Enhanced transgene expression in primitive hematopoietic progenitor cells and embryonic stem cells efficiently transduced by optimized retroviral hybrid vectors

Gene Therapy

Volumn 9, Issue 8, 2002, Pages 477-487

  Ketteler, R ^a   Glaser, S ^a   Sandra, O ^a   Martens, U M ^a   Klingmuller U ^a  

^a MAX PLANCK INSTITUTE OF IMMUNOBIOLOGY AND EPIGENETICS   (Germany)

Author keywords

Embryonic stem cells; Hybrid retroviral vector; MESV leader sequence; Primitive hematopoietic progenitor cells

Indexed keywords

GREEN FLUORESCENT PROTEIN; RETROVIRUS VECTOR;

ANIMAL CELL; ARTICLE; BINDING SITE; CONTROLLED STUDY; EMBRYO CELL; GENE EXPRESSION REGULATION; GENE INSERTION; GENE SEQUENCE; HEMATOPOIETIC STEM CELL; HEPATITIS B VIRUS; MOLONEY LEUKEMIA ONCOVIRUS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING; SPLEEN; STEM CELL; TRANSGENE; TRANSLATION REGULATION; VIRAL GENE DELIVERY SYSTEM; VIRUS HYBRID;

ANIMALIA; HEPATITIS B VIRUS; INSERTION SEQUENCES; MARMOTA MONAX; MOLONEY MURINE LEUKEMIA VIRUS; MURINAE; MURINE LEUKEMIA VIRUS; ONCOVIRINAE; SPLEEN FOCUS-FORMING VIRUS; UNIDENTIFIED RETROVIRUS; WOODCHUCK HEPATITIS B VIRUS;

EID: 0036236631     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301653     Document Type: Article

References (36)

1. Gene therapy of human severe combined immunodeficiency (SCID)-X1 disease
2. Recent developments in retroviral-mediated gene transduction
3. Applications of retrovirus-mediated expression cloning
4. Stable and functional lymphoid reconstitution of common cytokine receptor γ chain deficient mice by retroviral-mediated gene transfer
5. Novel retroviral vectors for efficient expression of the multidrug resistance (mdr-1) gene in early hematopoietic cells
6. Inactivation of a GFP retrovirus occurs at multiple levels in long-term repopulating stem cells and their differentiated progeny
7. Retroviral expression in embryonic stem cells and hematopoietic stem cells
8. Embryonic stem cell virus, a recombinant murine retrovirus with expression in embryonic stem cells
9. Characterization of the Moloney murine leukemia virus stem cell-specific repressor binding site
10. Evidence for a stem cell-specific repressor of Moloney murine leukemia virus expression in embryonal carcinoma cells
11. The potent enhancer activity of the polycythemic strain of spleen focus-forming virus in hematopoietic cells is governed by a binding site for Sp1 in the upstream control region and by a unique enhancer core exclusive target for PEBP/CBF
12. FMEV vectors: Both retroviral long terminal repeat and leader are important for high expression in transduced hematopoietic cells
13. Design of 5′ untranslated sequences in retroviral vectors developed for medical use
14. Woodchuck hepatitis virus post-transcriptional regulatory element enhances expression of transgenes delivered by retroviral vectors
15. Context dependence of different modules for post-transcriptional enhancement of gene expression from retroviral vectors
16. Increased levels of spliced RNA account for augmented expression from the MFG retroviral vector in hematopoietic cells
17. Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing
18. Production of high-titer helper-free retroviruses by transient transfection
19. Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells
20. Host cis-mediated extinction of a retrovirus permissive for expression in embryonal stem cells during differentiation
21. Increased probability of expression from modified retroviral vectors in embryonal stem cells and embryonal carcinoma cells
22. Amelioration of retroviral vector silencing in locus control region beta-globin-transgenic mice and transduced F9 embryonic cells
23. De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells
24. Complex demethylation patterns at Sp1 binding sites in F9 embryonal carcinoma cells
25. Efficient serum-free retroviral gene transfer into primitive human hematopoietic progenitor cells by a defined high-titer, nonconcentrated vector-containing medium
26. Expansion of human cord blood CD34+ CD38-cells in ex vivo culture during retroviral transduction without a corresponding increase in SCID repopulating cell (SRC) frequency: Dissociation of SRC phenotype and function
27. Retroviral gene transfer into human hematopoietic cells: An in vitro kinetic study
28. Type and position of promoter elements in retroviral vectors have substantial effects on the expression level of an enhanced green fluorescent protein reporter gene
29. CD34 splice variant: An attractive marker for selection of gene-modified cells
30. High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells
31. Influence of multiplicity of infection and protein stability on retroviral vector-mediated gene expression in hematopoietic cells
32. Dose-dependent transduction of vesicular stomatitis virus G protein-pseudotyped retrovirus vector into human solid tumor cell lines and murine fibroblasts
33. Role of cytokine signaling molecules in erythroid differentiation of mouse fetal liver hematopoietic cells: Functional analysis of signaling molecules by retrovirus-mediated expression
34. Identification of a novel pathway important for proliferation and differentiation of primary erythroid progenitors
35. Inactivation of erythropoietin leads to defects in cardiac morphogenesis
36. Functional interaction of erythropoietin and stem cell factor receptors is essential for erythroid colony formation