Identification of serine138 residue in the 4-residue segment k135k136i137s138 of luks-i component of staphylococcus intermedius leukocidin crucial for the luks-i-specific function of staphylococcal leukocidin

Bioscience, Biotechnology and Biochemistry

Volumn 66, Issue 2, 2002, Pages 328-335

  Nishiyama, Akihito ^a   Guerra, Marie Antonette Ruth V ^a,b   Sugawara, Noriko ^a   Yokota, Kenji ^a   Kaneko, Jun ^a   Kamio, Yoshiyuki ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b UNIVERSITY OF THE PHILIPPINES LOS BAÑOS   (Philippines)

Author keywords

LukF I; LukS I; Protein kinase A inhibitor H 89; Staphylococcal leukocidin; Staphylococcus intermedius

Indexed keywords

BACTERIA (MICROORGANISMS); ORYCTOLAGUS CUNICULUS; STAPHYLOCOCCUS; STAPHYLOCOCCUS INTERMEDIUS;

BACTERIAL PROTEIN; CYCLIC AMP DEPENDENT PROTEIN KINASE; LEUKOCIDIN; LEUKOCIDIN S COMPONENT PROTEIN, STAPHYLOCOCCUS; LEUKOCIDIN S-COMPONENT PROTEIN, STAPHYLOCOCCUS; PRIMER DNA; SERINE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; DRUG EFFECT; HEMOLYSIS; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; RABBIT; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; CYCLIC AMP-DEPENDENT PROTEIN KINASES; DNA PRIMERS; HEMOLYSIS; HUMANS; LEUKOCIDINS; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; RABBITS; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; STAPHYLOCOCCUS;

EID: 0036479351     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.66.328     Document Type: Article

References (17)

1. Tomita, T. and Kamio, Y., Molecular biology of the pore-forming cytolysins from S. aureus, a- and g-hemolysins and leukocidin. Biosci. Biotechnol. Biochem., 61, 565-572 (1997).
2. Kamio, Y., Tomita, T., and Kaneko, J., Pore-forming cytolysins from Staphylococcus aureus, a- and g-hemolysins and leukocidin. Staphylococcal disease and Infection, ed. by Friedman and Kluwer, Academic /plenum Publishers, pp. 179-212 (2001).
3. Sugawara, N., Tomita, T., Sato, T., and Kamio, Y., Assembly of Staphylococcus aureus leukocidin into a pore-forming ring-shaped oligomer on human polymorphonuclear leukocytes and rabbit erythrocytes. Biosci. Biotechnol. Biochem., 63, 884-891 (1999).
4. 246 residue is a potential phosphorylated site by protein kinase A for the LukS function of staphylococcal leukocidin. FEBS Lett., 415, 96-100 (1997).
5. Nishiyama, A., Nariya, H., and Kamio, Y., Phosphorylation of LukS by protein kinase A is crucial for the LukS function of the staphylococcal leukocidin on human polymorphonuclear leukocytes. Biosci. Biotechnol. Biochem., 62, 1834-1838 (1998).
6. Prevost, G., Bouakham, T., Piemont, Y., and Monteil, H., Characterization of a synergo-hymenotropic toxin produced by Staphylococcus in-termedius. FEBS Lett., 376, 135-140 (1995).
7. Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K., and Pease, L. R., Engineering hybrid genes without the use of restriction enzymes:gene splicing by overlap extension. Gene, 77, 61-68 (1989).
8. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K., and Pease, L. R., Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene, 77, 51-59 (1989).
9. Nariya, H., Asami, I., Ozawa, T., Beppu, Y., Izaki, K., and Kamio, Y., Improved method for purification of leukocidin and g-hemolysin components from Staphylococcus aureus. Biosci. Biotechnol. Biochem., 57, 2198-2199 (1993).
10. Kaneko, J., Ozawa, T., Tomita, T., and Kamio, Y., Sequential binding of staphylococcal g-hemolysin to human erythrocytes and complex formation of the hemolysin on the cell surface. Biosci. Biotechnol. Biocnem., 61, 846-851 (1997).
11. Nariya, H., Izaki, K., and Kamio, Y., The C-terminal region of the S component of staphylococcal leu-kocidin is essential for the biological activity of the toxin. FEBS Lett., 329, 219-222 (1993).
12. Olson, R., Nariya, H., Yokota, K., Kamio, Y., and Gouaux, E., Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel. Nature Structural Biology, 6, 134-140 (1999).
13. Pedelacq, J. D., Maveyraud, L., Prevost, G., Baba-Moussa, L., Gonzalez, A., Courcelle, E., Shepard, W., Monteil, H., Samama, J. P., and Mourey, L., The structure of a Staphylococcus aureus leukocidin component (LukF-PV) reveals the fold of the water-soluble species of a family of transmembrane pore-forming toxins. Structure, 7, 277-287 (1999).
14. Chijiwa, T., Mishima, A., Hagiwara, M., Sano, M., Hayashi, K., Inoue, T., Naito, K., Toshioka, T., and Hidaka, H., Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor cyclic AMP dependent protein kinase, N-[2(p-bromocinnamylamino)ethyl]-5-isoquinoline-sulfonamide (H-89), of PC12D pheochromocytoma cells. J. Biol. Chem., 265, 5267-5272 (1990).
15. Edelman, A. M., Blumenthal, D. K., and Krebs, E. G., Protein serine/threonine kinases. Annu. Rev. Biochem., 56, 567-613 (1987).
16. Song, L., Hobaugh, M. R., Shustak, C., Cheley, S., Bayley, H., and Gouaux, E., Structure of staphylococcal a-hemolysin, a heptameric transmembrane pore. Science, 274, 1859-1866 (1996).
17. Gouaux, E., Hobaugh, M., and Song, L., a-Hemoly-sin, g-hemolysin, and leukocidin from Staphylococcus aureus: Distant in sequence but similar in structure. Protein Science, 6, 2631-2635 (1997).