Time-sensitive reversal of hyperplasia in transgenic mice expressing SV40 T antigen

Science

Volumn 273, Issue 5280, 1996, Pages 1384-1386

  Ewald, Dagmar ^a   Li, Minglin ^b   Efrat, Shimon ^c   Auer, Gert ^d   Wall, Robert J ^e   Furth, Priscilla A ^b   Hennighausen, Lothar ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^c ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^d KAROLINSKA INSTITUTE   (Sweden)

^e AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS T ANTIGEN;

ANIMAL CELL; ANTIGEN EXPRESSION; ARTICLE; CARCINOGENESIS; CELL TRANSFORMATION; HYPERPLASIA; MALIGNANT NEOPLASTIC DISEASE; MALIGNANT TRANSFORMATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SIMIAN VIRUS 40; SUBMANDIBULAR GLAND; TRANSGENIC MOUSE; VIRUS EXPRESSION;

EID: 0029818753     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5280.1384     Document Type: Article

References (26)

1. R. A. Weinberg, Ann. N. Y. Acad Sci. 758, 331 (1995).
2. E. Sinn et al., Cell 49, 465 (1987).
3. W. J. Muller, E. Sinn, P. K. Pattengale, R. Wallace, P. Leder, ibid. 54, 105 (1988).
4. T. A. Stewart, P. K. Pattengale, P. Leder, ibid. 38, 627 (1984).
5. L. A. Donehower et al., Genes Dev. 9, 882 (1995).
6. A. Elson, C. Deng, J. Campos-Torres, L. A. Donehower, P. Leder, Oncogene 11, 181 (1995).
7. M. Gossen and H. Bujard, Proc. Natl. Acad. Sci. U.S.A. 89, 5547 (1992).
8. P. A. Furth et al., ibid. 91, 9302 (1994).
9. L. Hennighausen, R. Wall, U. Tillmann, M. Li, P. A. Furth, J. Cell. Biochem. 59, 463 (1995).
10. J. A. DeCaprio et al., Cell 54, 275 (1988).
11. D. P. Lane and L. V. Crawford, Nature 278, 261 (1979).
12. D. I. H. Linzer and A. J. Levine, Cell 17, 43 (1979).
13. J. M. Adams and S. Cory, Science 254, 1161 (1991).
14. N. M. Greenberg et al., Proc. Natl. Acad. Sci. U.S.A. 92, 3439 (1995).
15. M. Li, J. Hu, K. Heermeier, L. Hennighausen, P. A. Furth, Cell Growth Differ. 7, 3 (1996).
16. J. C. Pascall, M. A. Surani, S. C. Barton, T. J. Vaughan, K. D. Brown, J. Mol. Endocrinol. 12, 313 (1994).
17. H Symonds et al., Cell 78, 703 (1994).
18. Y. J. Tzeng, E. Guhl, M. Graessmann, A. Graessmann, Oncogene 8, 1965 (1993).
19. S. Efrat, D. Fusco-DeMane, H. Lemberg, O. Al Emran, X. Wang, Proc. Natl. Acad. Sci. U.S.A. 92, 3576 (1995).
20. Salivary gland tissue was homogenized in 1% NP-40, 0.5% sodium deoxycholate, 01% SDS, phenylmethylsulfonyl fluoride (10 mg/ml), aprotinin (30 μl/ ml) (Sigma), and 100 mM sodium orthovanadate in phosphate buffered saline. Proteins (20 μg) were separated in an 8% SDS-polyacrylamide gel and transferred onto a NOVEX polyvinylidene diflouride membrane by means of a NOVEX Western Transfer Apparatus. After transfer and blocking with buffer [5% nonfat milk, 10 mM tris-HCI (pH 7.5), 100 mM NaCI, and 0.1% Tween-20] for 1 hour at room temperature, the membrane was exposed to a 1:400 dilution of mouse monoclonal antibody Pab 101 (Santa Cruz Biotechnology, Santa Cruz, CA) followed by exposure to a 1:4000 dilution of peroxidase-conjugated rabbit polyclonal antibody to mouse immunoglobulin G (Jackson Immunochemical, West Grove, PA) for 1 hour at room temperature. The proteins were visualized with the ECL protein immunoblotting protocol (Amersham).
21. Slow-release tetracycline pellets (Innovative Research of America, Toledo, OH) were implanted sub-cutaneously in the shoulder region with the use of a trochar according to the manufacturer's directions. These pellets released 0.7 mg of tetracycline hydrochloride per day over a 21-day period.
22. P. A. Furth, M. Li, L. Hennighausen, unpublished data.
23. P. A. Furth and L, Hennighausen, unpublished data. Quantative analyses on two mice revealed that approximately 40% of the ducts were hyperplaslic.
24. The degree of ploidy was determined on Feulgenstained paraffin sections.
25. L. Hennighausen and G. Auer, unpublished data.
26. We thank G. Robinson for critical reading of the manuscript and K. Heermeier, X. Liu, K.-U. Wagner, and J. Hu for technical advice. Supported in part by a Pangborn Award from the University of Maryland Medical School (to P.A.F.).