Flow cytometry used for the analysis of calcium signaling induced by antigen-specific T-cell activation

Cytometry

Volumn 47, Issue 4, 2002, Pages 207-216

  Réthi, Bence ^a,c   Detre, Cynthia ^a   Gogolák, Péter ^a   Kolonics, Attila ^b   Magócsi, Mária ^b   Rajnavölgyi, Éva ^a  

^a EÖTVÖS LORÁND UNIVERSITY   (Hungary)

^b National Institute of Haematology and Immunology Budapest   (Hungary)

^c UNIVERSITY OF DEBRECEN   (Hungary)

Author keywords

Antigen presenting cell; Calcium signal; Costimulation; T cell activation

Indexed keywords

B7 ANTIGEN; CALCIUM; CD28 ANTIGEN; CD86 ANTIGEN; HERMES ANTIGEN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERCELLULAR ADHESION MOLECULE 1; LYMPHOCYTE FUNCTION ASSOCIATED ANTIGEN 1; MAJOR HISTOCOMPATIBILITY ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; TRANSCRIPTION FACTOR AP 1; TRANSCRIPTION FACTOR NFAT; ANTIGEN; DNA BINDING PROTEIN; MEMBRANE ANTIGEN; NUCLEAR PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL CELL; ANTIGEN EXPRESSION; ANTIGEN PRESENTING CELL; ARTICLE; B LYMPHOCYTE; CALCIUM CELL LEVEL; CALCIUM SIGNALING; COCULTURE; CONTROLLED STUDY; FLOW CYTOMETRY; LYMPHOMA CELL; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE ACTIVATION; TRANSCRIPTION INITIATION; ANIMAL; BAGG ALBINO MOUSE; CELL COMMUNICATION; CELL CULTURE; CYTOLOGY; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; METABOLISM; METHODOLOGY; MOUSE; PHYSIOLOGY; T LYMPHOCYTE;

ANIMALS; ANTIGEN-PRESENTING CELLS; ANTIGENS; ANTIGENS, CD28; ANTIGENS, CD80; ANTIGENS, SURFACE; CALCIUM; CALCIUM SIGNALING; CELL COMMUNICATION; DNA-BINDING PROTEINS; FLOW CYTOMETRY; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED BALB C; NF-KAPPA B; NFATC TRANSCRIPTION FACTORS; NUCLEAR PROTEINS; T-LYMPHOCYTES; TRANSCRIPTION FACTOR AP-1; TRANSCRIPTION FACTORS; TUMOR CELLS, CULTURED;

EID: 0036525913     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.10086     Document Type: Article

References (37)

1. Three-dimensional segregation of supramolecular activation clusters in T cells
2. The immunological synapse: A molecular machine controlling T cell activation
3. Differential clustering of CD4 and CD3z during T cell activation
4. Signaling takes shape in the immune system
5. Serial triggering of many T-cell receptors by a few peptide-MHC complexes
6. T cell activation determined by T cell receptor number and tunable thresholds
7. The actin cytoskeleton and lymphocyte activation
8. The immunological synapse and the actin cytoskeleton: Molecular hardware for T cell signaling
9. A receptor/cytoskeletal movement triggered by costimulation during T cell activation
10. T lymphocyte costimulation mediated by reorganization of membrane microdomains
11. Distinct roles for LFA-1 and CD28 during activation of naive T cells: Adhesion versus costimulation
12. T cell co-stimulatory molecules other than CD28
13. CD28/B7 system of T cell costimulation
14. T cell responses are governed by avidity and costimulatory thresholds
15. Duration of TCR stimulation determines costimulatory requirement of T cells
16. Naive and effector CD4 T cells differ in their requirements for T cell receptor versus costimulatory signals
17. Polarity of T cell shape, motility, and sensitivity to antigen
18. Imaging early steps of human T cell activation by antigen-presenting cell
19. Differential activation of transcription factors induced by Ca2+ response amplitude and duration
20. Calcium oscillations increase the efficiency and specificity of gene expression
21. Cell-permeant caged InsP3 ester shows that Ca2+ spike frequency can optimize gene expression
22. Distinct signal transduction in mouse CD4+ and CD8+ splenic T cells after CD28 receptor ligation
23. Regulating T helper cell immunity through antigen responsiveness and calcium entry
24. Clone-specific T cell receptor antagonists of major histocompatibility complex class I-restricted cytotoxic T cells
25. Collaboration of TCR-, CD4- and CD28-mediated signalling in antigen-specific MHC class II-restricted T-cells
26. Two separate mechanisms of T cell clonal anergy to Mls-1a1
27. Signal extinction and T cell repolarization in T helper cell-antigen-presenting cell conjugates
28. Establishment of B cell hybridomas with B cell surface antigens
29. I-region restricted antigen presentation by B cell-B lymphoma hybridomas
30. T cell recognition of the posttranlationally cleaved intersubunit region of influenza virus hemagglutinin
31. Mapping of a protective helper T cell epitope of human influenza A virus hemagglutinin
32. Characterizing immunodominant and protective influenza hemagglutinin epitopes by functional activity and relative binding to major histocompatibility complex class II sites
33. Isolation of intact nuclei from hematopoietic cell types
34. Stimulation of the Ca2+-mediated egr-1 and c-fos expression in murine erythroleukemia cells by cyclosporin A
35. CD28 affects the earliest signaling events generated by TCR engagement
36. CTLA-4 ligation suppresses CD28-induced NF-kB and AP-1 activity in mouse T cell blast
37. Autonomous induction of proliferation, JNK and NF-kB activation in primary resting T cells by mobilized CD28