Downregulation of Escherichia coli yfiD expression by FNR occupying a site at -93.5 involves the AR1-containing face of FNR

Molecular Microbiology

Volumn 29, Issue 4, 1998, Pages 1113-1123

  Green, Jeffrey ^a   Baldwin, Mandy L ^a   Richardson, Joanne ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE; TRANSCRIPTION FACTOR;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE REPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; STRUCTURE ACTIVITY RELATION;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; DNA PRIMERS; DNA, BACTERIAL; DOWN-REGULATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; IRON-SULFUR PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); PROTEIN CONFORMATION; TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI; NEGIBACTERIA; UNIDENTIFIED BACTERIOPHAGE;

EID: 0031815910     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.01002.x     Document Type: Article

References (30)

1. Aiba, H., Adhya, S., and Decrombrugghe, B. (1981) Evidence for two functional gal promoters in intact Escherichia coli cells. J Biol Chem 256: 1905-1910.
2. Bell, A., and Busby, S.J.W. (1994) Location and orientation of an activating region in the Escherichia coli transcription factor, FNR. Mol Microbiol 11: 383-390.
3. Court, D. (1993) RNA processing and degradation by RNase III. In Control of mRNA Stability. Belasco, J.G., and Brawerman, G. (eds). New York: Academic Press, pp. 71-116.
4. Gerischer, V., and Durre, P. (1992) Messenger-RNA analysis of the adc gene region of Clostridium acetobutylicum during the shift to sotventogenesis. J Bacteriol 174: 426-433.
5. Green, J., and Baldwin, M.L. (1997a) HlyX, the FNR homologue of Actinobacillus pleuropneumoniae, is a [4Fe-4S]-containing oxygen-responsive transcription regulator that anaerobically activates FNR-dependent Class I promoters via an enhanced AR1 contact. Mol Microbiol 24: 593-605.
6. Green, J., and Baldwin, M.L. (1997b) The molecular basis for the differential regulation of the hlyF-encoded haemolysin of Escherichia coli by FNR and HlyX lies in the improved Activating Region 1 contact of HlyX. Microbiology 143: 3785-3793.
7. Green, J., and Guest, J.R. (1994) Regulation of transcription at the ndh promoter of Escherichia coli by FNR and novel factors. Mol Microbiol 12: 433-444.
8. Green, J., Trageser, M., Six, S., Unden, G., and Guest, J.R. (1991) Characterization of the FNR protein of Escherichia coli, an iron binding transcriptional regulator. Proc R Soc Lond B 244: 137-144.
9. Green, J., Sharrocks, A.D., MacInnes, J.I., and Guest, J.R. (1992) Purification of HlyX, a potential regulator of haemolysin synthesis, and properties of HlyX: FNR hybrids. Proc R Soc Lond B 248: 79-84.
10. Green, J., Bennett, B., Jordan, P., Ralph, E.T., Thomson, A.J., and Guest, J.R. (1996a) Reconstitution of the [4Fe-4S] cluster in FNR and demonstration of the aerobic-anaerobic transcription switch in vitro. Biochem J 316: 887-892.
11. Green, J., Anjum, M.F., and Guest, J.R. (1996b) The ndh-binding protein (Nbp) regulates the ndh gene of Escherichia coli in response to growth phase and identical to Fis. Mol Microbiol 20: 1043-1055.
12. Guest, J.R., Green, J., Irvine, A.S., and Spiro, S. (1996) The FNR modulon and FNR-regulated gene expression. In Regulation and Gene Expression in Escherichia coli. Lin, E.C.C., and Lynch, A.S. (eds). Austin, TX: R.G. Landes, pp. 317-342.
13. Johnson, R.C., Ball, C.A., Pfeffer, D., and Simon, M.I. (1988) Isolation of the gene encoding the Hin recombinatorial enhancer binding protein. Proc Natl Acad Sci USA 85: 3484-3488.
14. Khoroshilova, N., Beinert, H., and Kiley, P.J. (1995) Association of a polynuclear iron-sulfur center with a mutant FNR protein enhances DNA-binding. Proc Natl Acad Sci USA 92: 2499-2505.
15. Lazazzera, B.A., Beinert, H., Khoroshilova, N., Kennedy, M.C., and Kiley, P.J. (1996) DNA-binding and dimerization of the Fe-S containing FNR protein Escherichia coli are regulated by oxygen. J Biol Chem 271: 2762-2768.
16. Lodge, J., Williams, R., Bell, A., Chan, B., and Busby, S. (1990) Comparison of promoter activities in Escherichia coli and Pseudomonas aeruginosa - use of a new broad host range promoter-probe plasmid. FEMS Microbiol Lett 67: 221-225.
17. Lian, C.-J., Rosendal, S., and MacInnes, J.I. (1989) Molecular cloning and characterisation of a haemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae. Infect Immun 57: 3377-3382.
18. MacInnes, J.I., Kim, J.E., Lian, C.-J., and Soltes, G.A. (1990) Actinobacillus pleuropneumoniae hlyX gene homology with the fnr gene of Escherichia coli. J Bacteriol 172: 4587-4592.
19. Marsh, P. (1985) ptac85, an Escherichia coli vector for expression of non-fusion proteins. Nucleic Acids Res 14: 3603.
20. Meng, W., Green, J., and Guest, J.R. (1997) FNR-dependent repression of ndh gene expression requires two upstream FNR-binding sites. Microbiology 143: 1521-1532.
21. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
22. Sambrook, J., Fitsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
23. Savery, N., Rhodius, V., and Busby, S. (1996) Protein-protein interactions during transcription activation: the case of the Escherichia coli cyclic AMP receptor protein. Phil Trans R Soc London B 351: 543-550.
24. Sawers, G. (1993) Specific transcriptional requirements for positive regulation of the anaerobically inducible pfl operon by ArcA and FNR, Mol Microbiol 10: 209-213.
25. Schroder, I, Darie, S., and Gunsalus, R.P. (1993) Activation of the Escherichia coli nitrate reductase (narGHJI) operon by NarL and Fnr requires integration host factor. J Biol Chem 268: 771-774.
26. Scott, S., Busby, S., and Beacham, I. (1995) Transcriptional co-activation at the ansB promoters: involvement of the activating regions of CRP and FNR when bound in tandem. Mol Microbiol 18: 521-531.
27. Silhavy, T.J., Barman, M.L., and Enquist, L.W. (1984) Experiments with Gene Fusions. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
28. Spiro, S., and Guest, J.R. (1987) Regulation and overexpres-sion of the fnr gene of Escherichia coli J Gen Microbiol 133: 3279-3288.
29. Williams, S.M., Savery, N.J., Busby, S.J.W., and Wing, H. (1997) transcription activation at Class I FNR-dependent promoters: identification of the activating surface of FNR and the corresponding contact site in the C-terminal domain of the RNA polymerase a subunit. Nucleic Acids Res 25: 4028-4034.
30. Wing, H. J., Williams, S. M., and Busby, S. J. W. (1995) Spacing requirements for transcription activation by Escherichia coli FNR protein. J Bacteriol 177: 6704-6710.