Purification and Biochemical Characterization of the Mycobacterium tuberculosis Β-Ketoacyl-acyl Carrier Protein Synthases KasA and KasB

Journal of Biological Chemistry

Volumn 276, Issue 50, 2001, Pages 47029-47037

  Schaeffer, Merrill L ^a   Agnihotri, Gautam ^a   Volker, Craig ^a   Kallender, Howard ^a   Brennan, Patrick J ^b   Lonsdale, John T ^a  

^a GLAXOSMITHKLINE   (United States)

^b Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; BIOSYNTHESIS; CARBON; CELL MEMBRANES; ENZYMES; ESCHERICHIA COLI; FATTY ACIDS; ORGANIC ACIDS; POLYPEPTIDES;

ANTI-TUBERCULOSIS AGENTS;

BIOCHEMISTRY;

ACYL ACYL CARRIER PROTEIN; ACYL CARRIER PROTEIN; ACYL CARRIER PROTEIN SYNTHASE KASA; ACYL CARRIER PROTEIN SYNTHASE KASB; ANTIMYCOBACTERIAL AGENT; CERULENIN; ENZYME INHIBITOR; FATTY ACID DERIVATIVE; FATTY ACID SYNTHASE 1; MALONYL ACYL CARRIER PROTEIN; MYCOLIC ACID; PROTEIN PRECURSOR; PROTEINASE; THIOLACTOMYCIN; TUBERCULOSTATIC AGENT; UNCLASSIFIED DRUG; 3 OXOACYL (ACYL CARRIER PROTEIN) SYNTHASE; 3 OXOACYL ACYL CARRIER PROTEIN SYNTHASE; ANTIBIOTIC AGENT; ANTIFUNGAL AGENT; CARBON; FATTY ACID; FATTY ACID SYNTHASE II, BACTERIAL; KASB PROTEIN; THIOPHENE DERIVATIVE;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL CELL WALL; BINDING AFFINITY; BIOCHEMISTRY; CONTROLLED STUDY; DISSOCIATION; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; INHIBITION KINETICS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; STRUCTURE ANALYSIS; BIOSYNTHESIS; CHEMICAL MODEL; CHEMISTRY; DOSE RESPONSE; ENZYME SPECIFICITY; ENZYMOLOGY; GENE DELETION; GENETICS; IC 50; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PHYLOGENY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; TIME;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

3-OXOACYL-(ACYL-CARRIER-PROTEIN) SYNTHASE; ANTIBIOTICS; ANTIBIOTICS, ANTIFUNGAL; CARBON; CERULENIN; CHROMATOGRAPHY, AFFINITY; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; ESCHERICHIA COLI; FATTY ACIDS; GENE DELETION; INHIBITORY CONCENTRATION 50; KINETICS; MODELS, CHEMICAL; MYCOBACTERIUM TUBERCULOSIS; MYCOLIC ACIDS; PHYLOGENY; PROTEIN BINDING; SUBSTRATE SPECIFICITY; SUPPORT, U.S. GOV'T, P.H.S.; THIOPHENES; TIME FACTORS;

EID: 0035861550     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M108903200     Document Type: Article

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