Analysis of gain-of-function mutants of an ATP-dependent regulator of Tn7 transposition

Journal of Molecular Biology

Volumn 305, Issue 3, 2001, Pages 633-642

  Stellwagen, Anne E ^a   Craig, Nancy L ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

ATP hydrolysis; Control of transposase; Transposase activation; Transposon

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; DNA BINDING PROTEIN; PROTEIN; PROTEIN TNSD; PROTEIN TNSE; TRANSPOSASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA TRANSPOSITION; ENZYME ACTIVATION; GENE FUNCTION; GENE MUTATION; GENE TARGETING; GENETIC CODE; HYDROLYSIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; TRANSPOSON;

EID: 0035910277     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4317     Document Type: Article

References (28)

1. Target immunity of Mu transposition reflects a differential distribution of MuB protein
2. Transposon Tn7 cis-acting sequences in transposition and transposition immunity
3. Tn7 transposition in vitro proceeds through an excised transposon intermediate generated by staggered breaks in DNA
4. Tn7 transposition: Target DNA recognition is mediated by multiple Tn7-encoded proteins in a purified in vitro system
5. A gene regulating the heat shock response in Escherichia coli also affects proteolysis
6. MuB protein allosterically activates strand transfer by the transposase of phage Mu
7. A minimal system for Tn7 transposition: The transposon-encoded proteins TnsA and TnsB can execute DNA breakage and joining reactions that generate circularized Tn7 species
8. A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis
9. Transposon Tn7
10. Purification and characterization of TnsC, a Tn7 transposition protein that binds ATP and DNA
11. Multiple DNA processing reactions underlie Tn7 transposition
12. Recognition of Escherichia coli attTn7 by transposon Tn7: Lack of specific sequence requirements at the point of Tn7 insertion
13. Control of Tn7 transposition
14. Transposition of phage mu DNA
15. Isolation and characterization of Tn7 transposase gain-of-function mutants: A model for transposase activation
16. B protein of bacteriophage Mu is an ATPase that preferentially stimulates intermolecular DNA strand transfer
17. Switching from cutand-paste to replicative Tn7 transposition
18. Sequence requirements of Escherichia coli attTn7, a specific site of transposon Tn7 insertion
19. Transpositional recombination: Mechanistic insights from studies of Mu and other elements
20. The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products
21. Host proteins can stimulate Tn7 transposition: A novel role for the ribosomal protein L29 and the acyl carrier protein
22. G protein mechanisms: Insights from structural analysis
23. Avoiding self: Two Tn7-encoded proteins mediate target immunity in Tn7 transposition
24. Gain-of-function mutations in TnsC, an ATP-dependent transposition protein which activates the bacterial transposon Tn7
25. Mobile DNA elements: Controlling transposition with ATP-dependent molecular switches
26. Stimulation of the Mu DNA strand cleavage and intramolecular strand transfer reactions by the MuB protein is independent of stable binding of the MuB protein to DNA
27. Conjugating plasmids are preferred targets for Tn7
28. An ATP-ADP switch in MuB controls progression of the Mu transposition pathway