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Volumn 272, Issue 5260, 1996, Pages 401-404

Switching from cut-and-paste to replicative Tn7 transposition

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENETICS; DNA RECOMBINATION; DNA REPLICATION; DNA TRANSPOSITION; NONHUMAN; PRIORITY JOURNAL; TRANSPOSON;

EID: 0029984672     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5260.401     Document Type: Article
Times cited : (105)

References (40)
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    • note
    • 2, 14 mM NaCI, 21 mM KCl, 120 μM CHAPS, 4 μM PMSF, 1.2% glycerol, 24 nM TnsA, 3 nM TnsB, 8 nM TnsC, and 6 nM TnsD. Reactions were stopped with 25 mM HDTA.
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    • note
    • We thank P. Eckhoff for help in the preparation of this manuscript; J Boeke and members of the Craig laboratory for critical reading of the manuscript; P. Gary, P. Sharpe, B Sarnovsky, and A. Stellwagen for useful reagents; and M. Biery for developing several critical methods.


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