Human duodenal spheroids for noninvasive intracellular pH measurement and quantification of regulation mechanisms under physiological conditions

In Vitro Cellular and Developmental Biology - Animal

Volumn 38, Issue 1, 2002, Pages 7-13

  Weinlich, Michael ^a   Baumstark, Christina ^a   Usta, Engin ^a   Becker, Horst D ^a   Sessler, Michael J ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Human duodenum; Intracellular pH regulation; Spheroid; Three dimensional cell culture

Indexed keywords

4,4' DIISOTHIOCYANATOSTILBENE 2,2' DISULFONIC ACID; AMILORIDE; BICARBONATE; SODIUM; TRYPAN BLUE;

ACIDIFICATION; ARTICLE; CELL CULTURE; CELL PH; CONTROLLED STUDY; DUODENUM; FLUORESCENCE; HUMAN; HUMAN TISSUE; ION EXCHANGE; MOLECULAR PROBE; PH MEASUREMENT; PROTON SODIUM EXCHANGE; SPHEROID CELL;

DUODENUM; FLUORESCEINS; HUMANS; HYDROGEN-ION CONCENTRATION; MICROSCOPY, ELECTRON;

EID: 0036233833     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1290/1071-2690(2002)038<0007:HDSFNI>2.0.CO;2     Document Type: Article

References (32)

1. Acid/base transporters in human duodenal enterocytes
2. Regulation of intracellular pH and blood flow in rat duodenal epithelium in vivo
3. Isolation and culturing of highly polarized primary epithelial cells from normal human stomach (antrum) as spheroid-like vesicles
4. Highly polarized primary epithelial cells from human nasopharynx grown as spheroidlike vesicles
5. Superiority of in vitro over in vivo calibrations of BCECF in vascular smooth muscle cells
6. Fluorescence ratio imaging microscopy
7. Parietal cell culture: New models and directions
8. Primary cultures for studies of cell regulation and physiology in intestinal epithelium
9. Proliferation and differentiation of fetal rat intestinal epithelial cells in primary serum free culture
10. pH regulation in isolated in vitro perfused rat colonic crypts
11. 3-secretion
12. 3 cotransport
13. + exchange on the apical side of surface colonocytes using BCECF
14. A histotoxin produced by Salmonella
15. 2 and bicarbonate
16. 3 exchange in rabbit oxyntic cells using fluorescence microscopy
17. - cotransporter and intracellular pH regulation in chicken enterocytes
18. Molecular and functional evidence for electrogenic and electroneutral Na(+)-HCO(3)(-) cotransporters in murine duodenum
19. Identification of transport abnormalities in duodenal mucosa and duodenal enterocytes from patients with cystic fibrosis
20. Membrane potential dependence of Fe(III) uptake by mouse duodenum
21. Massive programmed cell death in intestinal epithelial cells induced by three-dimensional growth conditions: Suppression by mutant c-Hras oncogene expression
22. Establishment of long-term primary cultures of human small and large intestinal epithelial cells
23. Intracellular pH
24. A functional CFTR protein is required for mouse intestinal cAMP-, cGMP- and Ca(2+)-dependent HCO3-secretion
25. i exchange and H delivery in IEC-6 cells
26. Cell and environment interactions in tumor microregions: The multicell spheroid model
27. 3 cotransport in rabbit parietal cells
28. Fluorescent indicators of ion concentrations
29. Intracellular pH in renal tubules in situ: Single cell measurements by confocal laserscan microscopy
30. Intracellular pH measurements in rat duodenal mucosa in vitro using confocal laserscan microscopy
31. Intracellular pH regulation in IEC-6 cells, a cryptlike intestinal cell line
32. Effect of luminal acid on intracellular pH in oxynticopeptic cells in intact frog gastric mucosa