Intracellular pH-measurements in rat duodenal mucosa in vitro using confocal laserscan microscopy

Zeitschrift fur Gastroenterologie

Volumn 35, Issue 4, 1997, Pages 263-270

  Weinlich, M ^a,b   Heydasch, U ^b,c   Starlinger, M ^a   Kinne, R K H ^b  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^c ROSTOCK UNIVERSITY MEDICAL CENTER   (Germany)

Author keywords

BCECF; confocal laserscan microscopy; duodenum; intact mucosa; intracellular pH

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL PH; CONTROLLED STUDY; DUODENUM MUCOSA; LASER MICROSCOPY; MALE; NONHUMAN; PH MEASUREMENT; RAT;

ACID-BASE EQUILIBRIUM; ANIMALS; CALIBRATION; CELL SURVIVAL; CELLS, CULTURED; DUODENUM; INTESTINAL MUCOSA; INTRACELLULAR FLUID; MALE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; RATS; RATS, WISTAR;

EID: 0030990057     PISSN: 00442771     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. 3 cotransport. Am J Physiol 1993; 265: G677-G685
2. 2+/calmodulin-dependent protein kinase II, a brush border membrane protein. Proc Natl Acad Sci 1990; 87: 8990-4
3. Paimela H, Kiviluoto T, Mustonen H, Sipponen P, Kivilaakso E. Tolerance of rat duodenum to luminal acid. Dig Dis Sci 1990; 35: 1244-8
4. Kiviluoto T, Paimela H, Mustonen H, Kivilaakso E. Intracellular pH in isolated Necturus antral mucosa exposed to luminal acid. Gastroenterol 1990; 98: 901-8
5. Kaneko K, Guth PH, Kaunitz JD. In vivo measurement of rat gastric surface cell intracellular pH. Am J Physiol 1991; 261: G548-G552
6. 2 on intracellular pH in in vitro frog gastric mucosa. Am J Physiol 1989; 256: G206-G213
7. Dagher PC, Balsam L, Weber T, Egnor RW, Charney AN. Modulation of chloride secretion in the rat colon by intracellular bicarbonte. Gastroenterol 1992; 103: 102-27
8. Opitz N, Weinlich M, Mooren F et al. Dual wave-length excitation in confocal laser scanning microscopy: Intracelluar pH-measurements with a fluorescent indicator. Biomed Technik 1991; 36: 132-3
9. Pawley J. Handbook of biological confocal microscopy. New York, Plenum Press, 1990
10. + exchange in gastric glands as measured with a cytoplasmic-trapped, fluorescent pH-indicator. Proc Natl Acad Sci 1984; 81: 7436-40
11. Tsien RY. Fluorescent indicators of ion concentrations. Methods Cell Biol 1989; 30: 127-56
12. Weinlich M, Capasso G, Kinne RKH. Intracellular pH in renal tubules in situ: Single-cell measurements by confocal laserscan microscopy. Pflügers Arch 1993; 422: 523-9
13. +/base transport in principal cells characterized by confocal fluorescence imaging. Am J Physiol 1990; 259: C365-C373
14. + exchanger is basolateral. Am J Physiol 1991; 260: C868-C876
15. McNeil PL, Ito S. Gastrointestinal cell plasma membrane wounding and resealing in vivo. Gastroenterol 1989; 96: 1238-48
16. i in oxynticopeptic cells of in vitro frog gastric mucosa. Am J Physiol 1990; 258: G815-G824
17. Yanaka A, Muto H, Ito S, Silen W. Effects of ammonium ion and ammonia on function and morphology of in vitro frog gastric mucosa. Am J Physiol 1993; 265: G277-G288
18. + metabolism and the intracellular pH in isolated perfused rat liver. Biochem J 1993; 293: 667-73
19. 3-transport in the rat proximal convoluted tubules. A sodium-coupled electrogenic process. J Gen Physiol 1985; 86: 613-36
20. Owen CS. Comparison of spectrum-shifting intracellular pH probes 5′(and 6′)-carboxy-10-dimethylamino-3-hydroxyspiro[7H-benco[c]xanthene-7, 1′(3′H)-isobenzofuran]-3′-one and 2′,7′-biscarboxyethyl-5(and 6)-carboxyfluorescein. Analyt Biochem 1992; 204: 65-71
21. Wenzl E, Sjaastad MD, Weintraub WH, Machen TE. Intracellular pH-regulation in IEC-6 cells, a crypt-like intestinal cell line. Am J Physiol 1989; 257: G732-G40
22. 3-) exchange in gastric glands. Am J Physiol 1986; 250: G524-G534
23. Boyarsky G, Hanssen C, Clyne LA. Superiority of in vitro over in vivo calibrations of BCECF in vascular smooth muscle cells. FASEB J 1996; 10: 1205-12