Phosphorylation of luks by protein kinase a is crucial for the luks-specific function of the staphylococcal leukocidin on human polymorphonuclear leukocytes

Bioscience, Biotechnology and Biochemistry

Volumn 62, Issue 9, 1998, Pages 1834-1838

  Nishiyama, Akihito ^a   Nariya, Hirofumi ^a   Kamio, Yoshiyuki ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Bi component cytolysin; LukS; Protein kinase A inhibitor H 89; Staphylococcal leukocidin; Toxin phosphorylated by protein kinase A

Indexed keywords

BACTERIAL PROTEIN; CYCLIC AMP DEPENDENT PROTEIN KINASE; LEUKOCIDIN; LEUKOCIDIN S COMPONENT PROTEIN, STAPHYLOCOCCUS; LEUKOCIDIN S-COMPONENT PROTEIN, STAPHYLOCOCCUS;

ARTICLE; HUMAN; METABOLISM; NEUTROPHIL; PHOSPHORYLATION; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEINS; CYCLIC AMP-DEPENDENT PROTEIN KINASES; HUMANS; LEUKOCIDINS; NEUTROPHILS; PHOSPHORYLATION; STAPHYLOCOCCUS AUREUS;

EID: 0032151607     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.62.1834     Document Type: Article

References (13)

1. Tomita, T. and Kamio, Y., Molecular biology of the pore-forming cytolysins from Staphylococcus aureus, a- and y-hemolysin and leukocidin. Biosci. Biotechnol. Biochem., 61, 565-572 (1997).
2. Kamio, Y., Rahman, A., Nariya, H., Ozawa, T., and Izaki, K., The two staphylococcal bi-component toxins, leukocidin and gamma-hemolysin, share one component in common. FEBS Lett., 321,15-18 (1993).
3. Rahman, A., Izaki, K., and Kamio, Y., Gamma-hemolysin genes in the same family with lukF and lukS genes in methicillin resistant Staphylococcus aureus. Biosci. Biotechnol. Biochem., 57, 1234-1236 (1993).
4. 246 residue is a potential phosphorylated site by protein kinase A for the LukS-specific function of staphylococcal leukocidin. FEBS Lett., 415, 96-100 (1997).
5. Chijiwa, T., Mishima, A., Hagiwara, M., Sano, M., Hayashi, K., Inoue, T., Naito, K., Toshioka, T., and Hidaka, H., Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2(p-bromocinnamylamino) ethyl]-5-isoquinoline-sulfonamide (H-89), of PC12D pheo-chromocytoma cells. J. Biol. Chem., 265, 5267-5272 (1990).
6. Nariya, H. and Kamio, Y., Identification of the essential region for LukS- and Hyl I-specific function of staphylococcal leu- kocidin and y-hemolysin. Biosci. Biotechnol. Biochem., 59, 1603-1604 (1995).
7. Sudo, K., Choorit, W., Asami, I., Kaneko, J., Muramoto, K., and Kamio, Y., Infstitution of lysine for arginine in the N-temi-nal 217th amino acid residue of the Hyll of staphylococcal y- hemolysin lowers the activity of the toxin. Biosci. Biotechnol. Biochem., 59, 1786-1789 (1995).
8. Nariya, H., Asami, I., Ozawa, T., Beppu, Y., Izaki, K., and Kamio, Y., Improved method for purification of leukocidin and gamma-hemolysin components from Staphylococcus aureus. Biosci. Biotechnol. Biochem., 57, 2198-2199 (1993).
9. Nariya, H., Izaki, K. and Kamio, Y., The C-terminal region of the S component of staphylococcal leukocidin is essential for the biological activity of the toxin. FEBS Lett., 329, 219-222 (1993).
10. Song, L., Hobaugh, M. R., Shustak, C., Cheley, S, Bayley, H., and Gouaux, J. E., Structure of staphylococcal a-hemolysin, a heptameric transmembrane pore. Science, 274, 1859-1866 (1997).
11. Gouaux, J. E., Hobaugh, M., and Song, L., alpha-Hemolysin, gamma-hemolysin, and leukocidin from Staphylococcus aureus: distant in sequence but similar in structure. Protein Sci., 6, 2631-2635 (1997).
12. Noda, M., Kato, I., Hirayama, T., and Matsuda, F., Fixation and inactivation of staphylococcal leukocidin by phosphatidylcholine and ganglioside GmI in rabbit polymorphonuclear leukocytes. Infect. Immun., 29, 678-684 (1980).
13. Finck-Barbacon, V., Duportail, G., Meunier, O. and Colin, D. A., Pore formation by a two-component leukocidin from Staphylococcus aureus within the membrane of human polymorphonuclear leukocytes. Biochim. Biophys. Acta, 1182, 275-282 (1993).