Catalytic role of 2′-hydroxyl groups within a group II intron active site

Science

Volumn 271, Issue 5254, 1996, Pages 1410-1413

  Abramovitz, Dana L ^a   Friedman, Richard A ^a,b   Pyle, Anna Marie ^a  

^a College of Physicians and Surgeons ^*  (United States)

^b New York Presbyterian Hospital Columbia University Medical Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROXYL RADICAL; OLIGORIBONUCLEOTIDE; RIBOZYME; RNA; DEOXYRIBONUCLEOTIDE; HYDROXYL GROUP;

ARTICLE; BINDING SITE; CATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; DNA BASE COMPOSITION; EXON; HYDROGEN BOND; INTRON; KINETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; COMPETITIVE INHIBITION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN DOMAIN;

BASE COMPOSITION; BASE SEQUENCE; BINDING SITES; CATALYSIS; EXONS; HYDROGEN BONDING; HYDROXYL RADICAL; INTRONS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; OLIGORIBONUCLEOTIDES; RNA; RNA, CATALYTIC;

EID: 0029670279     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5254.1410     Document Type: Article

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46. We thank B. Honig and M Akke for helpful discussion of the D5 model, S Strobel for discussion of the data and help with ligations, and Z Qin and B. Konforti for critical review of the manuscript. We also thank F. Major for use of, and help with, MC-SYM; D McKay for the coordinates of the GAAA tetraloop in the hammerhead ribozyme; and C Bingman for advice on HPLC Supported by grants GM50313 to A.M.P. and GM41371 to B. Honig from the National Institutes of Health.