Coupling site-directed mutagenesis with high-level expression: Large scale production of mutant porins from E. coli

FEMS Microbiology Letters

Volumn 163, Issue 1, 1998, Pages 65-72

  Prilipov, Alexej ^a   Phale, Prashant S ^a   Van Gelder, Patrick ^a   Rosenbusch, Jurg P ^a   Koebnik, Ralf ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

E. coli porin; Gene inactivation; High level expression; Outer membrane protein; Site directed mutagenesis

Indexed keywords

OUTER MEMBRANE PROTEIN; PORIN;

ARTICLE; BACTERIAL MUTATION; ESCHERICHIA COLI; GENE ACTIVATION; GENE EXPRESSION REGULATION; MOLECULAR CLONING; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS;

ADENOSINE TRIPHOSPHATASES; BACTERIAL PROTEINS; BASE SEQUENCE; DNA REPAIR; DNA-BINDING PROTEINS; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; MUTS DNA MISMATCH-BINDING PROTEIN; PORINS; RECEPTORS, VIRUS; RECOMBINANT FUSION PROTEINS; VIRAL PROTEINS;

EID: 0032102987     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00151-7     Document Type: Article

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