Purification of overproduced Escherichia coli RNA polymerase σ factors by solubilizing inclusion bodies and refolding from Sarkosyl

Methods in Enzymology

Volumn 273, Issue , 1996, Pages 145-149

  Burgess, Richard R ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE; SIGMA FACTOR;

ARTICLE; CONFORMATIONAL TRANSITION; ENZYME CONFORMATION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PURIFICATION; RECOMBINANT DNA TECHNOLOGY; SOLUBILIZATION;

EID: 0029822194     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0076-6879(96)73014-8     Document Type: Article

References (7)

1. C. H. Schein, Bio/Technology 7, 1141 (1989).
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4. L. H. Nguyen, D. B. Jensen, and R. R. Burgess, Protein Expr. Purif. 4, 425 (1993).
5. L. H. Nguyen, D. B. Jensen, N. E. Thompson, D. R. Gentry, and R. R. Burgess, Biochemistry 32, 11112 (1993).
6. D. Marshak, J. Kadonaga, R. Burgess, M. Knuth, S.-H. Lin, and W. Brennan, "Strategies for Protein Purification and Characterization: A Laboratory Manual." pp. 205-274. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1996.
7. M. Ouhammouch, K. Adelman, S. R. Harvey, G. Orsini, and E. N. Brody, Proc. Natl. Acad. Sci. U.S.A. 92, 1 (1995).