Porin mutants with new channel properties

Protein Science

Volumn 7, Issue 7, 1998, Pages 1603-1611

  Schmid, Benedikt ^a   Maveyraud, Laurent ^a   Krömer, Markus ^a   Schulz, Georg E ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Lipid bilayer experiments; Membrane channel; Pore eyelet mutants; Rhodopseudomonas blastica; Voltage gating; X ray structure analysis

Indexed keywords

AMINO ACID SEQUENCE; ARTICLE; CHANNEL GATING; DIFFUSION; ESCHERICHIA COLI; GENE MUTATION; LIPID BILAYER; PRIORITY JOURNAL; RHODOPSEUDOMONAS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; X RAY ANALYSIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; RHODOBACTER BLASTICUS; RHODOPSEUDOMONAS;

EID: 0031853989     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070714     Document Type: Article

References (38)

1. Bauer K, Struyvé M, Bosch D, Benz R, Tommassen J. 1989. One single lysine residue is responsible for the special interaction between polyphosphate and the outer membrane porin PhoE of Escherichia coli. J Biol Chem 264:16393-16398.
2. Benson SA, Occi JLL, Sampson BA. 1988. Mutations that alter the pore function of the OmpF porin of Escherichia coli K12. J Mol Biol 203:961-970.
3. Benz R. 1994. Permeation of hydrophilic solutes through mitochondrial outer membranes: Review on mitochondrial porins. Biochim Biophys Acta 1197: 167-196.
4. Benz R, Bauer K. 1988. Permeation of hydrophilic molecules through the outer membrane of Gram-negative bacteria. Eur J Biochem 176:1-19.
5. Bishop ND, Lea EJA, Mobasheri H, Spiro S. 1996. Altered voltage sensitivity of mutant OmpC porin channels. FEBS Lett 379:295-298.
6. Braha O, Walker B, Cheley S, Kasianowicz JJ, Song L, Gouaux JE, Bayley H. 1997. Designed protein pores as components for biosensors. Chem Biol 4:497-505.
7. Brünger AT. 1993. X-PLOR version 3.1: A system for X-ray crystallography and NMR. New Haven. CT: Yale University Press.
8. Butz S, Benz R, Wacker T, Welte W, Lustig A, Plapp R, Weckesser J. 1993. Biochemical characterization and crystallization of porin from Rhodopseudomonas blastica. Arch Microbiol 159:301-307.
9. De Cock H, van Blokland S, Tommassen J. 1996. In vitro insertion and assembly of outer membrane protein PhoE of Escherichia coli K-12 into the outer membrane. Role of Triton-X-100. J Biol Chem 271:12885-12890.
10. Gill SC, von Hippel PH. 1989. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 182:319-326.
11. Gokce I, Bainbridge G, Lakey JH. 1997. Stabilising and destabilising modifications of cysteines in the E. coli outer membrane porin protein OmpC. FEBS Lett 411:201-205.
12. Jeanteur D, Schirmer T, Fourel D, Simonet V, Rummel G, Widmer C, Rosenbusch JP, Pattus F, Pagès J-M. 1994. Structural and functional alterations of a colicin-resistant mutant of OmpF porin from Escherichia coli. Proc Natl Acad Sci USA 91:10675-10679.
13. Kabsch W. 1988. Evaluation of single-crystal X-ray diffraction data from a position-sensitive detector. J Appl Crystallogr 21:916-924.
14. Kraulis PJ. 1991. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures. J Appl Crystallogr 24:946-950.
15. Kreusch A, Neubüser A, Schiltz E, Weckesser J, Schulz GE. 1994. Structure of the membrane channel porin from Rhodopseudomonas blastica at 2.0 Å resolution. Protein Sci 3:58-63.
16. Kreusch A, Schulz GE. 1994. Refined structure of the porin from Rhodopseudomonas blastica. Comparison with the porin from Rhodobacter capsulatus. J Mol Biol 243:891-905.
17. Lamzin VS, Wilson KS. 1993. Automated refinement of protein models. Acta Crystallogr D49:129-147.
18. Le Dain AC, Häse CC, Tommassen J, Martinac B. 1996. Porins of Escherichia coli: Unidirectional gating by pressure. EMBO J 15:3524-3528.
19. Leslie AGW. 1987. Profile fitting. In: Helliwell JR, Machin PA, Papiz MZ, eds. Computational aspects of protein crystal data analysis. Proceedings of the Daresbury study weekend. Warrington, UK: Daresbury Laboratory. pp 39-50.
20. Lou KL, Saint N, Prilipov A, Rummel G, Benson SA, Rosenbusch JP, Schirmer T. 1996. Structural and functional characterization of OmpF porin mutants selected for larger pore size. I. Crystallographic analysis. J Biol Chem 271:20669-20675.
21. Misra R, Benson SA. 1988. Isolation and characterization of OmpC porin mutants with altered pore properties. J Bacteriol 170:528-533.
22. Murshudov GN, Vagin AA, Dodson EJ. 1997. Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D53:240-255.
23. Nicholls A, Sharp KA, Honig B. 1991. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins Struct Funct Genet 11:281-296.
24. Nikaido H. 1994. Porins and specific diffusion channels in bacterial outer membranes. J Biol Chem 269:3905-3908.
25. Ponder JW, Richards FM. 1987. Tertiary templates for proteins. Use of packing criteria in the enumeration of allowed sequences for different structural classes. J Mol Biol 193:775-791.
26. Przybylski M, Glocker MO, Nestel U, Schnaible V, Blüggel M, Diederichs K, Weckesser J, Schad M, Schmid A, Welte W, Benz R. 1996. X-ray crystallographic and mass spectrometric structure determination and functional characterization of succinylated porin from Rhodobacter capsulatus: Implications for ion selectivity and single-channel conductance. Protein Sci 5:1477-1489.
27. Rocque WJ, McGroarty EJ. 1990. Structure and function of an OmpC deletion mutant porin from Escherichia coli K-12. Biochemistry 29:5344-5351.
28. Saint N, Lou KL, Widmer C, Luckey M, Schirmer T, Rosenbusch JP. 1996a. Structural and functional characterization of OmpF porin mutants selected for larger pore size. II. Functional characterization. J Biol Chem 271:20676-20680.
29. Saint N, Prilipov A, Hardmeyer A, Lou KL, Schirmer T, Rosenbusch JP. 1996b. Replacement of the sole histidinyl residue in OmpF porin from E. coli by threonine (H21T) does not affect channel structure and function. Biochem Biophys Res Commun 223:118-122.
30. Schmid B, Krömer M, Schulz GE. 1996. Expression of porin from Rhodopseudomonas blastica in Escherichia coli inclusion bodies and folding into exact native structure. FEBS Lett 381:111-114.
31. Schulz GE. 1993. Bacterial porins: Structure and function. Curr Opin Cell Biol 5:701-707.
32. Schulz GE. 1996. Porins: General to specific, native to engineered passive pores. Curr Opin Struct Biol 6:485-490.
33. Soares CM, Björkstén J, Tapia O. 1995. L3 loop-mediated mechanisms of pore closing in porin: A molecular dynamics perturbation approach. Protein Eng 8:5-12.
34. Srikumar R, Dahan D, Arhin FF, Tawa P, Diederichs K, Coulton JW. 1997. Porins of Haemophilus influenzae type b mutated in loop 3 and in loop 4. J Biol Chem 272:13614-13621.
35. Taylor JW, Ott J, Eckstein F. 1985. The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA. Nucleic Acids Res 13:8765-8785.
36. Van Gelder P, Saint N, van Boxtel R, Rosenbusch JP, Tommassen J. 1997. Pore functioning of outer membrane protein PhoE of Escherichia coli: Mutagenesis of the constriction loop L3. Protein Eng 10:699-706.
37. Weiss MS, Schulz GE. 1992. Structure of porin refined at 1.8 Å resolution. J Mol Biol 227:493-509.
38. Weiss MS, Wacker T, Weckesser J, Welte W, Schulz GE. 1990. The three-dimensional structure of porin from Rhodobacter capsulatus at 3 Å resolution. FEBS Lett 267:268-272.