Visualisation of the actin cytoskeleton by cryo-electron microscopy

Journal of Cell Science

Volumn 115, Issue 9, 2002, Pages 1877-1882

  Resch, Guenter P ^a   Goldie, Kenneth N ^a   Krebs, Angelika ^a   Hoenger, Andreas ^a   Small, J Victor ^a  

^a Institute of Molecular Biology Salzburg   (Austria)

Author keywords

Actin; Cryo electron microscopy; Cytoskeleton; Lamellipodium; Motility

Indexed keywords

ACTIN;

ANIMAL CELL; ARTICLE; CELL MOTILITY; CELL ULTRASTRUCTURE; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; CYTOSKELETON; DEHYDRATION; LAMELLIPODIUM; MOUSE; NONHUMAN; PRIORITY JOURNAL; ANIMAL; ARTIFICIAL MEMBRANE; CELL CULTURE; CELL MOTION; EUKARYOTIC CELL; INSTRUMENTATION; METABOLISM; METHODOLOGY; MICROFILAMENT; PHYSIOLOGY; PSEUDOPODIUM; ULTRASTRUCTURE;

ANIMAL; CELL MOVEMENT; CRYOELECTRON MICROSCOPY; EUKARYOTIC CELLS; MEMBRANES, ARTIFICIAL; MICE; MICROFILAMENTS; PSEUDOPODIA; TUMOR CELLS, CULTURED; ANIMALS;

EID: 0036558286     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (42)

1. The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella
2. Focusing-in on microtubules
3. Adding the third dimension to virus life cycles: Three-dimensional reconstruction of icosahedral viruses from cryo-electron micrographs
4. Actin dynamics in living mammalian cells
5. Structural analysis of the microtubule-kinesin complex by cryo-electron microscopy
6. Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins
7. Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy
8. Filamin A, the Arp2/3 complex, and the morphology and function of cortical actin filaments in human melanoma cells
9. A model of protein synthesis based on cryo-electron microscopy of the E. coli ribosome
10. Energy filtered electron tomography of ice-embedded actin and vesicles
11. Scar/WAVE is localised at the tips of protruding lamellipodia in living cells
12. The architecture of actin filaments and the ultrastructural location of actin-binding protein in the periphery of lung macrophages
13. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy
14. Filament organization revealed in platinum replicas of freeze-dried cytoskeletons
15. Regulation of actin polymerization by Arp2/3 complex and WASp/Scar proteins
16. Direct labeling of components in protein complexes by immuno-electron microscopy
17. Formation of cell-to-substrate contacts during fibroblast motility: An interference-reflexion study
18. Small angle X-ray scattering and electron cryomicroscopy study of actin filaments: Role of the bound nucleotide in the structure of F-actin
19. Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex
20. Actin filament destruction by osmium tetroxide
21. Electron microscopy of cells: A new beginning for a new century
22. Nicotinic acetylcholine receptor at 4.6 A resolution: Transverse tunnels in the channel wall
23. The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments
24. High voltage cryomicroscopy of human blood platelets
25. Mechanism of actin-based motility
26. Pure F-actin networks are distorted and branched by steps in the critical point drying method
27. Contact formation during fibroblast locomotion: Involvement of membrane ruffles and microtubules
28. Leading edge movement and ultrastructure in mouse macrophages
29. VASP dynamics during lamellipodia protrusion
30. Structure of Lumbricus terrestris hemoglobin at 30 Å resolution determined using angular reconstitution
31. Organization of actin in the leading edge of cultured cells: Influence of osmium tetroxide and dehydration on the ultrastructure of actin meshworks
32. Factors affecting the integrity of actin meshworks in cultured cells
33. The actin cytoskeleton
34. Whole-mount electron microscopy of the cytoskeleton: Negative staining methods
35. Organization of actin meshworks in cultured cells: The leading edge
36. Visualising the Actin Cytoskeleton
37. The lamellipodium: Where motility begins
38. Correlative light and electron microscopy of the cytoskeleton of cultured cells
39. Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament arrays in lamellipodia
40. Improved procedure for electron microscopic visualization of the cytoskeleton of cultured cells
41. Structure of Arp2/3 complex in its activated state and in actin filament branch junctions
42. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly