Assembly of staphylococcus aureus leukocidin into a pore-forming ring-shaped oligomer on human polymorphonuclear leukocytes and rabbit erythrocytes

Bioscience, Biotechnology and Biochemistry

Volumn 63, Issue 5, 1999, Pages 884-891

  Sugawara, Noriko ^a   Tomita, Toshio ^a   Sato, Tsuruji ^a   Kamio, Yoshiyuki ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Human polymorphonuclear leukocyte; Leukocidin; Membrane pore; Rabbit erythrocyte; Ring shaped structure

Indexed keywords

LEUKOCIDIN;

ANIMAL; ARTICLE; BLOOD; CELL MEMBRANE; CHEMISTRY; ELECTRON MICROSCOPY; ERYTHROCYTE; HUMAN; NEUTROPHIL; RABBIT; STAPHYLOCOCCUS AUREUS; ULTRASTRUCTURE;

ANIMALS; CELL MEMBRANE; ERYTHROCYTES; HUMANS; LEUKOCIDINS; MICROSCOPY, ELECTRON; NEUTROPHILS; RABBITS; STAPHYLOCOCCUS AUREUS;

EID: 0033125070     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.63.884     Document Type: Article

References (19)

1. Tomita T. and Kamio, Y., Molecular biology of the pore-forming cytolysins from Staphylococcus aureus, a- and y-hemolysins and leukocidin. Biosci. Biotechnol. Biochem., 61, 565-572 (1997).
2. Woodin, A. M., Purification of the two components of leukocidin from Staphylococcus aureus. Biochem. J., 75, 158-165 (1960).
3. Noda, M., Kato, I., Hirayama, T., and Matsuda, F., Crystallization and properties of staphylococcal leukocidin. Biochim. Biophys. Acta, 633, 33-44 (1980).
4. Noda, M., Hirayama, T., Kato, I., and Matsuda, F., Fixation and inactivation of staphylococcal leukocidin by phosphatidylcholine and ganglioside GM1 in rabbit polymorphonuclear leukocytes. Infect. Immun., 29, 678-684 (1980).
5. Finck-Barbancon, V., Prevost, G., and Piemont, Y., Improved purification of leukocidin from Staphylococcus aureus and toxin distribution among hospital strains. Res. Microbiol., 142, 75-85 (1991).
6. Nariya, H., Asami, I., Ozawa, T., Beppu, Y., Izaki, K., and Kamio, Y., Improved method for purification of leukocidin and gamma-hemolysin components from Staphylococcus aureus. Biosci. Biotechnol. Biochem., 57, 2198-2199 (1993).
7. Colin, D. A., Mazurier, I., Sire, S., Finck-Barbancon, V. Interaction of the two components of leukocidin from Staphylococcus aureus human polymorphonuclear leukocytes membranes; Sequential binding and subsequent activation. Infect. Immun., 62, 3184-3188.
8. Nariya, H., Nishiyama, A., and Kamio, Y. Identification of the minimum segment in which the threonine246 residue is a potential phosphorylated site by protein kinase A for the lukS function of staphylococcal leukocidin. FEBS Lett., 415, 96-100 (1997).
9. Nishiyama, A., Nariya, H., and Kamio, Y. Phosphorylation of LukS by protein kinase A is crucial for the LukS function of the staphylococcal leukocidin on human polymorphonuclear leukocytes. Biosci. Biotechnol. Biochem., 62, 1834-1838 (1998).
10. Finck-Barbancon, V., Duportail, G., Meunier, O., and Colin, D. A., Pore formation by a two-component leukocidin from Staphylococcus aureus within the membrane of human polymorphonuclear leukocytes. Biochim. Biophys. Acta, 1182, 275-282 (1993).
11. 2+channels in the membrane of human polymorphonuclear neutrophils. J. Membr. Biol., 162, 209-216 (1998).
12. Prevost, G., Cribier, B., Couppie, P., Petiau, P., Super sac, G., Finck-Barbancon, V., Monteil, H., and Piemont, Y., Panton-Valentine leukocidin and y-hemolysin from Staphylococcus aureus ATCC 49775 are encoded by distinct genetic loci and have different biological activities. Infect. Immun., 63, 4121-4129 (1995).
13. Nariya, H., Shimatani, A., Tomita, T., and Kamio, Y., Identification of the essential amino acid residues in LukS for the hemolytic activity of staphylococcal leukocidin towards rabbit erythrocytes. Biosci. Biotechnol. Biochem., 61, 2095-2099 (1997).
14. Sugawara, N., Tomita, T., and Kamio, Y., Assembly of Staphylococcus aureus y-hemolysin into a pore-forming ring-shaped complex on the surface of human erythrocytes. FEBS Lett., 410, 333-337 (1997).
15. Tomita, T., Ishikawa, D., Noguchi, T., Katayama, E., and Hashimoto, Y. Assembly of flammutoxin, a cytolytic protein from the edible mushroom Flammulina velutipes, into a poreforming ring-shaped oligomer on the target cell. Biochem. J., 333, 129-137 (1998).
16. Scherrer, R. and Gerhardt, P., Molecular sieving by the Bacillus megaterium cell wall and protoplast. J. Bacteriol., 107, 718-735 (1971).
17. Sabirov, R. Z., Krasilnikov, O. V., Ternovsky, V. I., and Merziliak, P. G., Relation between ion channel conductance and conductivity of media containing different nonelectrolytes. A novel mothod of pore size determination. Gen. Physiol. Biophys., 12, 95-111 (1993).
18. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
19. Freedman, J. C. and Hoffman, J. F., Ionic and osmotic equilibria of human red blood cells treated with nystatin. J. Gen. Physiol, 74, 157-185 (1979).