Microscope-based techniques to study cell adhesion and migration

Nature Cell Biology

Volumn 4, Issue 4, 2002, Pages E91-E96

  Roy, Partha ^a   Rajfur, Zenon ^a   Pomorski, Pawel ^a   Jacobson, Ken ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN;

BLEACHING; CELL ADHESION; CELL FUNCTION; CELL MIGRATION; CELL MOTILITY; CELL STRUCTURE; CHROMATOPHORE; CONTROLLED STUDY; ENERGY TRANSFER; FLUORESCENCE MICROSCOPY; FLUORESCENCE SPECTROSCOPY; LASER MICROSCOPY; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; PHOTOACTIVATION; PRIORITY JOURNAL; REVIEW; TECHNIQUE;

ACTINS; BIOLOGICAL TRANSPORT; CELL ADHESION; CELL MOVEMENT; CYTOLOGICAL TECHNIQUES; ENERGY TRANSFER; HUMANS; LIGHT; MICROSCOPY; MODELS, BIOLOGICAL; SPECTROMETRY, FLUORESCENCE;

EID: 0036228952     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb0402-e91     Document Type: Review

References (80)

1. Assembly and mechanosensory function of focal contacts
2. Focal adhesion - The cytoskeletal connection
3. The composition and dynamics of cell-substratum adhesions in locomoting fish keratocytes
4. Cell-matrix contact structures
5. Bidirectional signalling between the cytoskeleton and integrins
6. Integrin signalling revisited
7. Molecular diversity of cell-matrix adhesions
8. Probing molecular processes in live cells by quantitative multidimensional microscopy
9. GFP-based FRET microscopy in living plant cells
10. Coexisting conformations of fibronectin in cell culture imaged using fluorescence resonance energy transfer
11. Mapping of adherens junction components using microscopic resonance energy transfer imaging
12. Interaction of EGF receptor and Grb2 in living cells visualized by fluorescence resonance energy transfer (FRET) microscopy
13. Fluorescence resonance energy transfer between blue-emitting and red shifted excitation derivatives of the green fluorescent protein
14. Protein-Protein Interaction of FHL3 with FHL2 and Visualization of Their Interaction by Green Fluorescent Proteins (GFP) Two-Fusion Fluorescence Resonance Energy Transfer (FRET)
15. Recent advances in technology for measuring and manipulating cell signals
16. Fluorescence ratio imaging of cyclic AMP in single cells
17. A genetically encoded, fluorescent indicator for cyclic AMP in living cells
18. 2+ based on green fluorescent proteins and calmodulin
19. Localized Rac Activation Dynamics Visualized in Living Cells
20. Spatio-temporal images of growth-factor-induced activation of Ras and Rap1
21. Designing, building, and using a fluorescence recovery after photobleaching instrument
22. Photobleaching GFP reveals protein dynamics inside live cells
23. From fixed to FRAP: Measuring protein mobility and activity in living cells
24. Unravelling Golgi membrane traffic with green fluorescent protein chimeras
25. Dynamics of α-actinin in focal adhesions and stress fibres visualized with α-actinin-green fluorescent protein
26. Caged fluorescent probes
27. Signalling pathways underlying eosinophil cell motility revealed by caging peptides
28. Photoactivation of a signal transduction pathway in living cells
29. Caged protein conjugates and light-directed generation of protein activity: Preparation, photoactivation, and spectroscopic characterization of caged G-actin conjugates
30. Light-directed activation of protein activity from caged protein conjugates
31. Synthesis and applications of heterobifunctional photocleavable cross-linking reagents
32. Caged peptides and proteins by targeted chemical modification
33. Local photorelease of caged thymosin b4 in locomoting keratocytes causes cell turning
34. Chromophore-assisted laser inactivation (CALI): Probing protein function in situ with a high degree of spatial and temporal resolution
35. Essential functions of ezrin in maintenance of cell shape and lamellipodial extension in normal and transformed fibroblasts
36. Chromophore-assisted laser inactivation of proteins is mediated by the photogeneration of free radicals
37. Chromophore-assisted light inactivation and self-organization of microtubules and motors
38. Dissecting the link between stress fibres and focal adhesions by CALI with EGFP-fusion proteins
39. Silicone rubber substrata: A new wrinkle in the study of cell locomotion
40. Flexible substrata for the detection of cellular traction forces
41. Traction forces generated by locomoting keratocytes
42. Imaging the traction stresses exerted by locomoting cells with the elastic substratum method
43. Separation of actomyosin-generated and adhesive traction forces in tethered cells provides a physical basis for gliding and turning locomotion in keratocytes
44. Cell locomotion and focal adhesions are regulated by substrate flexibility
45. Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells
46. Traction force microscopy of migrating normal and H-ras transformed 3T3 fibroblasts
47. Nascent focal adhesions are responsible for the generation of strong propulsive forces in migrating fibroblasts
48. A micromechanical device provides a new bend on fibroblast traction forces
49. Keratocytes pull with similar forces on their dorsal and ventral surfaces
50. Force and focal adhesion assembly: A close relationship studied using elastic micropatterned substrate
51. Direct Observations of the Mechanical Behaviors of the Cytoskeleton in Living Fibroblasts
52. Mechanotransduction across the cell surface and through the cytoskeleton
53. Focal contacts as mechanosensors: Externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism
54. Cell spreading and lamellipodial extension rate is regulated by membrane tension
55. Extracellular matrix rigidity causes strengthening of integrin-cytoskeleton linkages
56. Phosphatidylinositol 4, 5-Bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion
57. Position-dependent linkages of fibronectin- integrin-cytoskeleton
58. Cell movement is guided by the rigidity of the substrate
59. Single molecule nanobioscience
60. Single molecule imaging of green fluorescent proteins in living cells: E-Cadherin forms oligomers on the free cell surface
61. Single-molecule analysis of chemotactic signalling in Dictyoselium cells
62. Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor
63. Quantitation of membrane receptor distributions by image correlation spectroscopy: Concept and application
64. Two-photon image correlation spectroscopy and image cross-correlation spectroscopy
65. Measuring the elastic properties of the biological samples with the AFM
66. Spatially resolved force spectroscopy of biological surfaces using the atomic force microscope
67. Dependence of locally measured cellular deformability on position on the cell, temperature, and cytochalasin B
68. Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by using atomic force microscopy
69. Direct, high-resolution measurement of furrow stiffening during division of adherent cells
70. Dynamic substrates: Modulating the behaviors of attached cells
71. Geometric control of cell life and death
72. Near-field scanning optical microscopy in cell biology
73. Getting a grip: New insights for cell adhesion and traction
74. The green fluorescent protein
75. Fluorescence resonance energy transfer microscopy: A mini review
76. Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes
77. Fluorescence resonance energy transfer detected by scanning near-field optical microscopy
78. Fluorescence lifetime imaging microscopy: Spatial resolution of biochemical processes in the cell