Superiority of in vitro over in vivo calibrations of BCECF in vascular smooth muscle cells

FASEB Journal

Volumn 10, Issue 10, 1996, Pages 1205-1212

  Boyarsky, Gregory ^a,b   Hanssen, Chris ^a   Clyne, Lisa A ^a  

^a UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

^b UTMB at Galveston ^*  (United States)

Author keywords

buffering power; intracellular pH; nigericin; rabbit

Indexed keywords

2,7 BIS(CARBOXYETHYL) 6 CARBOXYFLUORESCEIN; FLUORESCEIN; NIGERICIN;

ACCURACY; ANIMAL CELL; ARTICLE; CALIBRATION; CELL PH; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; VASCULAR SMOOTH MUSCLE;

EID: 0029835972     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fasebj.10.10.8751723     Document Type: Article

References (27)

1. +/nigericin for Calibrating BCECF: I. Estimating steady-state Sntracellular pH. Am. J. Physiol, In press
2. +/nigericin to calibrate BCECF: II. Intrauellular pH-dependence of the correction in vascular smooth muscle cells. Am, J. Physiol. In press
3. Roos, A., and Boron, W. F. (1981) Intracellular pH. Physiol. Rev. 61, 296-434
4. Little, P, J., Cragoe, E. J., Jr., and Bobik, A. (1986) Na-H exchange is a major pathway for Na influx in rat vascular smooth muscle. Am. J. Physiol. 251, C707-C712
5. + exchange in cultured vascular smooth muscles: Evidence for protein kinase C-dependent and -independent pathways. J. Biol. Chem. 262, 5057-5064
6. Halori, N., Fine, B. P., Nakamura, A., Cragoe. E. J., Jr., and Aviv, A. (1987) Angiotensin II effect on cytosolic pH in cultured ral vascular smooth muscle cells. J. Biol. Chem. 262, 5073-5078
7. Korbmacher, C., Helbig, H., Stahl, F., and Wiederholt, M. (1988) Evidence for Na/H exchange and Cl/HC03 exchange in A10 vascular smooth muscle cells. Pfluegers Arch. 412, 29-36
8. + antiporter. J. Biol. Chem. 263, 18023-18029
9. Putnam, R. W.(1990) pH regulator transport systems in a smooth muscle-like cell line. Am. J. Physiol. 258, 470-479
10. i in vascular smooth muscle. Am. J. Physiol. 259, C134-C143
11. + exchanger. J. Membr. Biol. 90, 1-12
12. 3 by stimulating three acid-base transport systems. Nature (London) 337, 648-651
13. 2+. Am. J. Physiol. 259, F269-F278
14. Pouysségur, J., Franchi, A., L'Allemain, G., and Paris, S. (1985) Cytoplasmic pH, a key determinant of growth factor-induced DNA synthesis in quiescent fibroblasts. FEBS Lett. 190, 115-119
15. Boron, W. F. (1986) Inlracellular pH regulation in epithelial cells. Annu. Rev: Physiol. 48, 377-388
16. 3 exchange in rabbit oxyntic cells using fluorescence microscopy. Am. J. Physiol. 253, C30-C36
17. 2+ in lymphocytes J. Cell Biol. 95, 189-196
18. Thomas, J. A., Buchsbaum, R. N., Zimniak, A., and Racker, E. (1979) Intracellular pH measurements in ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ. Biochemistry 18, 2210-2218
19. 3. Am. J. Physiol. 255, C844-C856
20. + exchange by single isolated hepatocytes. Am. J. Physiol. 260, C167-C175
21. Boyarsky, G., Ransom, B., Schlue, W.-R., Davis, M. B. E., and Boron, W. F. (1993) Intracellular pH regulation in single cultured astroeytes from rat forebrain. GLIA 8, 241-248
22. 3) exchange in gastric glands. Am. J. Physiol. 250, G524-C534
23. + antiport. J. Gen. Physiol. 83, 341-369
24. 3 exchange in the ral proximal convoluted tubule. Na-dependenl and -independent modes. J. Gen. Physiol. 89, 581-598
25. 3 exchange in NIH-3T3 fibroblasts. J. Biol. Chem. 269, 4116-4124
26. Wilding, T. J., Cheng, B., and Roos, A. (1992) pH regulation in adult rat carotid body glomus cells. Importance of extracellular pH, sodium, and potassium. J. Gen. Physiol. 100, 593-608
27. Chaillet, J. R., and Boron, W. F. (1985) Intracellular calibration of a pH-sensitive dye in isolated, perfused salamander proximal tubules. J. Gen. Physiol. 86, 765-794