Convulxin induces platelet shape change through myosin light chain kinase and Rho kinase

European Journal of Biochemistry

Volumn 269, Issue 23, 2002, Pages 5878-5884

  Riondino, Silvia ^a   Gazzaniga, Pier Paolo ^a   Pulcinelli, Fabio M ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Convulxin; Cyclic AMP; Myosin light chain kinase; Platelet shape change; Rho kinase

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; CALCIUM ION; CALMODULIN; CONVULXIN; CYCLIC AMP; ETHYLENE GLYCOL 1,2 BIS(2 AMINOPHENYL) ETHER N,N,N',N' TETRAACETIC ACID; FASUDIL; ILOPROST; MYOSIN LIGHT CHAIN KINASE; N (6 AMINOHEXYL) 5 CHLORO 1 NAPHTHALENESULFONAMIDE; RHO KINASE;

ARTICLE; CELL STIMULATION; ENZYME PHOSPHORYLATION; HUMAN; INHIBITION KINETICS; NORMAL HUMAN; PRIORITY JOURNAL; THROMBOCYTE ACTIVATION; THROMBOCYTE SHAPE;

BLOOD PLATELETS; CALCIUM; CROTALID VENOMS; EGTAZIC ACID; HUMANS; ILOPROST; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; LECTINS, C-TYPE; MYOSIN LIGHT CHAINS; MYOSIN-LIGHT-CHAIN KINASE; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; SULFONAMIDES;

EID: 0036447454     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.03305.x     Document Type: Article

References (31)

1. Siess, W. (1989) Molecular mechanisms of platelet activation. Physiol. Rev. 69, 58-178.
2. Daniel, J.L., Molish, I.R., Rigmaiden, M. & Stewart, G. (1984) Evidence for a role of myosin phosphorylation in the initiation of the platelet shape change response. J. Biol. Chem. 259, 9826-9831.
3. Hathaway, D.R., Eaton, C.R. & Adelstein, R.S. (1981) Regulation of human platelet myosin light chain kinase by the catalytic subunit of cyclic AMP-dependent protein kinase. Nature 291, 252-256.
4. Klages, B., Brandt, U., Simon, M.I., Schultz, G. & Offermanns, S. (1999) Activation of G12/G13 results in shape change and Rho/Rho-kinase-mediated myosin light chain phosphorylation in mouse platelets. J. Cell. Biol. 144, 745-754.
5. Bauer, M., Retzer, M., Wilde, J.L., Maschberger, P., Essler, M., Aepfelbacher, M., Watson, S.P. & Siess, W. (1999) Dichotomous regulation of myosin phosphorylation and shape change by Rhokinase and calcium in intact human platelets. Blood 94, 1665-1672.
6. Paul, B.Z., Daniel, J.L. & Kunapuli, S.P. (1999) Platelet shape change is mediated by both calcium-dependent and -independent signaling pathways. Role of p160 Rho-associated coiled-coil-containing protein kinase in platelet shape change. J. Biol. Chem. 274, 28293-28300.
7. Offermanns, S., Toombs, C.F., Hu, Y.H. & Simon, M.I. (1997) Defective platelet activation in Gα(q)-deficient mice. Nature 389, 183-186.
8. Lamb, N.J., Fernandez, A., Conti, M.A., Adelstein, R., Glass, D.B., Welch, W.J. & Feramisco, J.R. (1988) Regulation of actin microfilament integrity in living nonmuscle cells by the cAMP-dependent protein kinase and the myosin light chain kinase. J. Cell. Biol. 106, 1955-1971.
9. Glass, W.F. & Kreisberg, J.I. (1993) Regulation of integrin-mediated adhesion at focal contacts by cyclic AMP. J. Cell. Physiol. 157, 296-306.
10. Han, J.D. & Rubin, C.S. (1996) Regulation of cytoskeleton organization and paxillin dephosphorylation by cAMP. Studies on murine Y1 adrenal cells. J. Biol. Chem. 271, 29211-29215.
11. Troyer, D.A., Bouton, A., Bedolla, R. & Padilla, R. (1996) Tyrosine phosphorylation of focal adhesion kinase (p125FAK): Regulation by cAMP and thrombin in mesangial cells. J. Am. Soc. Nephrol. 7, 415-423.
12. Riondino, S., Gazzaniga, P.P. & Pulcinelli, F.M. (2001) Evidence for platelet αIIbβ3 activation despite elevated cytosolic cAMP. Thromb. Haemost. 85, 320-325.
13. Polgar, J., Clemetson, J.M., Keherel, B.E., Wiedemann, M., Magnenat, E.M., Wells, T.N.C. & Clemetson, K.J. (1997) Platelet activation and signal transduction by convulxin, a C-type lectin from Crotalus durissus terrificus (tropical rattlesnake) venom via the p62/GPVI collagen receptor. J. Biol. Chem. 272, 13576-13583.
14. Faili, A., Randon, J., Francischetti, I.M., Vargaftig, B.B. & Hatmi, M. (1994) Convulxin-induced platelet aggregation is accompanied by a powerful activation of the phospholipase C pathway. Biochem. J. 298, 87-91.
15. Asazuma, N., Wilde, J.W., Berlanga, O., Leduc, M., Leo, A., Schweighoffer, E., Tybulewicz, V., Bon, C., Liu, S.K., McGlade, J., Schraven, B. & Watson, S.P. (2000) Interaction of linker for activation of T cells with multiple adapter proteins in platelets activated by the glycoprotein VI-selective ligand, convulxin. J. Bio. Chem. 275, 33427-33434.
16. Francischetti, I.M.B., Carlini, R. & Guimaraes, J.A. (1998) cAMP does not inhibit convulxin-induced tyrosyl-phosphorylation of human platelet proteins, including PLCγ2, but completely blocks the integrin αIIbβ3-dependent dephosphorylation step: Comparisons with RGDS peptide, cytochalasin D, and phenylarsine oxide. Arch. Biochem. Biophys. 354, 255-269.
17. Aster, R.H. & Jandel, J.H. (1964) Platelet sequestration in man: I: Methods. J. Clin. Invest. 43, 834-855.
18. Levin, R.M. & Weiss, B. (1979) Selective binding of antipsychotics and other psychoactive agents to the calcium-dependent activator of cyclic nucleotide phosphodiesterase. J. Pharmacol. Exp. Ther. 208, 454-459.
19. Hidaka, H., Asano, M. & Tanaka, T. (1981) Activity-structure relationship of calmodulin antagonists, Naphthalenesulfonamide derivatives. Mol. Pharmacol. 20, 571-578.
20. Takayasu, M., Suzuki, Y., Shibuya, M., Asano, T., Kanamori, M., Okada, T., Kageyama, N. & Hidaka, H. (1986) The effects of HA compound calcium antagonists on delayed cerebral vasospasm in dogs. J. Neurosurg. 65, 80-85.
21. Uehata, M., Ishizaki, T., Satoh, H., Ono, T., Kawahara, T., Morishita, T., Tamakawa, H., Yamagami, K., Inui, J., Maekawa, M. & Narumiya, S. (1997) Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature 389, 990-994.
22. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260, 3440-3450.
23. Daniel, J.L. & Sellers, J.R. (1992) Purification and characterization of platelet myosin. Methods Enzymol. 215, 78-88.
24. Daniel, J.L., Molish, I.R. & Holmsen, H. (1981) Myosin phosphorylation in intact platelets. J. Biol. Chem. 256, 7510-7514.
25. 2+ thresholds and Ca-independent activation for shape-change and secretion. FEBS Lett. 148, 21-26.
26. 2+]i. FEBS Lett. 201, 301-305.
27. Negrescu, E.V., de Quintana, K.L. & Siess, W. (1995) Platelet shape change induced by thrombin receptor activation. Rapid stimulation of tyrosine phosphorylation of novel protein substrates through an integrin- and Ca (2+) -independent mechanism. J. Biol. Chem. 270, 1057-1061.
28. Wilde, J.I., Retzer, M., Siess, W. & Watson, S.P. (2000) ADP-induced platelet shape change. An investigation of the signalling pathways involved and their dependence on the method of platelet preparation. Platelets 11, 286-295.
29. Lampugnani, M.G., Giorgi, M., Gaboli, M., Dejana, E. & Marchisio, P.C. (1990) Endothelial cell motility, integrin receptor clustering, and microfilament organization are inhibited by agents that increase intracellular cAMP. Laboratory Invest. 63, 521-531.
30. Gachet, C. (2001) ADP receptors of platelets and their inhibition. Thromb Haemost 86, 222-232.
31. 2+ release from platelet membranes. Biochem. Biophys. Res. Commun. 184, 893-899.