The unusually slow relaxation kinetics of the folding-unfolding of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus

Journal of Molecular Biology

Volumn 316, Issue 4, 2002, Pages 991-1003

  Kaushik, Jai K ^a,b   Ogasahara, Kyoko ^a   Yutani, Katsuhide ^a  

^a OSAKA UNIVERSITY   (Japan)

^b NATIONAL DAIRY RESEARCH INSTITUTE   (India)

Author keywords

Differential scanning calorimetry; Folding unfolding kinetics; Hyperthermophile; Pyrrolidone carboxyl peptidase; Thermodynamic stability

Indexed keywords

5 OXOPROLYL PEPTIDASE;

ARTICLE; CIRCULAR DICHROISM; DIFFERENTIAL SCANNING CALORIMETRY; INCUBATION TIME; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STABILITY; PYROCOCCUS FURIOSUS; SPECTROSCOPY; TEMPERATURE; THERMOPHILIC BACTERIUM;

PYROCOCCUS; PYROCOCCUS FURIOSUS;

EID: 0036290245     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.5355     Document Type: Article

References (60)

1. Structure and stability of hyperstable proteins: Glycolytic enzymes from hyperthermophilic bacterium Thermotoga maritima
2. 2.0 Å structure of indole-3-glycerolphosphatae synthase from the hyperthermophile Sulfolobus solfataricus: Possible determinants of protein stability
3. Conservation of rapid two-state folding in mesophilic, thermophilic and hyperthermophilic cold shock proteins
4. Kinetic role of electrostatic interactions in the unfolding of hyperthermophilic and mesophilic rubredoxins
5. Transition state of heat denaturation of methionine aminopeptidase from a hyperthermophile
6. Extremely thermostable D-glyceraldehyde-3-phosphate dehydrogenase from the eubacterium Thermotoga maritima
7. Glutamate dehydrogenase from the hyperthermophile Pyrococcus furiosus. Thermal denaturation and activation
8. The purification and characterization of an extremely thermostable α-amylase from the hyperthermophilic archaebacterium Pyrococcus furiosus
9. Equilibrium DNA binding of Sac7d protein from the hyperthermophile Sulfolobus acido-caldarius: Fluorescence and circular dichroism studies
10. The crystal structure of a hyperthermophilic archaeal TATA-box binding protein
11. Ferredoxin from the hyperthermophile Thermotoga maritima is stable beyond the boiling point of water
12. Disruption of an ionic network leads to accelerated thermal denaturation of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima
13. Electrostatic stabilization in methionine aminopeptidase from hyperthermophile Pyrococcus furiosus
14. Reversible thermal unfolding of thermostable cytochrome c-552
15. Hyperthermophile protein folding thermodynamics: Differential scanning calorimetry and chemical denaturation of Sac7d
16. Thermal unfolding of the DNA-binding protein Sso7d from the hyperthermophile Sulfolobus solfataricus
17. Thermal unfolding and conformational stability of the recombinant domain II of glutamate dehydrogenase from the hyperthermophile Thermotoga maritima
18. Slow unfolding and refolding kinetics of the mesophilic Rop wild-type protein in the transition range
19. Dimer-to-tetramer transformation: Loop excision dramatically alters structure and stability of the ROP four α-helix bundle protein
20. Refolding studies on the tetrameric loop deletion mutant RM6 of ROP protein
21. Pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Pyrococcus furiosus: Cloning and overexpression in Escherichia coli of the gene, and its application to protein sequence analysis
22. X-ray crystalline structures of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus, and its Cys-free mutant
23. Thermal stability of pyrrolidone carboxyl peptidases from the hyperthermophilic Archaeon, Pyrococcus furiosus
24. Influence of transition rates and scan rate on kinetic simulations of differential scanning calorimetry profiles of reversible and irreversible protein denaturation
25. Folding under inequilibrium conditions as a possible reason for partial irreversibility of irreversibility of heat-denatured proteins: Computer simulation study
26. The unusually slow unfolding rate causes the high stability of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus: Equilibrium and kinetic studies of guanidine hydrochloride-induced unfolding and refolding
27. How do small single-domain proteins fold?
28. Lower kinetic limit to protein thermal stability: A proposal regarding protein stability in vivo and its relation with misfolding diseases
29. Stability of proteins in extreme environments
30. Contact order, transition state placement and the refolding rates of single domain proteins
31. Single versus parallel pathways of protein folding and fractional formation of structure in the transition state
32. Cytochrome-c folding triggered by electron-transfer
33. The energy landscape of a fast-folding protein mapped by Ala → Gly substitutions
34. Topology, stability, sequence, and length: Defining the determinants of two-state protein folding kinetics
35. Denaturant m values and heat capacity changes: Relation to changes in accessible surface areas of protein unfolding
36. Is the hydrophobic effect stabilizing or destabilizing in proteins? The contribution of disulphide bonds to protein stability
37. Heat capacity and conformation of proteins in the denatured state
38. Comparing the thermodynamic stabilities of a related thermophilic and mesophilic enzyme
39. Stability and folding of dihydrofolate reductase from the hyperthermophilic bacterium Thermotoga maritima
40. Protein stability and molecular adaptation to extreme conditions
41. Dependence of protein stability on the structure of the denatured state: Free energy calculations of I56V mutation in human lysozyme
42. Entropic stabilization of the tryptophan synthase α-subunit from a hyperthermophile, Pyrococcus furiosus: X-ray analysis and calorimetry
43. Insights into the molecular basis of thermal stability from the analysis of ion-pair networks in the glutamate dehydrogenase family
44. Are buried salt bridges important for protein stability and conformational specificity?
45. Protein stabilization by removal of unsatisfied polar groups: Computational approaches and experimental tests
46. Buried charged surface in proteins
47. Structural basis for thermostability and identification of potential active site residues for adenylate kinases from the archaeal genus Methanococcus
48. Thermal adaptation analyzed by comparison of protein sequences from mesophilic and extremely thermophilic Methanococcus species
49. The refined crystal structure of Bacillus cereus oligo-1,6-glucosidase at 2.0 Å resolution: Structural characterization of proline-substitution sites for protein thermostabilization
50. Enhanced thermal stability of Clostridium beijerinckii alcohol dehydrogenase after strategic substitution of amino acid residues with prolines from the homologous thermophilic Thermoanaerobacter brockii alcohol dehydrogenase
51. Factors enhancing protein thermostability
52. Submillisecond folding of monomeric λ repressor
53. Preorganized secondary structure as an important determinant of fast protein folding
54. Slow folding of muscle acylphosphatase in the absence of intermediates
55. The development of tertiary interactions during the folding of a large protein
56. Melting-profile analysis of thermal stability of thermolysin. A formulation of temperature-scanning kinetics
57. Thermal denaturation of the Ca2()-ATPase of sarcoplasmic reticulum reveals two thermodynamically independent domains
58. Thermal stability of proteins in aqueous polyol solutions: Role of surface tension of water in the stabilizing effect of polyols
59. A mechanistic analysis of the increase in the thermal stability of proteins in aqueous carboxyl acid salt solutions