Role of a conserved acidic cluster in bovine β1,4 galactosyltransferase-1 probed by mutagenesis of a bacterially expressed recombinant enzyme

Glycobiology

Volumn 9, Issue 8, 1999, Pages 815-822

  Zhang, Yingnan ^a   Malinovskii, Vladimir A ^a   Fiedler, Tristan J ^a   Brew, Keith ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

1,4 Galactosyltransferase; Expression in E. coli; Mutagenesis; Reaction mechanism

Indexed keywords

GALACTOSYLTRANSFERASE; MUTANT PROTEIN; RECOMBINANT ENZYME;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CATTLE; CELL INCLUSION; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; ULTRAVIOLET SPECTROSCOPY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; BASE SEQUENCE; CATTLE; CIRCULAR DICHROISM; CONSERVED SEQUENCE; DNA PRIMERS; ESCHERICHIA COLI; GALACTOSYLTRANSFERASES; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; ESCHERICHIA COLI;

EID: 0032766699     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/9.8.815     Document Type: Article

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