Mycolic acid biosynthesis and enzymic characterization of the β-ketoacyl-ACP synthase A-condensing enzyme from Mycobacterium tuberculosis

Biochemical Journal

Volumn 364, Issue 2, 2002, Pages 423-430

  Kremer, Laurent ^a   Dover, Lynn G ^a   Carrère, Séverine ^a   Nampoothiri, K Madhavan ^a   Lesjean, Sarah ^a   Brown, Alistair K ^a   Brennan, Patrick J ^a   Minnikin, David E ^a   Locht, Camille ^a   Besra, Gurdyal S ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Acyl carrier protein; Cerulenin; Fatty acid; Type II fatty acid synthase

Indexed keywords

BACTERIA; BIOSYNTHESIS; CATALYSIS; CONDENSATION; DRUG PRODUCTS; ENZYME INHIBITION; ENZYMES; FATTY ACIDS;

MUTATIONS;

BIOCHEMISTRY;

3 OXOACYL ACYL CARRIER PROTEIN SYNTHASE; ANTIMYCOBACTERIAL AGENT; CERULETIDE; FATTY ACID SYNTHASE; MYCOLIC ACID; RECOMBINANT ENZYME;

ARTICLE; CONTROLLED STUDY; DRUG TARGETING; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME ISOLATION; ENZYME MECHANISM; FATTY ACID SYNTHESIS; IN VITRO STUDY; MYCOBACTERIUM CHELONEI; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ACETYLTRANSFERASES; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; MYCOBACTERIUM TUBERCULOSIS; MYCOLIC ACIDS; SEQUENCE HOMOLOGY, AMINO ACID;

ACTINOBACTERIA (CLASS); MYCOBACTERIUM; MYCOBACTERIUM CHELONAE; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; UNCULTURED ACTINOMYCETE;

EID: 0036606073     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20011628     Document Type: Article

References (31)

1. Molecular action of antimycobacterial agents and mechanisms of drug resistance in Mycobacterium tuberculosis
2. The envelope of mycobacteria
3. Bacterial fatty acid biosynthesis: Targets for antibacterial drug discovery
4. Control mechanisms for fatty acid synthesis in Mycobacterium smegmatis
5. Inhibition of a Mycobacterium tuberculosis β-ketoacyl ACP synthase by isoniazid
6. Identification and substrate specificity of β-ketoacyl (acyl carrier protein) synthase III (mtFabH) from Mycobacterium tuberculosis
7. Genetics of mycolic acid biosynthesis
8. Structure and synthesis of tipids
9. Thiolactomycin and related analogues as novel anti-mycobacterial agents targeting KasA and KasB condensing enzymes in Mycobacteriurn tuberculosis
10. Rapid mycobacterial plasmid analysis by electroduction between Mycobacterium spp. and Escherichia coli
11. Antimycobacterial action of thiolactomycin: An inhibitor of fatty acid and mycolic acid synthesis
12. Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels
13. Biochemical characterization of acyl carrier protein (AcpM) and malonyl-CoA:AcpM transacylase (mtFabD), two major components of Mycobacterium tuberculosis fatty acid synthase II
14. Overproduction of β-ketoacyl-acyl carrier protein synthase I imparts thiolactomycin resistance to Escherichia coli K12
15. Structural, enzymatic, and genetic studies of β-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli
16. The mabA gene from inhA operon of Mycobacteriurn tuberculosis encodes a β-ketoacyl reductase that fails to confer isoniazid resistance
17. InhA, a target of the antituberculous drug isoniazid, is involved in a mycobacterial fatty acid elongation system, FAS-II
18. The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis
19. Inhibition of β-ketoacyl-acyl carrier protein synthases by thiolactomycin and cerulenin. Structure and mechanism
20. Conserved residues in condensing enzyme domains of fatty acid synthases and related sequences
21. Crystal structure of the β-ketoacyl-acyl carrier protein synthase II from E. coli reveals the molecular architecture of condensing enzymes
22. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
23. Isolation and characterization of the β-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12
24. Regulation of fatty acid biosynthesis in Escherichia coli
25. The X-ray crystal structure of β-ketoacyl [acyl carrier protein] synthase I
26. Structure of the complex between the antibiotic cerulenin and its target, β-ketoacyl-acyl carrier protein synthase
27. Purification and biochemical characterization of the Mycobacterium tuberculosis β-ketoacyl-acyl carrier protein synthases KasA and KasB
28. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis
29. New use of BCG for recombinant vaccines