 transposition
25. The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products
26. Host proteins can stimulate Tn7 transposition: A novel role for the ribosomal protein L29 and the acyl carrier protein
27. Avoiding self: Two Tn7-encoded proteins mediate target immunity in Tn7 transposition
28. Gain-of-function mutations in TnsC, an ATP-dependent transposition protein which activates the bacterial transposon Tn7
29. Analysis of gain of function mutants of an ATP-dependent regulator of Tn7 transposition
30. Transpososomes: Stable protein-DNA complexes involved in the in vitro transposition of bacteriophage Mu DNA
31. Tn7 transposition: Two transposition pathways directed by five Tn7-encoded genes
32. Three-site synapsis during Mu DNA transposition: A critical intermediate preceding engagement of the active site
33. Conjugating plasmids are preferred targets for Tn7