Structure of porphobilinogen synthase from pseudomonas aeruginosa in complex with 5-fluorolevulinic acid suggests a double schiff base mechanism

Journal of Molecular Biology

Volumn 320, Issue 2, 2002, Pages 237-247

  Frère, Frederic ^a   Schubert, Wolf Dieter ^b   Stauffer, Frédéric ^c   Frankenberg, Nicole ^d   Neier, Reinhard ^c   Jahn, Dieter ^a   Heinz, Dirk W ^b  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c UNIVERSITY OF NEUCHÂTEL   (Switzerland)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

5 fluorolevulinic acid; Crystal structure; Enzyme inhibitor complex; Porphobilinogen synthase; Pseudomonas aeruginosa

Indexed keywords

5 FLUOROLEVULINIC ACID; LEVULINIC ACID; PORPHOBILINOGEN SYNTHASE; SCHIFF BASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CATALYSIS; COMPLEX FORMATION; CRYSTAL STRUCTURE; DATA ANALYSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0036299038     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00472-2     Document Type: Article

References (31)

1. Chemical synthesis of porphobilinogen and studies of its biosynthesis
2. X-ray structure of 5-aminolevulinic acid dehydratase from Escherichia coli complexed with the inhibitor levulinic acid at 2.0 Å resolution
3. Mechanistic basis for suicide inactivation of porphobilinogen synthase by 4,7-dioxo sebacic acid, an inhibitor that shows dramatic species selectivity
4. The X-ray structure of yeast 5-aminolaevulinic acid dehydratase complexed with two diacid inhibitors
5. Bradyrhizobium japonicum porphobilinogen synthase uses two Mg(II) and monovalent cations
6. Comparative studies on the 5-aminolaevulinic acid dehydratases from Pisum sativum, Escherichia coli and Saccharomyces cerevisiae
7. Cloning and characterisation of the Pseudomonas aeruginosa hemB encoding a magnesium-dependent aminolevulinic acid dehydratase
8. 2+-responsive porphobilinogen synthase from Pseudomonas aeruginosa
9. High-resolution crystal structure of a magnesium-dependent 5-aminolevulinic acid dehydratase
10. The porphobilinogen synthase family of metalloenzymes
11. Delta-aminolevulinic acid dehydratase of Rhodospeudomonas spheroides II. Association to polymers and dissociation to subunits
12. Pseudomonas aeruginosa contains a novel type V porphobilinogen synthase with no required catalytic metal ions
13. X-ray structure of 5-aminolaevulinate dehydratase, a hybrid aldolase
14. The Schiff base complex of yeast 5-aminolaevulinic acid dehydratase with laevulinic acid
15. The structure and evolution of alpha/beta barrel proteins
16. The X-ray structure of yeast 5-aminolaevulinic acid dehydratase complexed with substrate and three inhibitors
17. Identification of lysine at the active site of human 5-aminolaevulinate dehydratase
18. Dissection of the early steps in the porphobilinogen synthase catalyzed reaction. Requirements for Schiff's base formation
19. Characterization of the two 5-aminolevulinic acid binding sites, the A-and P-sites, of 5-aminolaevulinic acid dehydratase from Escherichia coli
20. + in pyridoxal phosphate enzyme catalysis
21. Dose-response curves of enzyme inhibition
22. A new software system for macromolecular structure determination
23. Improved methods for building protein models in electron density maps and the location of errors in these models
24. Refinement of macromolecular structures by the maximum-likelihood method
25. Automated protein model building combined with iterative structure refinement
26. PROCHECK: A program to check the stereochemical quality of protein structures
27. Errors in protein structures
28. Species-specific inhibition of porphobilinogen synthase by 4-oxosebacic acid
29. MOLSCRIPT: A program to produce both detailed and schematic plots or protein structures
30. Raster3D version 2.0: A program for photorealistic molecular graphics
31. Free R-value: A novel statistical quantity for assessing the accuracy of crystal structures