Alteration of pore properties of Escherichia coli OmpF induced by mutation of key residues in anti-loop 3 region

Biochemical Journal

Volumn 363, Issue 3, 2002, Pages 521-528

  Bredin, Jérôme ^a   Saint, Nathalie ^a   Malléa, Monique ^a   Dé, Emmanuelle ^a   Molle, Gérard ^a   Pagès, Jean Marie ^a   Simonet, Valérie ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Diffusion; Mutant; OmpF porin; Planar bilayer; Voltage gating

Indexed keywords

BIOASSAY; DATA REDUCTION; DIFFUSION; ESCHERICHIA COLI; LIPIDS; MUTAGENESIS; THERMODYNAMIC STABILITY;

MEMBRANE POTENTIAL;

BIOCHEMICAL ENGINEERING;

ARGININE; CEFEPIME; CEPHALOSPORIN DERIVATIVE; ION CHANNEL; LIPOSOME; LYSINE; MUTANT PROTEIN; OUTER MEMBRANE PROTEIN F; PORIN; SUGAR; UNCLASSIFIED DRUG;

ARTICLE; CELL MEMBRANE CONDUCTANCE; CELL MEMBRANE POTENTIAL; CELL MEMBRANE TRANSPORT; CELL SWELLING; CHANNEL GATING; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DIFFUSION; DRUG TRANSPORT; ELECTRIC POTENTIAL; ESCHERICHIA COLI; IN VITRO STUDY; IN VIVO STUDY; LIPID BILAYER; MOLECULAR MODEL; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; SURFACE CHARGE; THERMOSTABILITY;

ARGININE; CEPHALOSPORINS; DIFFUSION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; ION CHANNEL GATING; LIPID BILAYERS; LYSINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PORINS; STRUCTURE-ACTIVITY RELATIONSHIP;

ESCHERICHIA COLI;

EID: 0036566425     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3630521     Document Type: Article

References (46)

1. Prevention of drug access to bacterial targets: Permeability barriers and active efflux
2. Outer membrane
3. Crystal structures explain functional properties of two E. coli porins
4. Electrostatic properties of two porin channels from Escherichia coli
5. Voltage gating of Escherichia coli porin channels: Role of the constriction loop
6. Molecular basis for the polyamine-ompF porin interactions: Inhibitor and mutant studies
7. Voltage-gating of Escherichia coli porin: A cystine-scanning mutagenesis study of loop 3
8. Structural and functional alterations of a colicin-resistant mutant of OmpF porin from Escherichia coli
9. Substitutions in the eyelet region disrupt celepime diffusion through the Escherichia coli OmpF channel
10. Mutations that alter the pore function of the OmpF porin of Escherichia coli K12
11. Structural and functional characterization of OmpF porin mutants selected for larger pore size. II. Functional characterization
12. Structural and functional characterization of OmpF porin mutants selected for larger pore size. I. Crystallographic analysis
13. Voltage sensing in the PhoE and OmpF outer membrane porins of Escherichia coli: Role of charged residues
14. Coupling site-directed mutagenesis with high-level expression: Large scale production of mutant porins from E. coli
15. Normal precursors of periplasmic proteins accumulated in the cytoplasm are not exported post-translationally in Escherichia coli
16. Comparative protein modeling by satisfaction of spatial restraints
17. SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modeling
18. In vivo modification of porin activity conferring antibiotic resistance to Enterobacter aerogenes
19. Porin channels in Escherichia coli: Studies with liposomes reconstituted from purified proteins
20. Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels
21. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties
22. Involvement of exposed polypeptide loops in trimeric stability and membrane insertion of Escherichia coli OmpF porin
23. Stability of trimeric OmpF porin: The contributions of the latching loop L2
24. Replacement of the sole histidinyl residue in OmpF porin from E. coli by threonine (H21T) does not affect channel structure and function
25. Fast and slow kinetics of porin channels from Escherichia coli reconstituted into giant liposomes and studied by patch-clamp
26. Function and modulation of bacterial porins: Insights from electrophysiology
27. Cefepime: Overview of activity in vitro and in vivo
28. Outer membrane permeability and beta-lactamase stability of dipolar ionic cephalosporins containing methoxyimino substituents
29. Porin mutants with new channel properties
30. Identification of positively charged residues of FomA porin ol Fusobacterium nucleatum which are important for pore function
31. Pore formation by pho-controlled outer-membrane proteins of various Enterobacteriaceae in lipid bilayers
32. X-ray crystallographic and mass spectrometric structure determination and functional characterization of succinylated porin from Rhodobacter capsulatus: Implications for ion selectivity and single-channel conductance
33. Ion selectivity reversal and induction of voltage-gating by site-directed mutations in the Paracoccus denitrificans porin
34. Matrix protein from Escherichia coli outer membranes forms voltage-controlled channels in lipid bilayers
35. The voltage-dependent activity of Escherichia coli porins in different planar bilayer reconstitutions
36. Voltage and pH-induced channel closure of porin OmpF visualized by atomic force microscopy
37. OmpC mutants which allow growth on maltodextrins show increased channel size and greater voltage sensitivity
38. Voltage-sensitive ion channel of Escherichia coli
39. A single amino acid substitution alters conductance and gating of OmpC porin of Escherichia coli
40. E. coli PhoE porin has an opposite voltage-dependence to the homologous OmpF
41. A grand canonical Monte Carlo-Brownian dynamics algorithm for simulating ion channels
42. Porin channels in intact cells of Escherichia coli are not affected by Donnan potentials across the outer membrane
43. Role of charged residues at the OmpF porin channel constriction probed by mutagenesis and simulation
44. Diffusion of beta-lactam antibiotics through the porin channels of Escherichia coli K-12
45. Multiple facets of bacterial porins