A catalytically active group II intron domain 5 can function in the U12-dependent spliceosome

Molecular Cell

Volumn 9, Issue 5, 2002, Pages 1145-1150

  Shukla, Girish C ^a   Padgett, Richard A ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOZYME;

ARTICLE; CATALYST; IN VIVO STUDY; INTRON; METAL BINDING; PROTEIN DOMAIN; SPLICEOSOME;

EID: 0036285645     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(02)00505-1     Document Type: Article

References (32)

1. Catalytic role of 2′-hydroxyl groups within a group II intron active site
2. Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro
3. A tertiary interaction that links active-site domains to the 5′ splice site of a group II intron
4. The generality of self-splicing RNA: Relationship to nuclear mRNA splicing
5. Frequent use of the same tertiary motif by self-folding RNAs
6. Tight binding of the 5′ exon to domain I of a group II self-splicing intron requires completion of the intron active site
7. A group II self-splicing intron from the brown alga Pylaiella littoralis is active at unusually low magnesium concentrations and forms populations of molecules with a uniform conformation
8. Differential chemical probing of a group II self-splicing intron identifies bases involved in tertiary interactions and supports an alternative secondary structure model of domain V
9. Metal ion coordination by the AGC triad in domain 5 contributes to group II intron catalysis
10. Trans-activation of group II intron splicing by nuclear U5 snRNA
11. Base pairing with U6atac snRNA is required for 5′ splice site activation of U12-dependent introns in vivo
12. Group II intron domain 5 facilitates a trans-splicing reaction
13. U11 snRNA interacts in vivo with the 5′ splice site of U12-dependent (AU-AC) introns
14. Ribozyme catalysis from the major groove of group II intron domain 5
15. Comparative and functional anatomy of group II catalytic introns - A review
16. Evidence for two active sites in the spliceosome provided by stereochemistry of pre-mRNA splicing
17. The stereochemical course of group II intron self-splicing
18. Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA
19. A self-splicing RNA excises an intron lariat
20. Catalytically critical nucleotides in domain 5 of a group II intron
21. Self-splicing of group II introns in vitro: Mapping of the branch point and mutational inhibition of lariat formation
22. On the origin of RNA splicing and introns
23. "Five easy pieces"
24. Conservation of functional features of U6atac and U12 snRNAs between vertebrates and higher plants
25. The intramolecular stem-loop structure of U6 snRNA can functionally replace the U6atac snRNA stem-loop
26. Metal ion binding sites in a group II intron core
27. Metal ion catalysis during group II intron self-splicing: Parallels with the spliceosome
28. Highly diverged U4 and U6 small nuclear RNAs required for splicing rare AT-AC introns
29. A novel spliceosome containing U11, U12 and U5 snRNPs excises a minor class (AT-AC) intron in vitro
30. MRNA splicing and autocatalytic introns: Distant cousins or the products of chemical determinism?
31. Metal-ion coordination by U6 small nuclear RNA contributes to catalysis in the spliceosome
32. U6 snRNA function in nuclear pre-mRNA splicing: A phosphorothioate interference analysis of the U6 phosphate backbone