Quantitative assessment of gene expression in highly purified hematopoietic cells using real-time reverse transcriptase polymerase chain reaction

Experimental Hematology

Volumn 30, Issue 5, 2002, Pages 481-487

  Raaijmakers, Marc H G P ^a   Van Emst, Liesbeth ^a   De Witte, Theo ^a   Mensink, Ewald ^a   Raymakers, Reinier A P ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CD34 ANTIGEN; CD38 ANTIGEN; COMPLEMENTARY DNA; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; LUNG RESISTANCE PROTEIN; MULTIDRUG RESISTANCE PROTEIN 1; RIBOSOME RNA;

ACCURACY; ARTICLE; CELL LINE; CELL POPULATION; CLINICAL ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; GENE EXPRESSION; HEMATOPOIETIC STEM CELL; HOUSEKEEPING GENE; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; VALIDATION PROCESS;

ADP-RIBOSYL CYCLASE; ANTIGENS, CD; ANTIGENS, CD34; ANTIGENS, CD38; ANTIGENS, DIFFERENTIATION; BONE MARROW CELLS; CELL LINE; GENE EXPRESSION REGULATION; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES, MDR; HEMATOPOIETIC STEM CELLS; HUMANS; LEUKEMIA; MEMBRANE GLYCOPROTEINS; NAD+ NUCLEOSIDASE; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY;

EID: 0036265158     PISSN: 0301472X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0301-472X(02)00787-7     Document Type: Article

References (40)

1. Extended long-term culture reveals a highly quiescent and primitive human hematopoietic progenitor population
2. Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: Implications for gene therapy
3. A cell initiating human acute myeloid leukaemia after transplantation into SCID mice
4. Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell
5. A primitive hematopoietic cell is the target for the leukemic transformation in human Philadelphia-positive acute lymphoblastic leukemia
6. Evidence for early hematopoietic progenitor cell involvement in acute promyelocytic leukemia
7. Isolation of a highly quiescent subpopulation of primitive leukemic cells in chronic myeloid leukemia
8. Recent progress in identifying genes regulating hematopoietic stem cell function and fate
9. Analysis of gene expression in a complex differentiation hierarchy by global amplification of cDNA from single cells
10. During ontogeny primitive (CD34(+) CD38(-)) hematopoietic cells show altered expression of a subset of genes associated with early cytokine and differentiation responses of their adult counterparts
11. Temporal mapping of gene expression levels during the differentiation of individual primary hematopoietic cells
12. Analysis of gene expression in subpopulations of murine hematopoietic stem and progenitor cells
13. Real time quantitative PCR
14. A novel method for real time quantitative RT-PCR
15. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
16. A low but functionally significant MDR1 expression protects primitive haemopoietic progenitor cells from anthracycline toxicity
17. +
18. Primitive human hematopoietic cells displaying differential efflux of the rhodamine 123 dye have distinct biological activities
19. Characterization of a new drug-resistant human myeloma cell line that expresses P-glycoprotein
20. Consensus strategy to quantitate malignant cells in myeloma patients is validated in a multicenter study
21. Quantitation of minimal residual disease in Philadelphia chromosome positive chronic myeloid leukaemia patients using real-time quantitative RT-PCR
22. Real-time quantitative RT-PCR after laser-assisted cell picking
23. Quality management and influential factors for the detection of single metastatic cancer cells by reverse transcriptase polymerase chain reaction
24. An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
25. Housekeeping genes as internal standards: Use and limits
26. Cell cycle regulation of the glyceraldehyde-3-phosphate dehydrogenase/uracil DNA glycosylase gene in normal human cells
27. Pancreatic GAPDH gene expression during ontogeny and acute pancreatitis induced by caerulein
28. Comparison of glyceraldehyde-3-phosphate dehydrogenase and 28S-ribosomal RNA gene expression as RNA loading controls for northern blot analysis of cell lines of varying malignant potential
29. Changes in the synthesis of ribosomal ribonucleic acid and of poly(A)-containing ribonucleic acid during the differentiation of intestinal epithelial cells in the rat and in the chick
30. Problems related to the interpretation of autoradiographic data on gene expression using common constitutive transcripts as controls
31. Expression of a gene trap reporter construct in a subset of cells in embryonic sites of hematopoiesis: Evidence for alternative rRNA production in hematopoietic cells
32. Possible defect in RNA metabolism in leukemic cells
33. Partial failure of methylation and cleavage of 45S RNA in the blast cells of acute leukaemia
34. Defective rRNA synthesis in human leukaemic blast cells?
35. Expression and activity of P-glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells
36. The transporter ABCG2 effluxes hoechst and its expression is restricted to the most primitive human hematopoietic cells
37. Overexpression of multidrug resistance-associated protein (MRP) increases resistance to natural product drugs
38. Lung resistance-related protein/major vault protein and vaults in multidrug-resistant cancer
39. P-glycoprotein plays a drug-efflux-independent role in augmenting cell survival in acute myeloblastic leukemia and is associated with modulation of a sphingomyelin-ceramide apoptotic pathway
40. Evidence that a cell-type-specific efflux pump regulates cell differentiation in Dictyostelium