Noncovalent interaction energies in covalent complexes: Tem-1 β-lactamase and β-lactams

Proteins: Structure, Function and Genetics

Volumn 47, Issue 1, 2002, Pages 86-96

  Wang, Xiaojun ^a   Minasov, George ^a   Shoichet, Brian K ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

Author keywords

lactamase; Antibiotic resistance; Protein stability; TEM 1

Indexed keywords

ASPARAGINE; BETA LACTAM ANTIBIOTIC; BETA LACTAMASE; BETA LACTAMASE INHIBITOR; BETA LACTAMASE TEM1; IMIPENEM; LATAMOXEF; SERINE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; COVALENT BOND; ENERGY TRANSFER; ENTHALPY; ENZYME BINDING; EQUILIBRIUM CONSTANT; INHIBITION KINETICS; MOLECULAR INTERACTION; MOLECULAR STABILITY; NONHUMAN; PHASE TRANSITION; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SUBSTITUTION; ANTI-BACTERIAL AGENTS; BETA-LACTAMASES; BETA-LACTAMS; CRYSTALLOGRAPHY, X-RAY; ENERGY METABOLISM; ENZYME INHIBITORS; ENZYME STABILITY; IMIPENEM; MACROMOLECULAR SUBSTANCES; MODELS, CHEMICAL; MODELS, MOLECULAR; MOXALACTAM; PROTEIN CONFORMATION; PROTEIN DENATURATION; TEMPERATURE;

BACTERIA (MICROORGANISMS);

EID: 0036533471     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.10058     Document Type: Article

References (37)

1. β-Lactamases in laboratory and clinical resistance
2. Quantitive analysis of the stabilization by s ubstrate of Staphylococcus aureus PC1 β-lactamase
3. Molecular structure of the acyl-enzyme intermediate in β-lactam hydrolysis at 1.7 Å resolution
4. Inhibition of β-lactamase by clavulanate. Trapped intermediates in cryocrystallographic studies
5. Structure of a phosphonate-inhibited β-lactamase. An analog of the tetrahedral transition state/intermediate of β-lactam hydrolysis
6. Crystal structure of Escherichia coli TEM-1 β-lactamase at 1.8 Å resolution
7. Structural basis for clinical longevity of carbapenem antibiotics in the face of challenge by the common class A β-lactamases from the antibiotic-resistant bacteria
8. Characterization of covalently bound enzyme inhibitors as transition-state analogs by protein stability measurements: Phosphonate monoester inhibitors of a β-lactamase
9. Energetic origins of specificity of ligand binding in an interior nonpolar cavity of T4 lysozyme
10. Functional analyses of AmpC β-lactamase through differential stability
11. Interaction energies between β-lactam antibiotics and E. coli penicillin-binding protein 5 by reversible thermal denaturation
12. β-Lactamases of increasing clinical importance
13. Molecular evolution of bacterial β-lactam resistance
14. Kinship and diversification of bacterial penicillin-binding proteins and β-lactamases
15. Crystal structures of substrate and inhibitor complexes with AmpC-lactamase: Possible implications for substrate-assisted catalysis
16. Inhibition of AmpC beta- lactamase through a destabilizing interaction in the active site
17. Catalytic mechanism of active-site serine beta-lactamases: Role of the conserved hydroxy group of the Lys-Thr(Ser)-Gly triad
18. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
19. Protein stability curves
20. Processing of X-ray diffraction data collected in oscillation mode
21. Crystallography and NMR system: A new software suite for macromolecular structure determination
22. IsoStar: A library of information about nonbonded interactions
23. Accurate bond and angle parameters for X-ray protein structure refinement
24. Structure-activity studies of the inhibition of serine β-lactamases by phosphonate monoesters
25. Energetic, structural, and antimicrobial analyses of β-lactam side chain recognition by β-lactamases
26. Role of the conserved amino acids of the 'SDN' loop (Ser130, Asp131 and Asn132) in a class A β-lactamase studied by site-directed mutagenesis
27. PROCHECK: A program to check the stereochemical quality of protein structures
28. Evidence for an oxyanion hole in serine beta-lactamases and DD-peptidases
29. Three-dimensional structure of AmpC β-lactamase from Escherichia coli bound to a transition-state analogue: Possible implications for the oxyanion hypothesis and for inhibitor design
30. Substitution of Asp for Asn at position 132 in the active site of TEM β-lactamase. Activity toward different substrates and effects of neighboring residues
31. β-Lactamase-mediated resistance and opportunities for its control
32. Analysis of a carbapenem-hydrolyzing class A β-lactamase from Enterobacter cloacae and of its LysR-type regulatory protein
33. Characterization of IMI-1 β-lactamase, a class A carbapenem-hydrolyzing enzyme from Enterobacter cloacae
34. Biochemical properties of a carbapenem-hydrolyzing β-lactamase from Enterobacter cloacae and cloning of the gene into Escherichia coli
35. X-ray analysis of the NMC-A β-lactamase at 1.64-Å resolution, a class A carbapenemase with broad substrate specificity