X-ray crystallographic and mass spectrometric structure determination and functional characterization of succinylated porin from Rhodobacter capsulatus: Implications for ion selectivity and single-channel conductance

Protein Science

Volumn 5, Issue 8, 1996, Pages 1477-1489

  Przybylski, Michael ^a   Glocker, Michael O ^a   Nestel, Uwe ^a   Schnaible, Volker ^a   Blüggel, Martin ^a   Diederichs, Kay ^a   Weckesser, Jurgen ^b   Schad, Michael ^c   Schmid, Angela ^c   Welte, Wolfram ^a   Benz, Roland ^c  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

cation selectivity; charge at pore mouth; crystallography; mass spectrometry; porin ion channels; single channel conductance; succinylated porin; succinylation sites

Indexed keywords

BACTERIAL PROTEIN; ION CHANNEL; LYSINE; PORIN; SUCCINIC ANHYDRIDE;

ARTICLE; CHEMICAL MODIFICATION; CONTROLLED STUDY; ION CONDUCTANCE; LIPID BILAYER; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; RHODOBACTER CAPSULATUS; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); RHODOBACTER CAPSULATUS; RHODOPSEUDOMONAS;

EID: 9344223315     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050804     Document Type: Article

References (45)

1. Bauer K, Struyve M, Bosch D, Benz R, Tommassen J. 1989. One single lysine-residue is responsible for the special interaction between polyphosphate and the outer membrane porin PhoE of Escherichia coli. J Biol Chem 264:16393-16398.
2. Benz R. 1988. Structure and function of porins from Gram-negative bacteria. Annu Rev Microbiol 42:359-393.
3. Benz R, Bauer K. 1988. Permeation of hydrophilic molecules through the outer membrane of Gram-negative bacteria. Eur J Biochem 176:1-19.
4. Benz R, Hardie KR, Hughes C. 1994. Pore-formation in artificial membranes by the secreted hemolysins of Proteus vulgaris and Morganella morganii. Eur J Biochem 220:339-347.
5. Benz R, Janko K, Lauger P. 1979. Ionic selectivity of pores formed by the matrix protein porin of Escherichia coli. Biochim Biophys Acta 551: 238-247.
6. Benz R, Schmid A, Hancock REW. 1985. Ion selectivity of Gram-negative bacterial porins. J Bacteriol 162:722-727.
7. Benz R, Schmid A, Wagner W, Goebel W. 1989. Pore formation by the Escherichia coli hemolysin: Evidence for an association-dissociation equilibrium of the pore-forming aggregates. Infect Immun 57:887-895.
8. Benz R, Tokunaga H, Nakae T. 1984. Properties of chemically modified porin from Escherichia coli in lipid bilayer membranes. Biochim Biophys Acta 769:348-356.
9. Benz R, Woitzik D, Flammann HT, Weckesser J. 1987. Pore forming activity of the major outer membrane protein of Rhodobacter capsulatus in lipid bilayer membranes. Arch Microbiol 14:226-230.
10. Brunen M, Engelhardt H, Schmid A, Benz R. 1991. The major outer membrane protein of Acidovorax delafieldii is an anion-selective porin. J Bacteriol 173:4182-4187.
11. Brünger AT. 1988. Cristallographic refinement by simulated annealing: Application to a 2.8 Å resolution structure of aspartate aminotransferase. J Mol Biol 203:80.
12. Brudie;nger AT. 1993. Assessment of phase accuracy by cross validation: The free R value. Methods and applications. Acta Crystallogr D 49:24-36.
13. Cowan SW, Schirmer T, Rummel G, Steiert M, Ghosh R, Pauptit RA, Jansonius JN, Rosenbusch JP. 1992. Crystal structures explain functional properties of two E. coli porins. Nature 358:727-733.
14. Dani JA. 1986. Ion-channel entrances influence permeation. Biophys J 49:607-618.
15. Fontana A, Gross E. 1986. Fragmentation of polypeptides by chemical methods. In: Darbre A, ed. Practical protein chemistry - A handbook. Chichester: John Wiley & Sons. pp 83-88.
16. Glocker MO, Borchers C, Fiedler W, Suckau D, Przybylski M. 1994. Molecular characterization of surface topology in protein tertiary structures by amino-acylation and mass spectrometric peptide mapping. Bioconj Chem 5:583-591.
17. Hille B. 1991. Ionic channels of excitable membranes. Sunderland, Massachusetts: Sinauer Assoc. Inc.
18. Jones TA, Zou JY, Cowan SW, Kjeldgaard M. 1991. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr A 47:110-119.
19. Jordan PC. 1987. How pore mouth charge distributions alter the permeability of transmembrane ionic channels. Biophys J 51:297-311.
20. Kabsch W. 1988. Automatic indexing of rotation diffraction patterns. J Appl Crystallogr 21:67-71.
21. Karas M, Bahr U, Gießmann U. 1991. Matrix-assisted laser desorption ionization mass spectrometry. Mass Spectrom Rev 10:335-358.
22. Kreusch A, Neubuser A, Schiltz E, Weckesser J, Schulz GE. 1994. Structure of the membrane channel porin from Rhodopseudomonas blastica at 2.0 Å resolution. Protein Sci 3:58-63.
23. Kreusch A, Weiss MS, Welte W, Weckesser J, Schulz GE. 1991. Crystals of an integral membrane protein diffracting to 1 8 Å resolution. J Mol Biol 217:9-10.
24. Lauger P. 1973. Ion transport through pores: A rate-theory analysis. Biochim Biophys Acta 311:423-441.
25. + channel. Biochemistry 28:8092-8099.
26. Menestrina R, Antolini R. 1981. Ion transport through hemocyanin channels in oxidised cholesterol membranes. Biochim Biophys Acta 643:616-625.
27. Nelson AP, McQuarrie DA. 1975. The effect of discrete charges on the electrical properties of a membrane. J Theor Biol 55:13-27.
28. Nestel U, Wacker T, Woitzik D, Weckesser J, Kreutz W, Welte W. 1989. Crystallization and preliminary X-ray analysis of porin from Rhodobacter capsulatus. FEBS Lett 242:405-408.
29. Nielsen PF, Schneider K, Suckau D, Landis B, Przybylski M. 1990. Applications of selective chemical reactions in combination with 252-californium plasma desorption mass spectrometry in protein structure analysis. In: Hilf E, ed. Mass spectrometry of large non-volatile molecules, vol 3. London: World Scientif. pp 194-207.
30. Przybylski M. 1995. Mass spectrometric approaches to the characterization of tertiary and supramolecular structures of biomacromolecules. Adv Mass Spectrom 13:257-285.
31. Przybylski M, Borchers C, Suckau D, Mák M, Jetschke M. 1993. Selective chemical modification and mass spectrometric peptide mapping: A new approach for the molecular characterization of surface topology and micro-environment in protein tertiary structures. In: Schneider CH, Eberle AN, eds. Peptides 1992. Amsterdam: Escom Science Publ. pp 915-916.
32. Przybylski M, Glocker MO. 1996. Electrospray mass spectrometry of non-covalent complexes of biomacromolecules - New analytical perspectives for supramolecular chemistry and molecular recognition processes. Angew Chem Int Ed Engl. 35:806-826.
33. Rietschel ET, Brade L, Schade U, Seydel U, Zahringer U, Kusumoto S, Brade H. 1988. Bacterial endotoxins: Properties and structure of biologically active domains. In: Schrinner E, Richmond MH, Seinbert G, Schwarz U, eds. Surface structures of microorganisms and their interactions with the mammalian host. pp 1-41.
34. Ropele M, Menestrina G. 1989. Electrical properties and molecular architecture of the channel formed by Escherichia coli hemolysin in planar lipid membranes. Biochim Biophys Acta 985:9-18.
35. Sakmann B, Trube G. 1984. Voltage-dependent inactivation of inward-rectifying single-channel currents in the guinea-pig heart cell membrane. J Physiol 347:659-683.
36. Schiltz E, Kreusch A, Nestel U, Schulz GE. 1991. Primary structure of porin from Rhodobacter capsulatus. Eur J Biochem 199:587-594.
37. Schirmer T, Keller TA, Wang YF, Rosenbusch JP. 1995. Structural basis for sugar translocation through maltoporin channels at 3.1 Å resolution. Science 267:512-514.
38. Schmid A, Benz R, Schink B. 1991. Identification of two porins in Pelobacter venetianus fermenting high molecular mass polyethylene glycols. J Bacteriol 173:4909-4913.
39. Smith RD, Loo JA, Orgorzalek Loo RR, Busman M, Udseth HR. 1991. Principles and practice of electrospray ionization-mass spectrometry for large polypeptides and proteins. Mass Spectrom Rev 10:359-401.
40. Suckau D, Mák M, Przybylski M. 1992. Protein surface topology probing by selective chemical modification and mass spectrometric peptide mapping. Proc Natl Acad Sci USA 89:5630-5634.
41. Tokunaga H, Tokunaga M, Nakae T. 1981. Permeability properties of chemically modified porin trimers from Escherichia coli B. J Biol Chem 256: 8024-8029.
42. Weiss MS, Abele U, Weckesser J, Weite W, Schiltz E, Schulz GE. 1991a. Molecular architecture and electrostatic properties of a bacterial porin. Science 254:1627-1630.
43. Weiss MS, Kreusch A, Schiltz E, Nestel U, Welte W, Weckesser J, Schulz GE. 1991b. The structure of porin from Rhodobacter capsulatus at 1.8 Å resolution. FEBS Lett 280:379-382.
44. Weiss MS, Schulz GE. 1992. Structure of porin refined at 1.8 Å resolution. J Mol Biol 227:493-509.
45. Welte W, Nestel U, Wacker T, Diederichs K. 1995. Structure and function of the porin channel. Kidney International 48:930-940.