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In redox chemistry, the oxidized component is listed first, i.e., the GSH/GSSG couple is expressed correctly as the GSSG/GSH couple. However, because of the common use of the term "GSH/GSSG" in biochemical and health-related literature, this tradition of expressing donor/acceptor is followed in reference to thiol-zlisulfide redox couples even though other terms and definitions are those used conventionally for redox chemistry.
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Perchloric acid was used for precipitation of protein because perchlorate is readily precipitated as the potassium salt during adjustment of pH to 9.0. Removal of the anion is useful because derivatives are eluted from the cationic HPLC column by an anion gradient. Thus, variation in injection volume of samples with a high anion concentration can affect retention times.
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13
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85030747723
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note
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Carboxymethylation is about 80% complete after 2 min in the original collection of plasma in the preservation solution. In principle, one could allow this reaction to proceed to completion prior to transfer of plasma from tube A to the perchloric acid solution in tube B. This approach was not pursued in the current development because it would require more precise timing for the collection procedure.
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